A5021508179- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Gallbladder and Bile Duct Disorders
- Renal cell carcinoma treatment
- Neuroendocrine Tumor Research Advances
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Gastrointestinal Tumor Research and Treatment
- Gastrointestinal disorders and treatments
- IgG4-Related and Inflammatory Diseases
- Lung Cancer Research Studies
- Colorectal Cancer Screening and Detection
- Peptidase Inhibition and Analysis
- Single-cell and spatial transcriptomics
- Esophageal and GI Pathology
- Hemophilia Treatment and Research
- Extracellular vesicles in disease
- Urinary Tract Infections Management
- Esophageal Cancer Research and Treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Viral Infections and Vectors
- Protein purification and stability
- Chronic Myeloid Leukemia Treatments
- Abdominal Trauma and Injuries
- Cancer Mechanisms and Therapy
- Bacteriophages and microbial interactions
Asahikawa Medical University
2018-2025
Japan Society for the Promotion of Science
2020
Weatherford College
2020
Molecular Oncology (United States)
2019
Japanese Red Cross Asahikawa Hospital
2016
Akebono (Japan)
2016
Pancreatic intraepithelial neoplasia (PanIN) and intraductal papillary mucinous neoplasms (IPMNs) are pancreatic ductal adenocarcinoma (PDAC) precursor lesions. Detecting these precursors monitoring their progression crucial for early PDAC diagnosis. Digital PCR (dPCR) is a highly sensitive nucleic acid quantification technique offers cost‐effective option patient follow‐up. However, the clinical utility of conventional dPCR restricted by multiplexing constraints, particularly due to...
Objectives The role of long noncoding RNAs (lncRNAs) in pancreatic ductal adenocarcinoma (PDAC) remain unclear. Extracellular vesicle (EV)–encapsulated could be effective targets for liquid biopsy. We aimed to identify previously unknown EV-encapsulated lncRNAs PDAC and establish highly accurate methods isolating EVs. Materials Methods vesicles were isolated using existing newly developed methods, namely, PEViA-UC PEViA-IP, from serum samples 20 patients with PDAC, 22 intraductal papillary...
Background Despite advances in the diagnosis of pancreatic ductal adenocarcinoma (PDA), histological evaluation small and poorly defined masses pancreas is uncomfortable unsafe. Methods We herein report a case early stage PDA, which multiple KRAS mutations were detected juice preoperatively. A hypoechoic area adjacent to portal vein was through endoscopic ultrasound body. evaluated using plasma, by digital PCR. Results Pancreatic duct biopsy cytology performed with no evidence malignancy;...
Abstract Background and study aims Needle tract seeding during endoscopic ultrasound fine-needle biopsy (EUS-FNB) remains a concern. We investigated whether such occurred in patient with pancreatic ductal adenocarcinoma (PDA). Patient methods Surgically resected EUS-FNB-derived specimens were genotyped to determine if gastric wall tumor that emerged 3 years after curative resection of an early-stage PDA was clonally related the original tumor. Results The histologically resembled primary...
The pathogenesis of autoimmune pancreatitis is unknown. In the present study we used high-throughput sequencing with next generation to identify candidate genes associated AIP. A total 27 type 1 AIP patients and 30 healthy blood donors were recruited, DNA samples isolated from their mononuclear cells. sequencer an original custom panel 1031 was detect genetic variants in each sample. Polymorphisms CACNA1S (c.4642C>T), rs41554316, rs2231119, rs1042131, rs2838171, P2RX3 (c.195delG),...
The standard treatment of unresectable biliary tract cancer (BTC) has shown an insufficient response rate (RR). Our retrospective setting revealed that a combination therapy consisting intra-arterial chemotherapy plus radiation (IAC + RT) provided high RR and long-term survival benefits in BTC. This prospective study aimed to test the effectiveness safety IAC RT as first-line therapy. regimen included one-shot with cisplatin, 3–6 months reservoir (5-FU q/week), 50.4 Gy external radiation....
Abstract It is challenging to secure a cytopathologic diagnosis using minute amounts of tumor fluids and tissue fragments. Hence, we developed rapid, accurate, low-cost method for detecting cell-derived DNA from limited specimens samples with low cellularity, detect KRAS mutations in pancreatic ductal carcinomas (PDA) digital PCR (dPCR). The core invention based on the suspension pure water, which causes an osmotic burst; crude could be directly subjected emulsion platform. We examined...
Intra-abdominal hemorrhage caused by blunt hepatic injury is a major cause of morbidity and mortality in patients with abdominal trauma. Some these require laparotomy, rapid decision-making life-saving surgery are essential. Damage control (DC) useful for treating children critical situations. We performed this technique to treat an 8-year-old boy grade IV multiple organ damage. This the first report use ABTHERA Open Abdomen Negative Pressure Therapy System (KCI, now part 3 M Company, San...
A woman in her 60s presented with a small pancreatic head tumor. Imaging studies revealed 13-mm well-defined neuroendocrine neoplasm was suspected, and the patient opted for observation at that time. After 8 months, began experiencing sweating while fasting, blood tests during regular follow-up visits showed hypoglycemia. Hypoglycemia induced fasting test. The tumor exhibited clear features of an insulinoma observation. While neoplasms are often managed through observation, caution should be...
Objectives Unresectable ampullary cancer (AC) is a rare disease entity. The risk factors for recurrent biliary obstruction (RBO) following endoscopic stenting (EBS) unresectable AC remain unknown. In this study we aimed to evaluate the cumulative RBO rate and identify palliative EBS in patients with AC. Methods This multicenter retrospective observational enrolled consecutive who had undergone between April 2011 December 2021. of were evaluated via multivariate analysis. Results analysis...
ゲノム解析技術の進歩を背景に,包括的がんゲノムプロファイリング検査(CGP検査)が実臨床に導入された.これら「プレシジョンメディスン」はがん薬物治療の個別化への道を開く一方で,検査に耐えうる十分量の検体採取が困難な例も存在する.膵胆道癌におけるEUS-FNA/Bでは,デバイスを含めたアプローチの選択や穿刺回数の増加,病理部門との連携による標本作製の工夫により,CGP検査の成功率を高めることも可能である.また近年,EUS-FNA/B検体を用いた核酸解析に加えて蛋白発現の評価を行うことで膵胆道癌診断能の向上を図るなど,多角的な分子診断を目指す取り組みが報告されている.病理診断の補完のみならず,病勢把握や再発予測,新規治療法の探索など,今後のゲノム診療がカバーする領域は多岐にわたることが想定され,さらなる発展が期待される.
Sodium orthovanadate (vanadate) stimulated cAMP phosphodiesterase (PDE) and protein tyrosine kinase (PTK) activities inhibited the phosphotyrosine phosphatase (PTPase) activity in particulate of isolated rat fat pads. Okadaic acid never showed any increase PDE up to 1 microM. Amiloride part both stimulations PTK by vanadate. The had an optimal divalent ion requirement 15 mM Mg+2+2 Mn+2 assay medium was not N-ethylmaleimide, suggesting it be a different type from insulin receptor cytosolic...
EUS-guided biliary drainage (EUS-BD) has been performed increasingly worldwide, especially in patients with malignant tumors which ERCP is difficult, surgically altered GI tracts, and older who are at high risk for surgery. EUS-BD requires high-level skills limited options managing adverse events, particularly when stent migration cholangitis occur. Adverse such as persistent bile leakage from the fistula continuous reflux tract, believed to always have a of severe exacerbation that could...
Introduction: Focal autoimmune pancreatitis (AIP) without elevated serum IgG4 levels presents a diagnostic challenge compared to pancreatic tumors, often leading surgical intervention. Case Presentation: We report case of IgG4-negative focal AIP type 1 in 52-year-old male. Despite repeated endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) and biopsy (FNB), preoperative diagnosis was elusive. Initially, 30-mm hypoechoic mass the head detected by ultrasonography, with dynamic...
Surgical management is not a standard treatment option for metastatic recurrence of pancreatic adenocarcinoma. However, the surgical solitary metastasis useful in selected cases.A 42-year-old woman was referred to our hospital on account epigastric pain associated with mass body. The patient had family history branch duct-type intraductal papillary mucinous neoplasm pancreas.The diagnosed ductal adenocarcinoma (PDA) complicated pancreatitis due duct involvement.The underwent distal...
膵癌のゲノム解析では,4つの遺伝子異常(KRAS,CDKN2A,TP53,SMAD4変異)を高率に認める.最近の研究により,ゲノム,遺伝子発現,タンパク,代謝などの様々なレベルでの異常が明らかとなり,これらのプロファイリングによる個々の患者の発癌や進行パターン,治療効果予測に応用されることが期待される.2019年に適切な薬物治療の提供を目的とした遺伝子パネル検査が保険収載され,本格的なゲノム医療の時代を迎えた.このような新しい診断技術を早期膵癌の発見や遺伝素因など高い発癌リスクを有する人々の発病予防を目指した医療へと拡大するには,多様な分子異常の検出方法の確立が求められる.これら膵癌の分子診断には,膵内の多発病変の存在と腫瘍内の不均一性,癌のクローン進化の理解が重要となる.本稿では,膵癌の発生過程でみられる分子異常を概説し,診療への活用が期待される最新の技術革新について紹介する.