A5091351952- Microbial Fuel Cells and Bioremediation
- Microbial Metabolic Engineering and Bioproduction
- Electrochemical sensors and biosensors
- Anaerobic Digestion and Biogas Production
- Microbial metabolism and enzyme function
- Biofuel production and bioconversion
- Membrane-based Ion Separation Techniques
- Ammonia Synthesis and Nitrogen Reduction
- Molten salt chemistry and electrochemical processes
- Metalloenzymes and iron-sulfur proteins
- Carbon dioxide utilization in catalysis
- Electrochemical Analysis and Applications
- Advanced Photocatalysis Techniques
- Photosynthetic Processes and Mechanisms
University of Tübingen
2021-2023
Karlsruhe Institute of Technology
2015-2020
This paper describes the metabolic engineering of Escherichia coli for anaerobic fermentation glucose to acetoin. Acetoin has well-established applications in industrial food production and was suggested be a platform chemical bio-based economy. However, biotechnological is often hampered by simultaneous formation several end products absence an electron acceptor. Moreover, typical strains are potentially pathogenic. The goal this study overcome these limitations establishing...
The world economies are facing permanently increasing energy demands. At the same time, carbon emissions from fossil sources need to be circumvented minimize harmful effects climate change.
Methanogenesis allows methanogenic archaea to generate cellular energy for their growth while producing methane. Thermophilic hydrogenotrophic species of the genus Methanothermobacter have been recognized as robust biocatalysts a circular carbon economy and are already applied in power-to-gas technology with biomethanation, which is platform store renewable utilize captured dioxide. Here, we generated curated genome-scale metabolic reconstructions three strains investigated differences...
Anode-assisted fermentations offer the benefit of an anoxic fermentation routine that can be applied to produce end-products with oxidation state independent from substrate. The whole cell biocatalyst transfers surplus electrons electrode used as a non-depletable electron acceptor. So far, anode-assisted were shown provide high carbon efficiencies but low space-time yields. This study aimed at increasing yields Escherichia coli-based glucose acetoin. experiments build on obligate respiratory...
Abstract Thermophilic Methanothermobacter spp. are used as model microbes to study the physiology and biochemistry of conversion hydrogen carbon dioxide into methane ( i.e ., hydrogenotrophic methanogenesis), because their short doubling times robust growth with high yields. Yet, a genetic system for these was missing despite intense work four decades. Here, we report establishment tools modification M. thermautotrophicus . We developed modular vector system, which provided shuttle-vector...
Abstract Methanogenesis allows methanogenic archaea (methanogens) to generate cellular energy for their growth while producing methane. Hydrogenotrophic methanogens thrive on carbon dioxide and molecular hydrogen as sole sources. Thermophilic hydrogenotrophic Methanothermobacter spp. have been recognized robust biocatalysts a circular economy are now applied in power-to-gas technology. Here, we generated the first manually curated genome-scale metabolic reconstruction three spp‥ We...
Kohlenstoffdioxid lässt sich mithilfe von Enzymen Mikroorganismen in Chemikalien auch mit hoher Wertschöpfung umwandeln. Für viele Unternehmen aus der Abfall-, Chemie-, Stahl- oder Zementbranche wird diese biotechnologische CO2-Umwandlung künftig einen Weg bieten, relativ kostengünstig ihre Treibhausgas-Emissionen zu senken.
Anodische Elektrofermentation ist eine neue Strategie anaerober Biotechnologie. Sie ermoglicht Umsetzungen, bei denen die mittlere Oxidationsstufe des Endproduktes hoher ist als Substrats. Konventionell werden solche Reaktionen ausschlieslich unter oxischen Prozessbedingungen katalysiert. Dies geht mit einer starken Biomassebildung und einem hohem Energieaufwand einher. Bei der fallt stattdessen als Nebenprodukt lediglich elektrische Energie an, welche universell verwendet kann. Ziel...