A5028506726- Streptococcal Infections and Treatments
- Escherichia coli research studies
- Pneumonia and Respiratory Infections
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Infective Endocarditis Diagnosis and Management
- Glycosylation and Glycoproteins Research
- Genomics and Phylogenetic Studies
- Lipid Membrane Structure and Behavior
- Insect Resistance and Genetics
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Entomopathogenic Microorganisms in Pest Control
- Oral microbiology and periodontitis research
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
- Insect Pest Control Strategies
- Insect and Pesticide Research
- Antibiotic Resistance in Bacteria
- Diabetes and associated disorders
Nationwide Children's Hospital
2019-2022
Universidad Nacional Autónoma de México
2016-2021
Type three secretion systems (T3SSs) are virulence determinants employed by several pathogenic bacteria as molecular syringes to inject effector proteins into host cells. Diarrhea-producing enteropathogenic Escherichia coli (EPEC) uses a T3SS colonize the intestinal tract. T3S is highly coordinated process that ensures hierarchical delivery of classes substrates: early (inner rod and needle subunits), middle (translocators), late (effectors). Translocation effectors triggered upon host-cell...
The type III secretion system (T3SS) is a supramolecular machine used by many bacterial pathogens to translocate effector proteins directly into the eukaryotic host cell cytoplasm. Enteropathogenic Escherichia coli (EPEC) an important cause of infantile diarrheal disease in underdeveloped countries. EPEC virulence relies on T3SS encoded within chromosomal pathogenicity island known as locus enterocyte effacement (LEE). In this work, we pursued functional characterization LEE-encoded protein...
Streptococcus oralis is an oral commensal and opportunistic pathogen that can enter the bloodstream cause bacteremia infective endocarditis. Here, we investigated mechanisms of S. binding to mucins using clinical isolates, isogenic mutants glycoconjugates. bound both MUC5B MUC7, with a higher level MUC7. Mass spectrometry identified 128 glycans on MUC5B, MUC7 salivary agglutinin (SAG). MUC7/SAG contained relative abundance Lewis type structures, including b/y, sialyl-Lewis a/x α2,3-linked...
Our studies reveal that the oral colonizer and cause of infective endocarditis Streptococcus oralis subsp. dentisani displays a striking monolateral distribution surface fibrils. Furthermore, our data suggest these fibrils impact structure adherent bacterial chains. Mutagenesis indicate are dependent on three serine-rich repeat proteins (SRRPs), here named fibril-associated protein A (FapA), FapB, FapC, each SRRP forms different fibril with distinct distribution. SRRPs family adhesins have...
Bacterial binding to platelets is a key step in the development of infective endocarditis (IE). Sialic acid, common terminal carbohydrate on host glycans, major receptor for streptococci platelets. So far, all defined interactions between and sialic acid are mediated by serine-rich repeat proteins (SRRPs). However, we identified Streptococcus oralis subsp. IE-isolates that bind but lack SRRPs. In addition some SRRP - isolates also cryptic β-1,4-linked galactose through yet unknown mechanism....
High-density lipoproteins (HDLs) are a group of different subpopulations sialylated particles that have an essential role in the reverse cholesterol transport (RCT) pathway. Importantly, changes protein and lipid composition HDLs may lead to formation with reduced atheroprotective properties. Here, we show Streptococcus pneumoniae pneumolysin (PLY) neuraminidase A (NanA) impair HDL function by causing chemical structural modifications HDLs. The proteomic, lipidomic, cellular, biochemical...
The type III secretion system (T3SS) is a complex molecular device used by several pathogenic bacteria to translocate effector proteins directly into eukaryotic host cells. One remarkable feature of the T3SS its ability secrete different categories in hierarchical manner, ensure proper assembly and timely delivery effectors target In enteropathogenic Escherichia coli, substrate specificity switch from translocator regulated gatekeeper composed SepL, SepD, CesL proteins. Here, we report...
Abstract Bacterial binding to platelets is a key step in the development of infective endocarditis (IE). Sialic acid, common terminal carbohydrate on host glycans, major receptor for streptococci platelets. So far, all defined interactions between and sialic acid are mediated by serine rich repeat proteins (SRRPs). However, we identified Streptococcus oralis subsp. IE-isolates that bind but lack SRRPs. In addition some SRRP-negative isolates also cryptic β-1,4-linked galactose through yet...