A5088166608- Plant Molecular Biology Research
- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- RNA Research and Splicing
- RNA modifications and cancer
- Plant Stress Responses and Tolerance
- Plant Disease Resistance and Genetics
- Plant Parasitism and Resistance
- Bacteriophages and microbial interactions
- Plant Micronutrient Interactions and Effects
- Agriculture, Plant Science, Crop Management
- Plant Genetic and Mutation Studies
- Phosphorus and nutrient management
- Plant responses to water stress
- Plant tissue culture and regeneration
- RNA and protein synthesis mechanisms
- Insect-Plant Interactions and Control
- Plant-Microbe Interactions and Immunity
- Plant pathogens and resistance mechanisms
- Plant nutrient uptake and metabolism
Adam Mickiewicz University in Poznań
2004-2016
Umeå Plant Science Centre
2016
Swedish University of Agricultural Sciences
2016
Umeå University
2016
University of Copenhagen
2010
Sainsbury Laboratory
2005
John Innes Centre
2005
Arabidopsis microRNA expression regulation was studied in a wide array of abiotic stresses such as drought, heat, salinity, copper excess/deficiency, cadmium excess, and sulfur deficiency. A home-built RT-qPCR mirEX platform for the amplification 289 transcripts used to study their response stresses. Small RNA sequencing, Northern hybridization, TaqMan® assays were performed abundance mature microRNAs. broad on level primary miRNAs (pri-miRNAs) observed. However, stress at microRNAs rather...
Abstract We successfully implemented virus-induced gene silencing (VIGS) in barley (Hordeum vulgare) for the functional characterization of genes required Mla13-mediated resistance toward biotrophic pathogen Blumeria graminis f. sp. hordei. Initially, cultivars were screened their ability to host stripe mosaic virus (BSMV)-VIGS vector by allowing its replication and systemic movement without causing excessive symptoms. Phytoene desaturase leading photobleaching was used as a phenotypic...
Abstract Background Gene silencing vectors based on Barley stripe mosaic virus (BSMV) are used extensively in cereals to study gene function, but nearly all studies have been limited genes expressed leaves of barley and wheat. However since many important aspects plant biology root-expressed we wanted explore the potential BSMV for root tissues. Furthermore, newly completed genome sequence emerging cereal model species Brachypodium distachyon as well increasing amount EST information...
Arabidopsis, miR402 that is encoded within the first intron of a protein-coding gene At1g77230, induced by heat stress. Its upregulation correlates with splicing inhibition and intronic proximal polyA site selection. It suggests not processed from an intron, but rather shorter transcript after selection this intron. Recently, introns active 5΄ splice sites (5΄ss') have been shown to stimulate accumulation miRNAs exons intron-containing MIR genes. In contrast, we observed opposite effect on...
Phosphorus (P) in plants is taken from soil as an inorganic phosphate (Pi) and one of the most important macroelements growth development. Plants actively react to Pi starvation by induced expression transporters, MIR399, MIR827, miR399 molecular sponge - IPS1 genes decreased ubiquitin-conjugating enzyme E2 (PHOSPHATE2 PHO2) sensing transport SPX-MFS genes. The PHO2 protein involved degradation transporters PHT1;1 (from roots) PHO1 roots shoots). leads accumulation shoots. In contrast, pho1...
Tyloses are ingrowths of parenchyma cells into the lumen embolized xylem vessels, thereby protecting remaining from pathogens. They found in heartwood, sapwood, and abscission zones can be induced by various stresses, but their molecular triggers unknown. Here, we report that down-regulation PECTIN METHYLESTERASE1 (PtxtPME1) aspen (Populus tremula × tremuloides) formation tyloses activation oxidative stress. We tested whether any stress-related hormones could induce intact plantlets grown...
Factors affecting splicing of plant U12-dependent introns have been examined by extensive mutational analyses in an vivo tobacco (Nicotiana tabacum) protoplast system using from three different Arabidopsis thaliana genes: CBP20, GSH2, and LD. The results provide evidence that efficiency U12 depends on a combination factors, including UA content, exon bridging interactions between the intron flanking U2-dependent introns, enhancer sequences (ESEs). Unexpectedly, all required adenosine at...
Arabidopsis microRNA162 (miRNA162) level regulation was studied under abiotic stresses, such as drought and salinity. The TaqMan® microRNA assay proved that A. thaliana miRNA162 elevated these confirming its salt responsiveness. promoter region analyses of miRNA162a b genes (MIR162a MIR162b) identified numerous salinity responsive elements. However, our results indicated MIR162a presumably the main locus responsible for mature ath-miRNA162 accumulation stresses tested, MIR162b generally...