A5016034309- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Animal Genetics and Reproduction
- Effects of Environmental Stressors on Livestock
- Sperm and Testicular Function
- Bioenergy crop production and management
- Ovarian function and disorders
- Reproductive System and Pregnancy
- Vector-Borne Animal Diseases
- Kruppel-like factors research
- Animal Disease Management and Epidemiology
- Seed Germination and Physiology
- Mollusks and Parasites Studies
- Animal Behavior and Welfare Studies
- Cancer-related gene regulation
- Skin Protection and Aging
- Sunflower and Safflower Cultivation
- Birth, Development, and Health
- Parasitic Diseases Research and Treatment
- Adipose Tissue and Metabolism
- Cell death mechanisms and regulation
- Vascular Tumors and Angiosarcomas
- Heat shock proteins research
- CRISPR and Genetic Engineering
Universidade Vila Velha
2015-2025
Universidade Estadual Paulista (Unesp)
2012-2021
Universidade Estadual de Goiás
2017
University of Florida
2007-2014
Universidade de São Paulo
2012-2013
In this study, we tested the role of colony-stimulating factor 2 (CSF2) as one regulatory molecules that mediate maternal effects on embryonic development during preimplantation period. Our objective was to verify CSF2 blastocyst yield, determine posttransfer survival, and evaluate properties formed after treatment. vitro, increased percentage oocytes became morulae blastocysts. Blastocysts were treated with tended have a greater number inner cell mass cells had higher ratio trophectoderm...
Heat stress is an important cause of poor development and low survival rates in bovine embryos. Experiments were conducted to test the hypothesis that Bos indicus embryos are more resistant heat than taurus In experiment 1, Nelore Jersey from oocyte pick-up-derived oocytes submitted (96 hours post-insemination, 41 °C, 6 hours), developmental ratios assessed at Day 7 (Day 0 = day fertilization), blastocysts frozen for RNA extraction. Experiment 2 evaluated expression COX2, CDX2, HSF1, PLAC8...
Objectives were to evaluate the role of canonical WNT signaling in development preimplantation embryo. Signaling was activated with 2-Amino-4-(3,4-(methylenedioxy)benzylamino)-6-(3-methoxyphenyl)pyrimidine (AMBMP) and inhibited Dickkopf-related protein 1 (DKK1). Treatment bovine embryos AMBMP at day 5 after insemination decreased blastocyst stage 7 reduced numbers trophectoderm inner cell mass cells. At high concentrations, caused disorganization mass. DKK1 blocked actions but did not affect...
Helminth parasites cause morbidity and mortality in both humans animals. Most anthelmintic drugs used the treatment of parasitic nematode infections act on target proteins or regulate electrical activity neurons muscles. In this way, it can lead to paralysis, starvation, immune attack, expulsion worm. However, current anthelmintics have some limitations that include a limited spectrum across species threat drug resistance, which highlights need for new human veterinary medicine.Present study...
Exposure of bovine conceptuses to colony-stimulating factor 2 (CSF2) from days 5 7 development can increase the percentage transferred that develop term. The purpose this experiment was understand mechanism by which CSF2 increases embryonic and fetal survival. Conceptuses were produced in vitro presence or absence 10 ng/ml after insemination, into cows, flushed uterus at day 15 pregnancy. There a tendency (P=0.07) for proportion cows with recovered conceptus be greater those receiving...
Physiology of the adult can be modified by alterations in prenatal development driven maternal environment. Developmental programming, which established before embryo implants uterus, affect females differently than males. The mechanism sex-specific developmental programming is not known. Here we present evidence that regulatory signals change female embryos male embryos. In particular, actions maternally derived cytokine CSF2 from Day 5 to 7 affected characteristics at 15 for decreased...
SUMMARY The microenvironment of a preimplantation embryo can influence changes in development that affect postnatal phenotypes. One the potential mediators this effect many species is colony‐stimulating factor (CSF2), which increase an embryo's ability to establish pregnancy after its transfer into recipients. Exposure embryos CSF2 during early also pattern later sex‐dependent manner. We therefore hypothesized treatment vitro‐produced with culture would alter birth weight and growth...
Citation Loureiro B, Oliveira LJ, Favoreto MG, Hansen PJ. Colony-stimulating factor 2 inhibits induction of apoptosis in the bovine preimplantation embryo. Am J Reprod Immunol 2011; 65: 578–588 Problem Addition colony-stimulating (CSF2) to culture medium increases post-transfer survival embryos. Here we provide evidence that one mechanism by which CSF2 affects embryo is through inhibition apoptosis. Method study In first experiment, genes and pathways whose expression were regulated...
<title>Abstract</title> Striae distensae, commonly known as stretch marks, are dermal scars resulting from excessive skin stretching and structural remodeling. Despite numerous treatment modalities, patient satisfaction varies significantly, effective management remains a challenge. This study aimed to evaluate with novel serum containing amino acids, vitamins, growth factors for the of striae distensae assess impact individual characteristics, lesion aspects, habits on outcomes. A total 156...
The present study determined the transcriptome profile in Nelore and Holstein oocytes subjected to heat shock during IVM mRNA abundance of selected candidate genes heat-shocked cumulus cells (CC). cows were vivo follicle aspiration. Cumulus-oocyte complexes assigned control (38.5°C, 22h) or (41°C for 12h, followed by 38.5°C 10h) treatment IVM. Denuded bovine microarray analysis. Transcriptome analysis demonstrated 127, nine six differentially expressed between breed, temperature...
Heat shock and tumor necrosis factor-α (TNF-α) induce apoptosis through different mechanisms, with heat acting to cause mitochondrial depolarization caspase-9 activation, while TNF-α acts a receptor-mediated process activate caspase-8. In some cells, however, can also activation. the present study, we tested hypothesis that at 41 °C in bovine preimplantation embryos caspase-9-dependent mechanism. Treatment of either (41 °C) or increased proportion blastomeres were TUNEL positive exhibiting...
Contents Fibroblast growth factor (FGF10) acts at the cumulus oocyte complex, increasing expression of cell expansion‐related genes and competency genes. We tested hypothesis that addition FGF10 to maturation medium improves maturation, decreases percentage apoptotic oocytes increases development blastocyst stage while relative abundance developmentally important ( COX2, CDX2 PLAC8 ). In all experiments, were matured for 22 h in TCM‐199 supplemented with 0, 2.5, 10 or 50 ng/ml FGF10....
The number of antral follicles counted (AFC) by ultrasound is associated with fertility in cattle. Cows higher follicle count (HFC) have performance reproductive‐assisted technologies than cows lower (LFC). In this study, we aimed to define the preantral histology and identify differentially expressed genes (DEGs) using a microarray Nelore Angus heifers HFC LFC. ovaries each animal were scanned an device 12 24 hr after estrus. groups formed based on average total (≥3 mm) breed consistently ±...
Contents The time at which follicles acquire LHR in bovine granulosa cells is the subject of some controversy among researchers. main objective present study was to assess mRNA expression and LRBP ( protein binding), a post‐transcriptional suppressor expression, from two largest around expected follicle deviation Nelore heifers. First, interval between ovulation 20 heifers determined (2.3 ± 0.2 days after ovulation). Ovulation hormonally synchronized, then, were slaughtered on 2, 2.5 3...
Avaliou-se o desenvolvimento pós-descongelamento de embriões bovinos produzidos in vitro (PIV) criopreservados pela técnica vitrificação e congelamento lento em diferentes dias após a fertilização (FIV). Foram realizadas 9 réplicas PIV. Nos 7; 7,5 8 FIV, foram selecionados no estágio blastocisto expandido grau qualidade 1 2 (de acordo com manual IETS). Os (n=472) divididos aleatoriamente para serem por (n=257) ou (n=215). Organizou-se os 6 grupos criopreservação dia: 1) Grupo DT7 (embriões...
The number of antral follicles is considered an important fertility trait because animals with a high follicle count (HFC) produce more oocytes and embryos per cycle. Identification these by genetic markers such as single nucleotide polymorphisms (SNPs) can accelerate selection future generations. aim this study was to perform genome wide association (GWAS) on Nelore Angus heifers HFC low (LFC) counts. groups LFC for genotyping were formed based the average total (≥ 3 mm) counted in each...
Avaliou-se o desenvolvimento pós-descongelamento de embriões bovinos produzidos in vitro (PIV) criopreservados pela técnica vitrificação e congelamento lento em diferentes dias após a fertilização (FIV). Foram realizadas 9 réplicas PIV. Nos 7; 7,5 8 FIV, foram selecionados no estágio blastocisto expandido grau qualidade 1 2 (de acordo com manual IETS). Os (n=472) divididos aleatoriamente para serem por (n=257) ou (n=215). Organizou-se os 6 grupos criopreservação dia: 1) Grupo DT7 (embriões...