A5039401967- Microbial Metabolic Engineering and Bioproduction
- Microbial Fuel Cells and Bioremediation
- Anaerobic Digestion and Biogas Production
- Biofuel production and bioconversion
- Thermochemical Biomass Conversion Processes
- CRISPR and Genetic Engineering
- Insect symbiosis and bacterial influences
- Electrocatalysts for Energy Conversion
- Membrane-based Ion Separation Techniques
- Ammonia Synthesis and Nitrogen Reduction
- CO2 Reduction Techniques and Catalysts
- Carbon dioxide utilization in catalysis
- Granular flow and fluidized beds
- Diet and metabolism studies
- Soil Mechanics and Vehicle Dynamics
- Advanced battery technologies research
- Innovation and Socioeconomic Development
University of Tübingen
2020-2024
University of Stuttgart
2023
BRIEF RESEARCH REPORT article Front. Bioeng. Biotechnol., 27 March 2020 | https://doi.org/10.3389/fbioe.2020.00204
Acetogenic bacteria are rising in popularity as chassis microbes for biotechnology due to their capability of converting inorganic one-carbon (C1) gases organic chemicals. To fully uncover the potential acetogenic bacteria, synthetic biology tools imperative either engineer designed functions or interrogate physiology. Here, we report a genome-editing tool at one-nucleotide resolution, namely base editing, based on CRISPR-targeted deamination. This combines nuclease deactivated Cas9 with...
Our societies must reconsider current industrial practices and find carbon-neutral alternatives to avoid the detrimental environmental effects that come with release of greenhouse gases from fossil-energy carriers.
Genome scale metabolic models (GEMs) and other constraint-based (CBMs) play a pivotal role in understanding biological phenotypes advancing research areas like engineering, human disease modelling, drug discovery, personalized medicine. Despite their growing application, significant challenge remains ensuring the reproducibility of GEMs, primarily due to inconsistent reporting inadequate model documentation results. Addressing this gap, we introduce FROG analysis, community driven initiative...
Methanogenesis allows methanogenic archaea to generate cellular energy for their growth while producing methane. Thermophilic hydrogenotrophic species of the genus Methanothermobacter have been recognized as robust biocatalysts a circular carbon economy and are already applied in power-to-gas technology with biomethanation, which is platform store renewable utilize captured dioxide. Here, we generated curated genome-scale metabolic reconstructions three strains investigated differences...
For Clostridium ljungdahlii, the RNF complex plays a key role for energy conversion from gaseous substrates such as hydrogen and carbon dioxide. In previous study, disruption of RNF-complex genes led to loss autotrophy, while heterotrophy was still possible via glycolysis. Furthermore, it shown that limitation during autotrophy could be lifted by nitrate supplementation, which resulted in an elevated cellular growth ATP yield. Here, we used CRISPR-Cas12a delete: (1) complex-encoding gene...
Abstract Methanogenesis allows methanogenic archaea (methanogens) to generate cellular energy for their growth while producing methane. Hydrogenotrophic methanogens thrive on carbon dioxide and molecular hydrogen as sole sources. Thermophilic hydrogenotrophic Methanothermobacter spp. have been recognized robust biocatalysts a circular economy are now applied in power-to-gas technology. Here, we generated the first manually curated genome-scale metabolic reconstruction three spp‥ We...
Abstract Methanothermobacter has been pivotal in elucidating the biochemistry of hydrogenotrophic methanogenesis. These microbes generate cellular energy to grow by producing methane from hydrogen and carbon dioxide. Recently, this physiological trait was adopted for biotechnology power-to-gas processes store renewable electric form natural gas grid. We established a genetic system genome-scale metabolic model (Microbe Year 2021) support further technology advancement implementation...
Abstract Acetogenic bacteria can convert waste gases into fuels and chemicals. Design of bioprocesses for carbon valorization requires quantification steady-state flows. Here, autotrophic chemostats containing Clostridium autoethanogenum grown on CO 2 H revealed that captured (460 ± 80 mmol/gDCW/day) had a significant distribution to ethanol (54 3 mol% with 2.4 0.3 g/L titer). We were impressed this initial result, but also observed limitations biomass concentration growth rate. Metabolic...
Abstract Acetogenic bacteria are rising in popularity as chassis microbes biotechnology due to their capability of converting inorganic one-carbon (C1) gases organic chemicals. To fully uncover the potential acetogenic bacteria, synthetic-biology tools imperative either engineer designed functions or interrogate physiology. Here, we report a genome-editing tool at one-nucleotide resolution, namely base editing, for based on CRISPR-targeted deamination. This combines nuclease deactivated Cas9...
This paper investigates the parameters impacting product quality in a pilot scale biomass torrefaction reactor. The system analyzed this work was designed and manufactured by Norris Thermal Technologies for use Big Lagoon, California at remote mill site. unit continuous feed reactor with an electrically heated screw, which served dual purpose of heating conveyance. energy mass yields were found to be highly correlated analysis. best predictor both yield study steady state temperature...