A5043186278- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Plant Pathogens and Fungal Diseases
- Dialysis and Renal Disease Management
- Pneumocystis jirovecii pneumonia detection and treatment
- Infectious Diseases and Tuberculosis
- Diagnosis and treatment of tuberculosis
- Yeasts and Rust Fungi Studies
- Infectious Diseases and Mycology
- Central Venous Catheters and Hemodialysis
- Antimicrobial Resistance in Staphylococcus
- Nail Diseases and Treatments
- Acute Kidney Injury Research
- Probiotics and Fermented Foods
- Hemodynamic Monitoring and Therapy
- Pharmacological Effects and Toxicity Studies
- Bacterial Identification and Susceptibility Testing
- Mycotoxins in Agriculture and Food
- Antibiotic Resistance in Bacteria
- Mycorrhizal Fungi and Plant Interactions
- Fungal Biology and Applications
- Pneumonia and Respiratory Infections
- Respiratory and Cough-Related Research
Kuwait University
2016-2025
University of Washington
2009-2024
Population Health Research Institute
2023
McMaster University
2023
Goa Medical College
2023
Mubarak Al Kabeer Hospital
2014-2021
Government Medical College
2018
Kuwait Institute for Medical Specialization
2018
Punjab Institute of Cardiology
2017
Ch. Pervaiz Elahi Institute of Cardiology, Multan
2017
The rapid detection and identification of Candida species in clinical laboratories are extremely important for the management patients with hematogenous candidiasis. presently available culture biochemical methods time-consuming lack required sensitivity specificity. In this study, we have established a seminested PCR (snPCR) using universal species-specific primers serum specimens. outer amplified 3' end 5.8S ribosomal DNA (rDNA) 5' 28S rDNA, including internally transcribed spacer 2...
Candida auris is an emerging yeast pathogen of global significance. Its multidrug-resistant nature and inadequacies conventional identification systems pose diagnostic therapeutic challenges. This study investigated occurrence C. in clinical specimens Kuwait its susceptibility to antifungal agents. Clinical strains isolated during 3.5-year period forming pink-colored colonies on CHROMagar were studied by wet mount examination for microscopic morphology Vitek 2 system. A simple...
Candida auris is an emerging, multidrug-resistant yeast, causing outbreaks in healthcare facilities. Echinocandins are the antifungal drugs of choice to treat candidiasis, as they cause few side effects and resistance rarely found. Previously, immunocompromised patients from Kuwait with C. colonisation or infection were treated echinocandins, within days months, was reported urine isolates. To determine whether development echinocandin due independent introductions resistant strains resulted...
The current study revealed that exposure to smog posed a major source of environmental stress and reduced photosynthesis vigor in plants. Chlorophyll breakdown is aspect sensitivity triggered by altered oxidative stress, an integral photosynthesis. Therefore, the general objective this evaluate molecular ecological impacts chlorophyll degradation Arabidopsis thaliana plants under conditions as evidenced markers, hormonal regulation, chlorophyllase activity. were cultivated growth chamber...
Several clinical observations suggest the superiority of icodextrin compared with 4.25% dextrose in optimizing peritoneal ultrafiltration (UF), but no rigorous controlled evaluation has hitherto been performed. For comparing and during long dwell automated dialysis, a multicenter, randomized, double-blind trial was conducted 92 patients (control, 45; icodextrin, 47) 4-h dialysate to plasma ratio creatinine >0.70 D/D(0) glucose <0.34. Long-dwell net UF efficiency (net volume per gram...
The first outbreak of Candida haemulonii fungemia is described. seven isolates from the blood four neonates were identified by DNA sequencing ribosomal DNA. They all resistant to amphotericin B, fluconazole, and itraconazole. This report highlights emergence C. as an opportunistic pathogen in immunocompromised patients.
A bicarbonate dialysate acidified with citrate (CD) has been reported to have local anticoagulant effect. This study examines the effect of CD on dialysis efficiency, measured as eKt/Vurea, and predialysis concentrations BUN, creatinine, phosphate, beta-2 microglobulin in chronic units.Three outpatient hemodialysis units 142 patients were switched for 6 mo. Using each patient's prior mo regular by acetate (AD) control, eKt/Vurea was compared that CD. Follow-up data 7 after collected from...
To prospectively evaluate the performance of two matrix-assisted laser desorption/ionization time-of-flight mass spectrometry systems (MALDI-TOF MS) for identification clinically significant yeast isolates compared to VITEK 2 system.One hundred and eighty-eight consecutive were analyzed by Bruker Biotyper MS. The results with conventional system. Discrepant resolved direct sequencing rDNA.Accurate 2, MS, MS was 94.1% (177/188), 93.0% (175/188), 92.6% (174/188), respectively. Three not...
Candida glabrata is intrinsically less susceptible to azoles, and resistance echinocandins reduced susceptibility (RS) amphotericin B (AMB) have also been detected. The molecular mechanisms of RS AMB were investigated in C. strains Kuwait by sequence analyses genes involved ergosterol biosynthesis. A total 1,646 isolates tested Etest, results for 12 selected confirmed reference broth microdilution. PCR sequencing three (ERG2, ERG6, ERG11) was performed all with (RS-AMB isolates) 5 wild-type...
With increasing incidence of multidrug-resistant tuberculosis (MDR-TB), accurate drug susceptibility testing (DST) Mycobacterium to first-line drugs has become crucial for proper patient management. We evaluated concordance DST results 70 M. isolates across two phenotypic and molecular methods: BACTEC 460TB, MGIT 960 system, GenoType MTBDRplus DNA sequencing gene segments most commonly implicated in conferring resistance anti-TB drugs. Most (84%) were multidrug-resistant. Twenty-four yielded...
Candida auris, a multidrug-resistant species, has the propensity of nosocomial transmission despite normal decontamination procedures. Here, we describe isolation C auris from patients in various hospitals Kuwait during 2014-2018. Susceptibility to antifungal drugs and molecular basis resistance fluconazole, voriconazole micafungin were also studied.Candida (n = 314) obtained 126 eight studied. All isolates identified by PCR amplification and/or PCR-sequencing ribosomal DNA (rDNA)....
Accurate drug susceptibility testing (DST) of Mycobacterium tuberculosis in clinical specimens and culture isolates to first-line drugs is crucial for diagnosis management multidrug-resistant (MDR-TB). Resistance M. rifampicin mainly due mutations hot-spot region rpoB gene (HSR-rpoB). The prevalence disputed (generally missed by rapid phenotypic DST methods) mutations, which include L511P, D516Y, H526N, H526L, H526S, L533P HSR-rpoB I572F cluster II gene, largely unknown. This study...
Changing trends in incidence and antifungal susceptibility patterns of six Candida species causing candidemia Kuwait between 2006–2017 are reported. A total 2075 isolates obtained from 1448 patients were analyzed. Identity was determined by phenotypic methods confirmed PCR amplification/PCR-sequencing rDNA and/or MALDI-TOF MS. Antifungal Etest. C. albicans accounted for 539 (37.22%) cases followed parapsilosis (n = 502, 34.67%), tropicalis 210, 14.5%), glabrata 148, 10.22%), krusei 27,...
The emerging, often multidrug-resistant