A5062429518- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
Duke University
2020-2025
Significance K63 ubiquitination of ribosomes serves as a key regulator protein production during cellular exposure to oxidative stress. Defining the structural and functional mechanisms translation regulation would support current understanding critical reprogramming eukaryotic gene expression. Our paper presents an examination structure ubiquitinated ribosomes, revealing that this modification structurally destabilizes proteins involved in binding factors is required trap at...
ABSTRACT Cellular exposure to stress is known activate several translational control pathways through ribosome ubiquitination. However, how unique patterns of ubiquitination act at the site-specific level drive distinct modes translation regulation remains unclear. To further understand complexity these ubiquitin signals, we developed a new targeted proteomics approach quantify modification across ribosome. This method increased sensitivity and throughput current approaches allowed us...