James K. Heffernan

ORCID: 0000-0002-6752-9043
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About
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Research Areas
  • Microbial Metabolic Engineering and Bioproduction
  • Anaerobic Digestion and Biogas Production
  • Biofuel production and bioconversion
  • Hybrid Renewable Energy Systems
  • Microbial metabolism and enzyme function
  • Microbial Fuel Cells and Bioremediation
  • Carbon Dioxide Capture Technologies
  • Genomics and Phylogenetic Studies
  • CO2 Reduction Techniques and Catalysts
  • Microbial Community Ecology and Physiology
  • Biomedical and Engineering Education
  • Problem and Project Based Learning
  • Carbon dioxide utilization in catalysis
  • Ionic liquids properties and applications
  • Electrocatalysts for Energy Conversion
  • Ocean Acidification Effects and Responses
  • Algal biology and biofuel production
  • CRISPR and Genetic Engineering
  • Advanced battery technologies research
  • Engineering Education and Curriculum Development
  • Catalysts for Methane Reforming

The University of Queensland
2020-2025

ARC Centre of Excellence in Synthetic Biology
2021-2025

Monash University
2023

Institute of Bioengineering and Nanotechnology
2020

BRIEF RESEARCH REPORT article Front. Bioeng. Biotechnol., 27 March 2020 | https://doi.org/10.3389/fbioe.2020.00204

10.3389/fbioe.2020.00204 article EN cc-by Frontiers in Bioengineering and Biotechnology 2020-03-27

Microbes able to convert gaseous one-carbon (C1) waste feedstocks are increasingly important transition the sustainable production of renewable chemicals and fuels. Acetogens interesting biocatalysts since gas fermentation using Clostridium autoethanogenum has been commercialised. However, most acetogen strains need complex nutrients, display slow growth, not robust for bioreactor fermentations. In this work, we used three different independent adaptive laboratory evolution (ALE) strategies...

10.1016/j.nbt.2024.06.002 article EN cc-by New Biotechnology 2024-06-12

Electrochemical carbon monoxide reduction (CORR) to C2+ products has advantages over electrochemical CO2 conversion (CO2RR) as issues such carbonation, and loss during CO2RR are omitted in CORR due the stability of CO alkaline solutions. Facing common challenges CO2RR, suffers more from mass transport resistance intrinsically lower aqueous solubility. Therefore gas-diffusion electrodes (GDEs) desired boost formation triple phases active sites obtain higher reaction rates. Herein, for first...

10.1016/j.apcatb.2023.122589 article EN cc-by-nc-nd Applied Catalysis B Environment and Energy 2023-03-08

Abstract Gas fermentation by Clostridium autoethanogenum is a commercial process for the sustainable biomanufacturing of fuels and valuable chemicals using abundant, low-cost C1 feedstocks (CO CO2) from sources such as inedible biomass, unsorted nonrecyclable municipal solid waste, industrial emissions. Efforts toward pathway engineering elucidation gene function in this microbe have been limited lack genetic tools to control expression arduous genome methods. To increase pace progress, here...

10.1093/synbio/ysab008 article EN cc-by Synthetic Biology 2021-01-01

Abstract Gas fermentation of CO 2 and H is an attractive means to sustainably produce fuels chemicals. Clostridium autoethanogenum a model organism for industrial ethanol presents opportunity ‐to‐ethanol processes. As we have previously characterized its /H chemostat growth, here use adaptive laboratory evolution (ALE) with the aim improving growth . Seven ALE lineages were generated, all improved specific rates. conducted in presence 2% along generated Evolved lineage D, which showed...

10.1111/1751-7915.14452 article EN cc-by Microbial Biotechnology 2024-04-01

Blue Carbon ecosystems, which include all tidal wetlands, mitigate climate change by capturing and storing carbon dioxide (CO2) from the atmosphere. Most fixation in these systems is thought to be driven plant microbial photosynthesis, whereas chemosynthetic processes are assumed play a minor role. However, ecosystems often contain anoxic environments ideal for microbes such as acetogens. Here, we show that acetogens abundant active mediators of sequestration wetland soils pairing gene-...

10.1101/2025.01.07.631696 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2025-01-08

Climate change and food security are two of our most significant global challenges time. Conventional approaches for production not only produce greenhouse gases but also require extensive land water resources. An alternative is to use gas fermentation convert as feedstocks into microbial protein-rich biomass (single-cell protein). Aerobic methanotrophic (methane-oxidising) hydrogenotrophic (hydrogen-oxidising) bacteria, which using their energy carbon sources, ideal candidates single-cell...

10.1071/ma23007 article EN cc-by-nc-nd Microbiology Australia 2023-03-09

ABSTRACT Microbes able to convert gaseous one-carbon (C1) waste feedstocks are increasingly important transition the sustainable production of renewable chemicals and fuels. Acetogens interesting biocatalysts since gas fermentation using Clostridium autoethanogenum has already been commercialised. However, most acetogen strains need complex nutrients, display slow growth, not robust for routine bioreactor fermentations. In this work, we used three different independent adaptive laboratory...

10.1101/2023.01.28.526018 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2023-01-29

Biogas production from anaerobic digestion is a well-established bioprocess for energy generation, nutrient recovery, and valorisation of waste resources. Typical biogas contains 60% methane 40% carbon dioxide (CH 4 /CO 2 1.5). Removing or altering content facilitates use as biomethane due to its impact on the calorific value. Technologies that effectively remove convert could play central role in transforming This important world transitions into low circular economy. Gas fermentation...

10.2139/ssrn.4098287 article EN SSRN Electronic Journal 2022-01-01

Abstract Acetogenic bacteria can convert waste gases into fuels and chemicals. Design of bioprocesses for carbon valorization requires quantification steady-state flows. Here, autotrophic chemostats containing Clostridium autoethanogenum grown on CO 2 H revealed that captured (460 ± 80 mmol/gDCW/day) had a significant distribution to ethanol (54 3 mol% with 2.4 0.3 g/L titer). We were impressed this initial result, but also observed limitations biomass concentration growth rate. Metabolic...

10.1101/2020.01.23.917666 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2020-01-24

Contribution to the International Chain Elongation Conference 2020 | ICEC 2020. An abstract can be found in right column.

10.18174/icec2020.18019 article EN International Chain Elongation Conference 2020 2020-11-02
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