A5000668491- Cardiac Arrest and Resuscitation
- Trauma and Emergency Care Studies
- Sepsis Diagnosis and Treatment
- Trauma Management and Diagnosis
- Traumatic Brain Injury and Neurovascular Disturbances
- Emergency and Acute Care Studies
- Simulation-Based Education in Healthcare
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Response and Inflammation
- Mechanical Circulatory Support Devices
- Trace Elements in Health
- Tracheal and airway disorders
- Acute Ischemic Stroke Management
- Neurogenesis and neuroplasticity mechanisms
- Appendicitis Diagnosis and Management
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- MicroRNA in disease regulation
- Thermal Regulation in Medicine
- Adrenal Hormones and Disorders
- Central Venous Catheters and Hemodialysis
- Intensive Care Unit Cognitive Disorders
- Hemodynamic Monitoring and Therapy
- Multiple Sclerosis Research Studies
- Mesenchymal stem cell research
- Vascular anomalies and interventions
CHA University
2013-2024
CHA Bundang Medical Center
2012-2024
Gangnam Severance Hospital
2022
Yonsei University
2010-2022
Government of the Republic of Korea
2014
To evaluate the role of NADPH oxidase-mediated reactive oxygen species (ROS) production in multiple sclerosis pathogenesis, we examined effects apocynin, an oxidase assembly inhibitor, on experimental autoimmune encephalomyelitis (EAE). EAE was induced by immunization with myelin oligodendrocyte glycoprotein (MOG (35-55)) C57BL/6 female mice. Three weeks after initial immunization, mice were analyzed for demyelination, immune cell infiltration, and ROS production. Apocynin (30 mg/kg) given...
Abstract Global cerebral ischemia (GCI) is the leading cause of a poor prognosis even after successful resuscitation from cardiac arrest. Therapeutic induction hypothermia (TH) only proven therapy—and current standard care—for GCI arrest; however, its application has been significantly limited owing to technical difficulties. Mesenchymal stem cells (MSCs) are known suppress neuronal death ischemia. The prevention blood-brain barrier (BBB) disruption not suggested as mechanism MSC treatment...
Abstract Background Sepsis has a high mortality rate, but no specific drug been proven effective, prompting the development of new drugs. Immunologically, sepsis can involve hyperinflammation, immune paralysis, or both, which might pose challenges during development. Recently, mitochondrial transplantation emerged as treatment modality for various diseases involving dysfunction, it never tested sepsis. Methods We isolated mitochondria from L6 muscle cells and umbilical cord mesenchymal stem...
Zinc is an essential element required for cell division, migration, and proliferation. Under zinc-deficient conditions, proliferation differentiation of neural progenitors are significantly impaired. Adipose-derived mesenchymal stem cells (AD-MSCs) multipotent that can differentiate into neurons. The aim this study was to evaluate the effect zinc on AD-MSC differentiation. We initially examined at undifferentiated stage. AD-MSCs showed high rates day 6 in 30 μ M 100 ZnCl 2 . chelation...
The Sepsis-3 criteria introduced the system that uses Sequential Organ-Failure Assessment (SOFA) score to define sepsis. cardiovascular SOFA (CV SOFA) scoring needs modification due change in guideline-recommended vasopressors. In this study, we aimed develop and validate modified CV score.We developed, internally validated, externally validated using suspected infection cohort, sepsis septic shock cohort. primary outcome was 28-day mortality. constructed with consideration of recently...
Objectives The purpose of this study was to investigate the ability sonography identify acute epiglottitis in emergency department. Methods Fifteen patients with a final diagnosis from indirect laryngoscopy by an otolaryngologist were enrolled study. To compare normal epiglottis and epiglottitis, 15 healthy volunteers assigned control group. sonographic appearances pre-epiglottic space recorded. anteroposterior diameter at midpoint both edges transverse view measured all participants....
Intracerebral hemorrhage (ICH) is a critical disease, highly associated with mortality and morbidity. Several studies have demonstrated the beneficial effect of mesenchymal stem cells (MSCs) on ICH, mostly focused their mid-to-long-term effect. Acute hematoma expansion one most important prognostic factors ICH. We hypothesized that MSCs would decrease size in acute based findings few recent researches reporting blood-brain barrier endothelial integrity. Rat ICH models were made using...
Background Renal dysfunction is the most important factor to consider when predicting a patient's risk of developing contrast-induced nephropathy (CIN). Measurement creatinine (Cr) via rapid point-of-care blood urea nitrogen/creatinine testing (POCT-BUN/Cr) determine CIN could potentially reduce time required achieve an accurate diagnosis and initiate complete treatment in emergency department (ED). The aim our study was compare results POCT-BUN/Cr reference laboratory tests for BUN serum...
Immune suppression is known to occur during sepsis. Endotoxin tolerance considered a mechanism of immune in However, the timing and serial changes endotoxin have not been fully investigated. In this study, we investigated polymicrobial sepsis model. Herein, used rat model fecal slurry After induction sepsis, peripheral blood mononuclear cells (PBMCs) splenocytes was measured at various time points (6 h, 12 24 48 72 5 days, 7 days), through measurement TNF-α production after stimulation with...
Several studies have demonstrated the beneficial effect of mesenchymal stem cells (MSCs) on intracerebral hemorrhage (ICH). Enhancement therapeutic efficacy MSCs in ICH is necessary, considering diseases high association with mortality and morbidity. Various preconditioning methods to enhance properties been introduced. We suggested apocynin, a well-known nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor, as novel regimen ICH. Rat models were made using bacterial...
Global cerebral ischemia is a cause of poor prognosis after resuscitation from cardiac arrest. Various attempts have been made to minimize global but none more effective than mild hypothermia induction. A few studies shown the effect mesenchymal stem cells on ischemia, no compared this with or assessed any possible interaction. We aimed show delayed neuronal death and compare hypothermia.Experimental study.Animal research laboratory.Adult male Sprague-Dawley rats weighing 250-300 g.Rats were...
Steroids are currently being used in sepsis, particularly septic shock. However, clinical trials to date have shown contradictory results. This could be attributed the different patient endotypes and steroid doses, which also contributed inconclusive We investigated effects of glucocorticoid therapy on sepsis a polymicrobial model variety settings, such as dose, severity, phase. rat fecal slurry sepsis. First, we optimum dose steroids model. administered doses dexamethasone after induction...
Previously, we have shown that mitochondrial transplantation in the sepsis model has immune modulatory effects. The function could different characteristics dependent on cell types. Here, investigated whether effects of be depending type, from which mitochondria were isolated. We isolated L6 muscle cells, clone 9 liver cells and mesenchymal stem (MSC). tested using vitro vivo models. used LPS stimulation THP-1 cell, a monocyte line, as an model. First, observed changes...
Objectives Recent studies have shown that there may be an interaction between duty cycle and other factors related to the quality of chest compression. Duty represents fraction compression phase. We aimed investigate effect shorter phase on average depth during metronome-guided cardiopulmonary resuscitation. Methods Senior medical students performed 12 sets compressions following guiding sounds, with three down-stroke patterns (normal, fast very fast) four rates (80, 100, 120 140...
Abstract Several studies have demonstrated that excitatory amino acid carrier-1 ( EAAC1 ) gene deletion exacerbates hippocampal and cortical neuronal death after ischemia. However, presently there are no investigating the role of in neurogenesis. In this study, we tested hypothesis reduced cysteine transport into neurons by knockout negatively affects adult neurogenesis under physiological or pathological states. This study used young mice (aged 3–5 months) aged 11–15 either wild-type (WT)...
Current guidelines for cardiopulmonary resuscitation recommend chest compressions (CC) during 50% of the duty cycle (DC) in part because ease with which individuals may learn to achieve it practice. However, no consideration has been given a possible interaction between DC and depth CC, subject recent study. Our aim was determine if is inappropriate sufficient compression female light rescuers.Previously collected CC data, performed by senior medical students guided metronome sounds various...
Objectives To assess how the quality of metronome-guided cardiopulmonary resuscitation (CPR) was affected by chest compression rate familiarised training before performance and to determine a possible mechanism for any effect shown. Design Prospective crossover trial simulated, one-person, chest-compression-only CPR. Setting Participants were recruited from medical school two paramedic schools South Korea. 42 senior students enrolled but five dropped out due physical restraints. Intervention...
Tube thoracostomy (TT) is a commonly performed intensive care procedure. Simulator training may be good alternative method for TT training, compared with conventional methods such as apprenticeship and animal skills laboratory. However, there insufficient evidence supporting use of simulator. The aim this study to determine whether medical simulator associated faster process, without simulator.This simulation study. Eligible participants were emergency medicine residents very few (≤3 times)...