A5101903029- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Plant tissue culture and regeneration
- Cancer-related molecular mechanisms research
- GABA and Rice Research
- Transgenic Plants and Applications
- Plant Pathogens and Fungal Diseases
- Insect symbiosis and bacterial influences
- Corneal Surgery and Treatments
- Mycorrhizal Fungi and Plant Interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Plant nutrient uptake and metabolism
- Probiotics and Fermented Foods
- Biofuel production and bioconversion
- RNA modifications and cancer
- Mycotoxins in Agriculture and Food
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Circular RNAs in diseases
- Ocular Surface and Contact Lens
- Corneal surgery and disorders
- Wheat and Barley Genetics and Pathology
- RNA regulation and disease
Chinese Academy of Agricultural Sciences
2016-2025
Institute of Crop Sciences
2016-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Second Affiliated Hospital of Nanchang University
2021-2024
Nanchang University
2021-2024
Sanya University
2022-2024
Ministry of Agriculture and Rural Affairs
2023
Qingdao Agricultural University
2022
Society of Interventional Radiology
2013
Sichuan Agricultural University
2012
Cereals high in amylose content (AC) and resistant starch (RS) offer potential health benefits. Previous studies using chemical mutagenesis or RNA interference have demonstrated that branching enzyme (SBE) plays a major role determining the fine structure physical properties of starch. However, it remains challenge to control commercial lines. Here, we use CRISPR/Cas9 technology generate targeted SBEI SBEIIb rice. The frequencies obtained homozygous bi-allelic mutant lines with indels T0...
Foods high in amylose content and resistant starch (RS) offer great potential to improve human health lower the risk of serious noninfectious diseases. Common wheat (Triticum aestivum L.) is a major staple food crop globally. However, RS contents grains modern varieties are low. Here, we report generation high-amylose through targeted mutagenesis TaSBEIIa winter cv Zhengmai 7698 (ZM) spring Bobwhite by CRISPR/Cas9, respectively. We generated series transgene-free mutant lines either with...
Genome editing technologies have revolutionized the field of plant science by enabling targeted modification genomes and are emerging as powerful tools for both gene functional analyses crop improvement. Although homology-directed repair (HDR) is a feasible approach to achieve precise replacement base substitution in some species, dominance non-homologous end joining pathway low efficiency HDR cells limited its application. Base has emerged an alternative tool HDR-mediated replacement,...
Abstract Wheat ( Triticum aestivum L.) is a staple food crop consumed by more than 30% of world population. Nitrogen (N) fertilizer has been applied broadly in agriculture practice to improve wheat yield meet the growing demands for production. However, undue N application and low use efficiency (NUE) modern varieties are aggravating environmental pollution ecological deterioration. Under nitrogen‐limiting conditions, rice Oryza sativa ) abnormal cytokinin response1 repressor1 are1 mutant...
The recently developed CRISPR (clustered regularly interspaced short palindromic repeats)/Cpf1 system expands the range of genome editing and is emerging as an alternative powerful tool for both plant functional genomics crop improvement. Cpf1-CRISPR RNA (crRNA) produces double strand DNA breaks (DSBs) with long 5'-protruding ends, which may facilitate pairing insertion repair templates through homology-directed (HDR) targeted gene replacement introduction desired elements at specific loci...
Beneficial alleles derived from local landraces or related species, even orthologs other plant are often caused by differences of one several single-nucleotide polymorphisms indels in either the promoter region encoding a gene and account for major agriculturally important traits. Clustered regularly interspaced short palindromic repeats-associated endonuclease Cas9 system (CRISPR/Cas9)-mediated precision genome editing enables targeted allele replacement insertion flag foreign genes at...
Abstract Exploiting novel endogenous glyphosate‐tolerant alleles is highly desirable and has promising potential for weed control in rice breeding. Here, through fusions of different effective cytosine adenine deaminases with nCas9‐NG, we engineered an surrogate two‐component composite base editing system, STCBE‐2, improved C‐to‐T A‐to‐G efficiency expanded the window. Furthermore, targeted a OsEPSPS gene artificial evolution STCBE‐2‐mediated near‐saturated mutagenesis. After hygromycin...
ABSTRACT The development of a single and multiplex gene editing system is highly desirable for either functional genomics or pyramiding beneficial alleles in crop improvement. CRISPR/Cas12i3, which belongs to the Class II Type V‐I Cas system, has attracted extensive attention recently due its smaller protein size less restricted canonical “TTN” protospacer adjacent motif (PAM). However, relatively lower efficiency, Cas12i3‐mediated not yet been documented plants. Here, we fused four 5′...
Wheat (Triticum aestivum L., 2n = 6x 42, AABBDD) is one of the most important food crops in world. CRISPR/Cas12i3, which belongs to type V-I Cas system, has attracted extensive attention recently due its smaller protein size and less-restricted canonical 'TTN' protospacer adjacent motif (PAM). However, relatively lower editing efficacy plants hexaploidy complex nature wheat, Cas12i3/Cas12i3-5M-mediated genome wheat not been documented yet. Here, we report engineering a robust...
Engineering of a new type plant base editor for simultaneous adenine transition and transversion within the editing window will greatly expand scope potential in directed evolution crop improvement. Here, we isolated rice endogenous hypoxanthine excision protein, N-methylpurine DNA glycosylase (OsMPG), engineered two A-to-K (K = G or T) editors, rAKBE01 rAKBE02, by fusing OsMPG its mutant mOsMPG to editor, ABE8e. We further coupled either with transactivation factor VP64 generate rAKBE03...
N1-methyladenosine (m1A) modification is a crucial post-transcriptional regulatory mechanism of messenger RNA (mRNA) in living organisms. Few studies have focused on analysis m1A regulators lower-grade gliomas (LGG). We employed the Nonnegative Matrix Factorization (NMF) technique The Cancer Genome Atlas (TCGA) dataset to categorize LGG patients into 2 groups. These groups exhibited substantial disparities terms both overall survival (OS) and levels infiltrating immune cells. collected...