A5005854473- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Petroleum Processing and Analysis
- Enhanced Oil Recovery Techniques
- nanoparticles nucleation surface interactions
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Hydrocarbon exploration and reservoir analysis
- Metal and Thin Film Mechanics
- Malaria Research and Control
- Genomics and Phylogenetic Studies
- High-Temperature Coating Behaviors
- Catalytic Processes in Materials Science
- ZnO doping and properties
- Surface and Thin Film Phenomena
- Computational Drug Discovery Methods
- RNA regulation and disease
- NMR spectroscopy and applications
- Intermetallics and Advanced Alloy Properties
- Asphalt Pavement Performance Evaluation
- Enzyme Structure and Function
- Nanocluster Synthesis and Applications
- Pharmacological Effects of Natural Compounds
Cornell University
2023-2024
Weill Cornell Medicine
2024
Chengdu University of Technology
2024
Howard Hughes Medical Institute
2017-2023
University of Colorado Boulder
2017-2023
Wuhan University of Technology
2022
The Ohio State University
2014-2016
Guiyang College of Traditional Chinese Medicine
2015
Tsinghua University
2005-2010
Devon Energy (Canada)
2003-2005
The biological mode of action artemisinin, a potent antimalarial, has long been controversial. Previously we established yeast model addressing its mechanism and found mitochondria the key in executing artemisinin's action. Here present data showing that artemisinin directly acts on it inhibits malaria similar way as yeast. Specifically, homologues exhibit correlated activities against yeast, with peroxide bridge playing role for their inhibitory both organisms. In addition, showed...
Polycomb repressive complex 2 (PRC2) is a key chromatin modifier responsible for methylation of lysine 27 in histone H3. PRC2 has been shown to interact with thousands RNA species vivo, but understanding the physiological function binding hampered by lack separation-of-function mutants. Here, we use comprehensive mutagenesis and hydrogen deuterium exchange mass spectrometry (HDX-MS) identify critical residues interaction core complexes from Homo sapiens Chaetomium thermophilum, which crystal...
Polycomb-group proteins play critical roles in gene silencing through the deposition of histone H3 lysine 27 trimethylation (H3K27me3) and chromatin compaction. This process is essential for embryonic stem cell (ESC) pluripotency, differentiation, development. Polycomb repressive complex 2 (PRC2) can both read write H3K27me3, enabling progressive spreading H3K27me3 on linear genome. Long-range Polycomb-associated DNA contacts have also been described, but their regulation role remain...
Polycomb-repressive complex 2 (PRC2) is a histone methyltransferase that critical for regulating transcriptional repression in mammals. Its catalytic subunit, EZH2, responsible the trimethylation of H3K27 and also undergoes automethylation. Using mass spectrometry analysis recombinant human PRC2, we identified three methylated lysine residues (K510, K514, K515) on disordered but highly conserved loop EZH2. Methylation these lysines increases PRC2 activity, whereas their mutation decreases...
Abstract Polycomb Repressive Complex 2 (PRC2), an important histone modifier and epigenetic repressor, has been known to interact with RNA for almost two decades. In our previous publication (Long, Hwang et al. 2020), we presented data supporting the functional importance of interaction in maintaining PRC2 occupancy on chromatin, using comprehensive approaches including RNA-binding mutant rChIP-seq assay. Recently, concerns have expressed regarding whether impaired methyltransferase activity...
The dynamic regulation of epigenetic states relies on complex macromolecular interactions. PRC2, the methyltransferase responsible for depositing H3K27me3, interacts with distinct accessory proteins to form mutually exclusive subcomplexes PHF1-PRC2.1, MTF2-PRC2.1, PHF19-PRC2.1, and PRC2.2. functions these are unclear thought be highly redundant. Here we show that PRC2 have roles in repression lineage-specific genes stem cell differentiation. Using a human pluripotent model, engineered...
Abstract Process control and optimization of bitumen froth treatment during oil sands processing require rapid analysis asphaltene content in bitumen, solvent‐tobitumen ratio (S/B), the density solvent‐diluted bitumen. NIR spectroscopy was employed to meet this requirement. The system comprised a spectrometer with no moving parts coupled double‐pass transflectance probe via fiber‐optic cable. Quantitative calibration models were established using partial leastsquares regression latent...
The superfamily of 3'-5' polymerases synthesize RNA in the opposite direction to all other DNA/RNA polymerases, and its members include eukaryotic tRNA(His) guanylyltransferase (Thg1), as well Thg1-like proteins (TLPs) unknown function that are broadly distributed, with family three domains life. Dictyostelium discoideum encodes one Thg1 TLPs (DdiTLP2, DdiTLP3 DdiTLP4). Here, we demonstrate depletion each genes results a significant growth defect, protein catalyzes unique biological...
This paper investigates the properties of dislocations in Pd/MgO(001) interfaces. By constructing ideal and virtual interface structures applying Chen–Mobius inversion method, we obtain interatomic potentials ΦPd–Mg ΦPd–O directly from ab initio adhesive energies. Then, by above potentials, as well using atomistic relaxation molecular dynamics methods, stable are obtained. For simplicity, use a two-dimensional model to provide some clear physical pictures dislocations. There two kinds...
Mitochondrial tRNA (mt-tRNA) 5'-editing was first described more than 20 years ago; however, the candidates for enzymes were only recently identified in a eukaryotic microbe (protist), slime mold Dictyostelium discoideum. In this organism, eight of 18 mt-tRNAs are predicted to be edited based on presence genomically encoded mismatched nucleotides their aminoacyl-acceptor stem sequences. Here, we demonstrate that mt-tRNA occurs at all sites D. discoideum as evidenced by changes sequences...
SUMMARY Polycomb-group proteins play critical roles in gene silencing through the deposition of histone H3 lysine 27 trimethylation (H3K27me3) and chromatin compaction 1-5 . This process is essential for embryonic stem cell (ESCs) pluripotency, differentiation, development. Polycomb repressive complex 2 (PRC2) can both read write H3K27me3, enabling progressive spread H3K27me3 on linear genome 6 Long-range Polycomb-associated DNA contacts have also been described, but their regulation role...
The atomistic simulation of a metal/oxide interface is challenge in surface science and technology. It requires systematic way obtaining the interatomic potentials across interface. In this work, we use Chen–Möbius inversion method to study Ni/Al2O3 interface, get concise general formula, which used extract pair from ab initio adhesive energies. A series checks show that inversed are self-consistent also partially transferable. These prefer treat Al terminated but not so good for O summary,...
The first stage in the conventional hot water process for extracting bitumen from mined oil sand produces a froth containing substantial amounts of emulsified and fine solids. Dilution with sufficient quantity paraffinic solvent, such as hexane, causes aggregation rapid settling droplets, solid particles, precipitated asphaltenes. fraction asphaltenes increases solvent-to-bitumen ratio (S/B). This paper reports results thermogravimetric measurements Athabasca using hexane at S/B values...
Interface structure is a basic problem in interface and surface science. It usually indicated by atomic positions for an ideal interface. But this way sometimes unsuitable mismatch interface, because there are too many atoms under consideration, whose coordinates may confuse our mind understanding the structure. In case, 'dislocation representation' introduced. A misfit dislocation network used as effective representation of However, two questions on topic. How to determine structure? And...