A5025367573- Molecular Sensors and Ion Detection
- Supramolecular Self-Assembly in Materials
- Chemical Synthesis and Analysis
- Analytical Chemistry and Sensors
- Electrochemical sensors and biosensors
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Protein Tyrosine Phosphatases
- Vitamin K Research Studies
- Lipid Membrane Structure and Behavior
- Photoreceptor and optogenetics research
- Enzyme function and inhibition
- Parathyroid Disorders and Treatments
- Antimicrobial Peptides and Activities
University of Freiburg
2022-2025
Nature chose phosphates to activate amino acids, where reactive intermediates and complex machinery drive the construction of polyamides. Outside biology, pathways mechanisms that allow spontaneous selective peptide elongation in aqueous abiotic systems remain unclear. Herein we work uncover those by following chemistry aminoacyl phosphate esters, synthetic counterparts adenylates. The esters act as solubility tags, making hydrophobic acids their oligomers soluble water enabling different...
Abstract Despite great progress in the construction of non‐equilibrium systems, most approaches do not consider structure fuel as a critical element to control processes. Herein, we show that amino acid side chains (A, F, Nal) abiotic phosphates can direct assembly and reactivity during transient formation. The fuels bind covalently substrates subsequently influence structures process. We focus on ways which phosphate esters guide formation how cross regulate when constructing assemblies....
Inorganic polyphosphate (polyP) is a ubiquitous biopolymer composed of multiple orthophosphates connected by energy-rich phosphoanhydride bonds. In organisms, polyP digested two types enzymes: exopolyphosphatases, which shorten the chain from ends cleaving off monophosphate units, and endopolyphosphatases, cut internally. While several continuous methods are available to monitor exopolyphosphatase activity, endopolyphosphatase activity assays less common typically involve tedious steps....
Abstract Despite great progress in the construction of non‐equilibrium systems, most approaches do not consider structure fuel as a critical element to control processes. Herein, we show that amino acid side chains (A, F, Nal) abiotic phosphates can direct assembly and reactivity during transient formation. The fuels bind covalently substrates subsequently influence structures process. We focus on ways which phosphate esters guide formation how cross regulate when constructing assemblies....
The pathways and mechanisms that allow spontaneous selective peptide elongation in aqueous abiotic systems remain unclear. Herein we work to uncover those by following the chemistry of aminoacyl phosphates, synthetic counterpart adenylates. Thus, oligomers up dodecamers were synthesized one flask on minute time scale, where consecutive additions activated autonomous phase changes. resulting phases arose from high polar reactive nature which can be dissolved water concentrations 300 mM. Upon...
Polyphosphate kinases (PPKs) have become popular biocatalysts for nucleotide 5'-triphosphate (NTP) synthesis and regeneration. Two unrelated families are described: PPK1 PPK2. They structurally use different catalytic mechanisms. enzymes prefer the usage of adenosine (ATP) polyphosphate (polyP) while PPK2 favour reverse reaction. With emerging PPK in biosynthesis, a deeper understanding their thermodynamic reaction course is need, especially comparison to other kinases. Here, we tested four...
We introduce two water-soluble excited state intramolecular proton transfer (ESIPT) based fluorescent turn-on probes responding to inorganic polyphosphates.
We introduce two water-soluble excited state intramolecular proton transfer (ESIPT) based fluorescent turn-on probes responding to inorganic polyphosphates. These ESIPT enable specific detection of short-chain polyphosphates over a range different other condensed phosphates. The are weakly emissive in their off due the blocking by Cu2+ coordination. Removal copper ion through decomplexation analyte accesses on-state. detect biologically occurring phosphates, pyrophosphate, and nucleotides...
Biochemical energy carriers, such as triphosphates (ATP, GTP) drive selective processes, by incorporating chemical information (Adenine vs. Guanine) in their structure. These recognition elements match with complex machineries through a variety of non-covalent interactions, enabling specific targeting functions. In contrast, most approaches non-equilibrium systems do not consider the structure fuel critical element to control processes. Herein, we show that amino acid side chains (A, F, Nap)...