Ali K. Abu‐Alfa
A5010751171- Dialysis and Renal Disease Management
- Lanthanide and Transition Metal Complexes
- Advanced MRI Techniques and Applications
- Medical Imaging and Pathology Studies
- Chronic Kidney Disease and Diabetes
- Ion Transport and Channel Regulation
- Central Venous Catheters and Hemodialysis
- Erythropoietin and Anemia Treatment
- Pancreatic function and diabetes
- Parathyroid Disorders and Treatments
- Hemoglobinopathies and Related Disorders
- Potassium and Related Disorders
- Renal Diseases and Glomerulopathies
- Organ Donation and Transplantation
- Diabetes Treatment and Management
- Blood Pressure and Hypertension Studies
- Renal and related cancers
- HIV-related health complications and treatments
- Disaster Response and Management
- Global Maternal and Child Health
- Healthcare Systems and Reforms
- Iron Metabolism and Disorders
- Methemoglobinemia and Tumor Lysis Syndrome
- MRI in cancer diagnosis
- Pharmacological Effects and Toxicity Studies
American University of Beirut
2011-2024
Yale University
2004-2024
Yale New Haven Hospital
2024
American University of Beirut Medical Center
2012-2023
Baker Heart and Diabetes Institute
2015
King Saud University
2015
Edinburgh Royal Infirmary
2015
University of Glasgow
2015
Northwestern University
1988-2013
Université de Limoges
2002
Treatment of secondary hyperparathyroidism with vitamin D and calcium in patients receiving dialysis is often complicated by hypercalcemia hyperphosphatemia, which may contribute to cardiovascular disease adverse clinical outcomes. Calcimimetics target the calcium-sensing receptor lower parathyroid hormone levels without increasing phosphorus levels. We report results two identical randomized, double-blind, placebo-controlled trials evaluating safety effectiveness calcimimetic agent...
The incidence and prevalence of heart failure (HF) chronic kidney disease (CKD) are increasing, as such a better understanding the interface between both conditions is imperative for developing optimal strategies their detection, prevention, diagnosis, management. To this end, Kidney Disease: Improving Global Outcomes (KDIGO) convened an international, multidisciplinary Controversies Conference titled Heart Failure in CKD. Breakout group discussions included (i) HF with preserved ejection...
Globally, the number of patients undergoing maintenance dialysis is increasing, yet throughout world there significant variability in practice initiating dialysis. Factors such as availability resources, reasons for starting dialysis, timing initiation, patient education and preparedness, modality access, well varied "country-specific" factors significantly affect experiences outcomes. As burden end-stage kidney disease (ESKD) has increased globally, also been a growing recognition...
HIV-positive individuals are at increased risk for kidney disease, including HIV-associated nephropathy, noncollapsing focal segmental glomerulosclerosis, immune-complex and comorbid as well injury resulting from prolonged exposure to antiretroviral therapy or opportunistic infections. Clinical guidelines disease prevention treatment in largely extrapolated studies the general population, do not fully incorporate existing knowledge of unique HIV-related pathways genetic factors that...
In chronic kidney disease, anemia and disordered iron homeostasis are prevalent associated with significant adverse consequences. 2012, Kidney Disease: Improving Global Outcomes (KDIGO) issued an guideline for managing the diagnosis, evaluation, treatment of in disease. Since then, new data have accrued from basic research, epidemiological studies, randomized trials that warrant a re-examination previous recommendations. Therefore, 2019, KDIGO decided to convene 2 Controversies Conferences...
Peritoneal dialysis (PD) catheter-related infections are important risk factors for catheter loss and peritonitis. The 2023 updated recommendations have revised clarified definitions classifications of exit site infection tunnel infection. A new target the overall rate should be no more than 0.40 episodes per year at risk. recommendation about topical antibiotic cream or ointment to has been downgraded. New include suggestion dressing cover treatment duration with emphasis on early clinical...
Na+/H+ exchangers in the brush-border (luminal, apical) membrane of renal proximal tubules are responsible for active, transcellular reabsorption NaHCO3 and NaCl. Although well characterized kinetically, protein that mediates exchange brush border has not been identified. Several exchanger genes, including NHE1, NHE2, NHE3, NHE4, expressed kidney. To identify NHE3 gene product to determine its cellular subcellular localization rabbit kidney, an NHE3-isoform-specific antibody was prepared....
Previous immunochemical studies have shown that NHE3 is an apical Na+/H+ exchanger in some renal epithelia. The purpose of the present study was to develop high-affinity, isoform-specific monoclonal antibodies (MAbs) would be useful for carrying out high-resolution immunocytochemical adult and neonatal mammalian kidney. Three MAbs were developed a fusion protein containing amino acids 702-832 rabbit NHE3. Specificity established by immunoblotting membranes from NHE-deficient LAP cells had...
Despite the high costs of treatment people with kidney disease and associated comorbid conditions, amount reliable information available to guide care such patients is very limited. Some treatments have been assessed in randomized trials, but most trials too small detect effects a magnitude that would be realistic achieve single intervention. Therefore, KDIGO convened an international, multidisciplinary controversies conference titled "Challenges Conduct Clinical Trials Nephrology" identify...
Patients with severely decreased glomerular filtration rate (GFR) (i.e., chronic kidney disease [CKD] G4+) are at increased risk for failure, cardiovascular (CVD) events (including heart failure), and death. However, little is known about the variability of outcomes optimal therapeutic strategies, including initiation replacement therapy (KRT). Kidney Disease: Improving Global Outcomes (KDIGO) organized a Controversies Conference an international expert group in December 2016 to address this...
Several clinical observations suggest the superiority of icodextrin compared with 4.25% dextrose in optimizing peritoneal ultrafiltration (UF), but no rigorous controlled evaluation has hitherto been performed. For comparing and during long dwell automated dialysis, a multicenter, randomized, double-blind trial was conducted 92 patients (control, 45; icodextrin, 47) 4-h dialysate to plasma ratio creatinine >0.70 D/D(0) glucose <0.34. Long-dwell net UF efficiency (net volume per gram...
Cadherins are recognized as the principal mediators of homotypic cellular recognition and play a demonstrated role in morphogenic direction tissue development. We report here identification structurally unique, kidney-specific member cadherin multigene family (Ksp-cadherin). cDNA cloning molecular analysis 130-kDa protein confirmed that it was novel indicated most closely resembled members LI-cadherin/HPT-1 subgroup. The predicted possesses definitive cadherin-specific sequence motifs LDRE,...
The association between kidney disease and cancer is multifaceted complex. Persons with chronic (CKD) have an increased incidence of cancer, both treatments can cause impaired function. Renal issues in the setting malignancy worsen patient outcomes diminish adequacy anticancer treatments. In addition, oncology treatment landscape changing rapidly, data on tolerability novel therapies patients CKD are often lacking. Caring for has become more specialized interdisciplinary, currently requiring...
Mammalian Na + /H exchangers (NHEs) are a family of transport proteins (NHE1–NHE5). To date, the cellular and subcellular localization NHE4 has not been characterized using immunochemical techniques. We purified fusion protein containing portion rat (amino acids 565–675) to use as immunogen. A monoclonal antibody (11H11) was selected by ELISA. It reacted specifically with both 60- 65-kDa polypeptide expressed in NHE4-transfected LAP1 cells. By Western blot analysis, identified 65- 70-kDa...