A5024745905- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Cancer, Hypoxia, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Heart Failure Treatment and Management
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Cardiovascular Function and Risk Factors
- Traumatic Brain Injury and Neurovascular Disturbances
- SARS-CoV-2 detection and testing
- Sepsis Diagnosis and Treatment
- Cardiovascular and exercise physiology
- Long-Term Effects of COVID-19
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- Neurological Disorders and Treatments
- Ubiquitin and proteasome pathways
- Complement system in diseases
- PARP inhibition in cancer therapy
- Photosynthetic Processes and Mechanisms
- vaccines and immunoinformatics approaches
- Sphingolipid Metabolism and Signaling
- Atrial Fibrillation Management and Outcomes
- Pharmacological Receptor Mechanisms and Effects
- Erythropoietin and Anemia Treatment
- Neuropeptides and Animal Physiology
Centro Cardiologico Monzino
2023-2025
Mario Negri Institute for Pharmacological Research
2020-2023
Istituti di Ricovero e Cura a Carattere Scientifico
2021
Abstract Background Septic shock is characterized by breakdown of the endothelial glycocalyx and damage, contributing to fluid extravasation, organ failure death. Albumin has shown benefit in septic patients. Our aims were: (1) identify relations between circulating levels syndecan-1 (SYN-1), sphingosine-1-phosphate (S1P) (endothelial glycocalyx), VE-cadherin cell junctions), severity disease, survival; (2) evaluate effects albumin supplementation on dysfunction patients with shock. Methods...
Summary Solid tumors are often characterized by a hypoxic microenvironment which contributes, through the hypoxia-inducible factor HIF-1, to invasion-metastasis cascade. Endoplasmic reticulum (ER) stress also leads tumor cells thrive and spread inducing transcriptional translational program, Unfolded Protein Response (UPR), aimed at restoring ER homeostasis. We studied ERO1 alpha (henceforth ERO1), protein disulfide oxidase with tumor-relevant characteristic of being positively regulated...
Abstract The new coronavirus SARS-CoV-2 is the causative agent of COVID-19 pandemic, which so far has caused over 6 million deaths in 2 years, despite vaccines and antiviral medications. Drug repurposing, an approach for potential application existing pharmaceutical products to therapeutic indications, could be effective strategy obtain quick answers medical emergencies. Following a virtual screening campaign on most relevant viral proteins, we identified drug raloxifene, known Selective...
C1 esterase inhibitor (C1INH) is known to exert its inhibitory effect by binding several target proteases of the contact and complement systems. One C1INH's targets comprise mannose-binding lectin (MBL), a critical player in post-stroke pathophysiology. We therefore explored effects recombinant human (rh) plasma derived (pd) C1INH C57BL/6J mice subjected transient occlusion middle cerebral artery (tMCAo), receiving 15U/mouse pd or rhC1INH intravenously, at reperfusion. analyzed compounds'...
The rapid spread of the pandemic caused by SARS-CoV-2 virus has created an unusual situation, with searches for compounds to interfere biological processes exploited virus. Doxycycline, its pleiotropic effects, including anti-viral activity, been proposed as a therapeutic candidate COVID-19 and about twenty clinical trials have started since beginning pandemic. To gain information on activity doxycycline against infection clarify some conflicting data published, we designed in vitro binding...
We developed and validated a technology platform for designing testing peptides inhibiting the infectivity of SARS-CoV-2 spike protein-based pseudoviruses. This integrates target evaluation, in silico inhibitor design, peptide synthesis, efficacy screening. generated cyclic library derived from receptor-binding domain (RBD) protein angiotensin-converting enzyme 2 (ACE2) receptor. The cell-free validation process by ELISA competition assays Surface Plasmon Resonance (SPR) studies revealed...
Circulating mannose-binding lectin (MBL) plays an important role in the progression of tissue damage caused by ischemic events, account its high-affinity, multivalent binding to carbohydrate arrays exposed on damaged endothelium (damage-associated molecular patterns, DAMPS). MBL inhibitors have therefore been proposed as a novel protective therapeutic strategy prevent secondary injury. We developed new, convenient, robust surface plasmon resonance (SPR) assay for vitro screening compounds...
Abstract The pandemic caused by the SARS-CoV-2 has created need of compounds able to interfere with biological processes exploited virus. Doxycycline, its pleiotropic effects, including anti-viral activity, been proposed as a therapeutic candidate for COVID-19 and about twenty clinical trials have started since beginning pandemic. To gain information on activity doxycycline against infection clarify some conflicting data published, we designed in vitro binding tests studies pseudotyped virus...
Introduction: Risk stratification in heart failure (HF) is essential for clinical and therapeutic management. The Metabolic Exercise test data combined with Cardiac Kidney Indexes (MECKI) score a validated prognostic model assessing cardiovascular risk HF patients reduced ejection fraction (HFrEF). From the validation of score, prevalence treated direct oral anticoagulants (DOACs), such as edoxaban, non-valvular atrial fibrillation (NVAF) has been increasing recent years. This study aims to...