A5063530005- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
- Complement system in diseases
- Lichen and fungal ecology
- Forest Ecology and Biodiversity Studies
- interferon and immune responses
- Forest ecology and management
- Vector-borne infectious diseases
- Venomous Animal Envenomation and Studies
Yale University
2021-2024
Vector Oncology (United States)
2020-2024
National Institute of Allergy and Infectious Diseases
2023
National Institutes of Health
2023
Middlebury College
2018
Post-transcriptional mRNA regulation shapes gene expression, yet how cis-elements and translation interface to regulate stability is poorly understood. We find that the strength of initiation, upstream open reading frame (uORF) content, codon optimality, AU-rich elements, microRNA binding sites, (ORF) length function combinatorially stability. Machine-learning analysis identifies ORF as most important conserved feature regulating decay. Upf1 binds translated untranslated ORFs, which are...
Abstract Salivary complement inhibitors occur in many of the blood feeding arthropod species responsible for transmission pathogens. During feeding, these prevent production proinflammatory anaphylatoxins, which may interfere with and limit formation membrane attack complex could damage gut tissues. are, cases, novel proteins be pharmaceutically useful or display unusual mechanisms that exploited pharmaceutically. Albicin is a potent inhibitor alternative pathway from saliva malaria...
The cis -regulatory elements encoded in a mRNA determine its stability and translational output. While there has been considerable effort to understand the factors driving stability, regulatory frameworks governing control remain elusive. We have developed novel massively parallel reporter assay (MPRA) measure translation, Nascent Peptide Translating Ribosome Affinity Purification (NaP-TRAP). NaP-TRAP measures translation frame specific manner through immunocapture of epitope tagged nascent...
Forests in eastern North America are undergoing rapid compositional changes as they experience novel climate, disturbance, and pest conditions. One striking pattern is the replacement of canopy oaks (Quercus spp.) by mesic, fire-sensitive, shade-tolerant species like red maple (Acer rubrum L.). To gain insight into successional patterns driving stand-level oak replacement, we ask two questions: (i) What spatial association mesophyte recruitment compared with overstory individuals, (ii) How...
Inhibition of the alternative pathway (AP) complement by saliva from Anopheles mosquitoes facilitates feeding blocking production anaphylatoxins C3a and C5a, which activate mast cells leading to plasma extravasation, pain, itching. We have previously shown that albicin, a member SG7 protein family An. Albimanus, blocks AP binding inhibiting function C3 convertase, C3bBb. Here we show SG7.AF, albicin homolog freeborni, has similar potency but is more active in presence properdin, acts...
The cis-regulatory elements encoded in an mRNA determine its stability and translational output. While there has been a considerable effort to understand the factors driving stability, regulatory frameworks governing control remain more elusive. We have developed novel massively parallel reporter assay (MPRA) measure translation, named Nascent Peptide Translating Ribosome Affinity Purification (NaP-TRAP). NaP-TRAP measures translation frame-specific manner through immunocapture of epitope...