A5071454359- Virus-based gene therapy research
- Food Allergy and Anaphylaxis Research
- Probiotics and Fermented Foods
- Gut microbiota and health
- Animal Nutrition and Physiology
- Meat and Animal Product Quality
- Protein Hydrolysis and Bioactive Peptides
- RNA Interference and Gene Delivery
- Proteins in Food Systems
- CRISPR and Genetic Engineering
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Pediatric health and respiratory diseases
- Muscle metabolism and nutrition
- Allergic Rhinitis and Sensitization
- RNA modifications and cancer
- Agriculture Sustainability and Environmental Impact
- Plant Disease Resistance and Genetics
- Nuclear Structure and Function
- Barrier Structure and Function Studies
- Glycosylation and Glycoproteins Research
- Diet and metabolism studies
- RNA Research and Splicing
- Phytoestrogen effects and research
- Phytochemical and Pharmacological Studies
Jilin Agricultural University
2015-2025
Beijing Normal University
2024-2025
Second Affiliated Hospital of Xi'an Jiaotong University
2025
Chongqing University
2024
Guangxi University
2019-2020
National Institute for Biological Standards and Control
2006-2019
China National Rice Research Institute
2019
Ministry of Education of the People's Republic of China
2015
Georgia State University
2014
Dalian Polytechnic University
2013
ABSTRACT The type D simian retroviruses cause immunosuppression in macaques and have been reported as a presumptive opportunistic infection patient with AIDS. Previous evidence based on viral interference has strongly suggested that the viruses share common but unknown cell surface receptor three C viruses: feline endogenous virus (RD114), baboon virus, avian reticuloendotheliosis virus. Furthermore, gene for these mapped to human chromosome 19q13.1–13.2. We now report isolation...
This study was developed to provide further information on the intestinal barrier permeability and tight junction protein expression in weaned piglets fed with different levels of soybean agglutinin (SBA). Twenty-five crossbred barrows (Duroc × Landrace Yorkshire) were selected randomly allotted five groups, each group replicates. The control not leguminous products. 0.05, 0.1, 0.15 0.2% SBA added diet form four experimental diets, respectively. After period 7 days (for group), all...
Gene therapy is a rapidly evolving field. So far, there have been >2,400 gene products in clinical trials and four on the market. A prerequisite for producing ensuring their quality safety. This requires appropriately controlled standardized production testing procedures that result consistent safety efficacy. Assuring of lentivirus-based particular presents great challenge because they are cell-based multigene include viral therapeutic proteins as well modified cells. In addition to...
Corn bran has been used as an ingredient for pigs, but the underlying mechanisms that improve gut health is less clear. This study was conducted to investigate effects of dietary corn on growth performance, nutrient digestibility, plasma indices related hormones and immunity, microbiota composition, fermentation products in weaned pigs. A total 60 pigs were allocated 2 treatments, piglets each group received control (CON) diet or 5% (CB) 28 d. Growth immunity evaluated. Microbiota...
Adeno-associated viral (AAV) vectors show great promise for gene therapy because of their excellent safety profile; however, development robust dose-determining assays AAV has presented a significant challenge. With the ultimate goal future harmonization and standardization dose determination assays, we systematically analyzed influence key variables, including sample preparation procedure, choice primers, real-time quantitative PCR (qPCR) target sequences calibration DNA conformation on...
In this 3-year prospective grounded theory study in three pediatric settings, we aimed to develop a conceptualization of best practice health care providers (BPHCPs) interaction with parents children complex, chronic, life-threatening conditions. Analysis semistructured interviews 34 and 80 professionals (HCPs) 88 observation periods HCP/parent interactions indicated that BPHCPs shared broad worldview; values equity, family-centered care, integrity; commitment authentic engagement. engaged...
Soybean allergy represents a health threat to human and animals. Glycinin β-conglycinin, the main storage proteins in soybean, have been identified as major food/feed allergens. The present study was conducted investigate disappearance of immunoreactive glycinin β-conglycinin digestive processes piglets. Twelve crossbred piglets, weaned at 21 days age, were allocated three dietary treatments complete block design, each treatment with four replicates (female/male = 1:1). From day 22–28,...
In this study, we sought to investigate the role of soybean agglutinin (SBA) in mediating membrane permeability and mechanical barrier function intestinal epithelial cells. The IPEC-J2 cells were cultured treated with 0, 0.5, 1.0, 1.5, 2.0, 2.5, or 3.0 mg/mL SBA. Transepithelial electrical resistance (TEER) alkaline phosphatase (AP) activity measured evaluate permeability. results showed a significant decrease TEER values (p < 0.05) time- dose-dependent manner, pronounced increase AP 0.05)....
Soybean allergy presents a health threat to humans and animals. The mechanism by which food/feed allergen β-conglycinin injures the intestinal barrier has not been well understood. In this study, changes of epithelial permeability, integrity, metabolic activity, tight junction (TJ) distribution expression induced were evaluated using IPEC-J2 model. results showed significant decrease trans-epithelial electrical resistance (TEER) (p < 0.001) activity remarkable increase alkaline...
A small number of soybean allergens [including Glycinin (11S) and β-Conglycinin (7S)] in the commercially available corn-soybean meal diet can still cause allergy some weaned piglets, which may be result interaction genetic, nutrition, but specific mechanism is unclear. In this study, 20 allergic piglets non-allergic were selected from 92 by skin sensitization tests, used to examine whole sequence genome. The indicators related humoral cellular immunity, transcriptomics, metabolomics...
Abstract Glycinin and β-conglycinin have been identified as major food/feed allergens. But effects of glycinin on enterocyte migration in piglets are scare. Fifteen weanling (7.06±0.18 kg) General No. 1 barrows, weaned at 28 days, were used. The randomly allotted to three (A, B C) treatments with five replicates. the A group (control group) fed diets without ingredients originating from leguminous products, while or C groups containing purified β-conglycinin, which replaced protein Group by...
The mycotoxin fumonisin is known to be harmful humans and animals, thus it desirable reduce content in crop products. We explored the functions of several genes that function biosynthesis (FUM1, FUM6, FUM8, FUM19, FUM21) Fusarium proliferatum found deletion FUM1, or FUM21 results a severe reduction biosynthesis, while loss FUM19 does not. In addition, fumonisin-deficient strains display significantly decreased pathogenicity. Co-cultivation ΔFUM1, ΔFUM6, ΔFUM8, ΔFUM19 mutants restores...
Gut microbiota ferment non-digestible substances to produce metabolites that accumulate in muscle and influence host metabolism. However, the regulatory mechanisms connecting gut microbiota, metabolites, fat deposition across pig breeds remain unclear. This study explores gut-muscle axis regulating meat quality Chinese Songliao Black Pig (SBP) Large White × Landrace Pigs (LWLDP). Digesta samples from ileum, cecum, rectum of both were analyzed using 16 S rRNA sequencing for microbiome...