Drought stress affects plant growth and development through several mechanisms, including the induction of oxidative stress. To cope with drought, plants have drought tolerance mechanisms at physiological, biochemical, molecular levels. In this study, effects foliar application distilled water methyl jasmonate (MeJA) (5 50 µM) on responses Impatiens walleriana during two regimes (15 5% soil content, SWC) were investigated. The results showed that response depended concentration elicitor...

We describe the responses of Impatiens walleriana to polyethylene glycol (PEG)-induced physiological drought and potential exogenous salicylic acid (SA) as stress-ameliorating agent. shoot culture was established on 16 different media containing 0–3% PEG 0–3 mM SA. After prolonged (60 days), water relation parameters, oxidative stress indicators, growth shoots and/or SA were recorded. reduced growth, fresh weight, number developed leaves (proliferation rate, PR), relative content,...

Efficient protocols, safe from somaclonal variation, were developed for regeneration of Iris sibirica plants via organogenesis and somatic embryogenesis leaf-base explants cultivated on Murashige Skoog media supplemented with thidiazuron (TDZ, 1.0 mg/l) or 2,4-dichlorophenoxyacetic acid (2,4-D, mg/l). The morphogenic response callus formation efficiency differed significantly between 2,4-D (80.9%) TDZ (67%) morphogenesis induction treatments. induced only organogenic calli, while calli...

Soil salinity is one of the most common abiotic stressors that affects plant growth and development. The aim this work was to investigate influence sodium nitroprusside (SNP), a donor nitric oxide (NO), on physiological response centaury (Centaurium erythraea) shoots grown under stress conditions caused by chloride (NaCl) in vitro. Centaury were first nutrient medium containing different SNP concentrations (50, 100 250 μM) during pretreatment phase. After three weeks, transferred media...

Abstract Background Carrot is an important vegetable crop grown worldwide. The major economic problem in carrot cultivation yellow disease caused by Bactericera trigonica , which induces biotic stress and has the greatest impact on productivity. Comprehensive studies mechanism of defense response to B. infestation have yet be conducted. Methods changes photosynthetic pigments, proline, TPC, H 2 O MDA content, DPPH radical scavenging ability, antioxidant enzyme activity SOD, CAT, POX leaves...

Aquatic plants, just like terrestrial are exposed to oxidative stress. However, their responses still under-researched. In this study, we examined the physiological and antioxidative of an aquatic plant, duckweed (Lemna minor L.), four indole-3-acetic acid (IAA)-degrading -producing Pseudomonas bacteria (Pseudomonas oryzihabitans D1-104/3, P. putida A3-104/5, gessardii C31-106/3 yamanorum C44-104/1) and/or a supraphysiological level IAA (10 mg L−1). Growth characteristics, total...

Impatiens walleriana is a valued ornamental plant sensitive to drought stress. We investigated whether the foliar application of 2mM salicylic acid (SA) can protect potted I. plants from The were divided into: watered plants, drought-stressed treated with SA and SA. number flowers flower buds, relative water content (RWC), contents malondialdehyde (MDA) proline (Pro) activities superoxide dismutases, catalases peroxidases recorded at different time points. Three dehydrin sequences identified...

Viruses are responsible for numerous epidemics in different crops all parts of the world. As a consequence their presence great economic losses being incurred. In addition to development sensitive techniques detection, identification and characterization viruses, substantial attention has also been paid biotechnological methods elimination from plants. this review article, following vitro culture virus ornamental plants presented: meristem culture, thermotherapy, chemotherapy, cryotherapy or...

This study was carried out to examine the drought effect on development, physiological, biochemical and molecular parameters in Impatiens walleriana grown ex vitro. Experiment design included three treatments: Control plants-grown under optimal watering (35%-37% of soil moisture content), drought-stressed plants-non-irrigated reach 15% 5% content recovery plants-rehydrated for four days content. Drought reduced fresh weight, total leaf area, as well dry weight I. shoots. up-regulated...

In this study, the effects of foliar applied methyl jasmonate (MeJA) on drought-stressed Impatiens walleriana growth and leaf physiology parameters: stomatal conductance, chlorophyll, flavonoid, anthocyanin, nitrogen balance index (NBI), were evaluated. These parameters could serve as indicators drought tolerance I. walleriana, a popular horticultural plant worldwide that is very sensitive to drought. The experiment included four treatments: control, plants sprayed with distilled water, 5 µM...

Spiny cheek crayfish (Orconectes limosus L.) were found in the Serbian part of River Danube (August 2004, at 1112 km water course). A description biology, origin and distribution this species, as well potential impact to aquatic ecosystems is presented discussed. This first record invasive Danube. In addition, our results could indicate that there risk for expansion O. other regional waters future.

Bulbs are the main vegetative reproductive organs of Fritillaria meleagris L. In nature, as well in vitro, they become dormant and require low temperatures for further growth during next period. present study, using 10 μM gibberellic acid (GA3), or gibberellin biosynthesis (GA) inhibitors-ancymidol (A) paclobutrazol (P)-the dynamic changes soluble sugars, fructose glucose content, fresh weight sprouting capacity were investigated. F. bulbs cultured on medium with GA3 GA inhibitors 1, 2 5...

Abstract A personalized approach to chemoradiation is important in reducing its potential side effects and identifying a group of patients prone toxicity. MicroRNAs have been shown predictive for radiotoxicity. The goal the study was test if levels miRNA peripheral blood mononuclear cells glioblastoma are associated with toxicity identify peak time point MicroRNA-10b/21/34a were measured 43 without toxicity, at baseline, 15th, 30th fraction by Real-Time quantitative Polymerase Chain...

The rapid production and numerous applications of nanomaterials warrant the necessity importance examining nanoparticles in terms to their environmental biological effects implications. In this study, a water-soluble hyper-harmonized hydroxyl-modified fullerene (3HFWC) on cherry tomato seed germination, seedlings growth, physiological response fruiting was evaluated. Changes photosynthetic pigments content, oxidative stress assessment, aquaporin genes expression plants were studied after...

The effects of exogenous gibberellic acid (GA3) and paclobutrazol on induction somatic embryos in wild type hairy root culture Centaurium erythraea Gillib. were investigated. Both compounds incorperated into 1/2 MS medium at 6 concentrations (0.01, 0.03, 0.1, 0.3, 1.0 3.0 iM). Wild explants cultured the presence GA3 all tested under 16-h photoperiod or darkness decreased number that produced. Paclobutrazol (0.3 mM) induced largest (19.7, 16.5) cultures, respectively. Rooting plants derived...

Abstract Common centaury ( Centaurium erythraea Rafn.) is a plant species that can inhabit saline soils. It known as with high spontaneous regeneration potential in vitro. In the present work we evaluated shoots and roots salinity tolerance of non‐transformed three AtCKX transgenic lines to graded NaCl concentrations (0, 50, 100, 150, 200 mM) Overexpression genes plants resulted an altered cytokinins (CKs) profile leading decline bioactive CK levels and, at same time, increased contents...

Aquaporins comprise a large group of transmembrane proteins responsible for water transport, which is crucial plant survival under stress conditions. Despite the vital role aquaporins, nothing known about this protein family in Impatiens walleriana, commercially important horticultural plant, sensitive to drought stress. In present study, attention given molecular characterization aquaporins I. walleriana and their expression during recovery. We identified four aquaporins: IwPIP1;4,...

Abstract Plant hormones regulate numerous developmental and physiological processes. Abiotic stresses considerably affect production distribution of phytohormones as the stress signal triggers. The homeostasis plant is controlled by their de novo synthesis catabolism. aim this work was to analyse contents total individual groups endogenous cytokinins (CKs) well indole-3-acetic acid (IAA) in AtCKX overexpressing centaury plants grown vitro on graded NaCl concentrations (0, 50, 100, 150, 200...

Abstract Hairy root cultures of lettuce (Lactuca sativa L.) were obtained by inoculation cotyledonary leaves in vitro seedlings (cvs. Nansen and Ljubljanska ledenka) with Agrobacterium rhizogenes A4M70GUS. Approximately 96.7% cvs. 91.2% ledenka inoculated explants produced hairy when they incubated on Murashige Skoog (MS) half-strength medium without plant growth regulators. A total 54% all expressed GUS activity. Every represented an independent transformation event. Line 18 showed the...