A5003899534- Enzyme Structure and Function
- Advanced Electron Microscopy Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Photosynthetic Processes and Mechanisms
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Neuroscience and Neuropharmacology Research
- Force Microscopy Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA and protein synthesis mechanisms
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Receptor Mechanisms and Signaling
- Medical Imaging Techniques and Applications
- Mitochondrial Function and Pathology
- S100 Proteins and Annexins
- Protein Kinase Regulation and GTPase Signaling
- Bacteriophages and microbial interactions
- Mass Spectrometry Techniques and Applications
Utrecht University
2022-2024
European Molecular Biology Laboratory
2020
Abstract Cryo electron tomography with subsequent subtomogram averaging is a powerful technique to structurally analyze macromolecular complexes in their native context. Although close atomic resolution principle can be obtained, it not clear how individual experimental parameters contribute the attainable resolution. Here, we have used immature HIV-1 lattice as benchmarking sample optimize for averaging. We systematically tested various such order of projections, different angular...
Mucin 1 (MUC1) is a transmembrane mucin expressed at the apical surface of epithelial cells mucosal surfaces. MUC1 has barrier function against bacterial invasion and well known for its aberrant expression glycosylation in adenocarcinomas. The extracellular domain contains variable number tandem repeats (VNTR) 20 amino acids, which are heavily O -linked glycosylated. Monoclonal antibodies VNTR powerful research tools with applications diagnosis treatment MUC1-expressing cancers. Here, we...
The FLAG-tag/anti-FLAG system is a widely used biochemical tool for protein detection and purification. Anti-FLAG M2 the most popular antibody against FLAG-tag, due to its ease of use, versatility, availability in pure form or as bead conjugate. binds N-terminal, C-terminal internal FLAG-tags binding calcium-independent, but molecular basis FLAG-tag specificity recognition remains unresolved. Here we present an atomic resolution (1.17 Å) structure FLAG peptide complex with Fab anti-FLAG M2,...
Abstract Neuronal network formation is facilitated by recognition between synaptic cell adhesion molecules at the surface. Alternative splicing of provides additional specificity in forming neuronal connections. For teneurin family molecules, alternative EGF-repeats and NHL domain controls protein-protein interactions. Here we present cryo-EM structures compact dimeric ectodomain two teneurin-3 isoforms that harbour splice insert EGF-repeats. This dimer stabilised an EGF8-ABD contact...
Abstract Microtubules (MTs) are key components of the eukaryotic cytoskeleton and essential for intracellular organization, organelle trafficking mitosis. MT tasks depend on binding interactions with MT-associated proteins (MAPs). protein 7 (MAP7) has unusual ability both activating kinesin-1-mediated cargo transport along MTs. Additionally, is reported to stabilize MTs its 112 amino-acid long MT-binding domain (MTBD). Here we investigate structural basis interaction MAP7 MTBD lattice. Using...
Establishment of correct synaptic connections is a crucial step during neural circuitry formation. The Teneurin family neuronal transmembrane proteins promotes cell-cell adhesion via homophilic and heterophilic interactions, required for partner matching in the visual hippocampal systems vertebrates. It remains unclear how individual Teneurins form macromolecular cis- trans-synaptic protein complexes. Here, we present 2.7 Å cryo-EM structure dimeric ectodomain human Teneurin4. reveals...
Abstract The microtubule-associated protein 7 (MAP7) is a involved in cargo transport along microtubules (MTs) by interacting with kinesin-1 through the C-terminal kinesin-binding domain. Moreover, reported to stabilize MT, thereby playing key role axonal branch development. An important element for this latter function 112 amino-acid long N-terminal microtubule-binding domain (MTBD) of MAP7. Here we report NMR backbone and side-chain assignments that suggest primarily alpha-helical...
Abstract Mucin 1 (MUC1) is a transmembrane mucin expressed at the apical surface of epithelial cells different mucosal surfaces including breast and intestine. In gastrointestinal tract, MUC1 has barrier function against bacterial invasion, but can also serve as an entry receptor for pathogenic Salmonella bacteria. Moreover, well known its aberrant expression glycosylation in adenocarcinomas The extracellular domain contains variable number tandem repeats (VNTR) 20 amino acids, which are...
Abstract Neuronal network formation is facilitated by recognition between synaptic cell adhesion molecules (CAMs) at the surface. Alternative splicing of CAMs provides additional specificity in forming neuronal connections. For teneurin family CAMs, alternative EGF-repeats and NHL domain controls protein-protein interactions synapse. Here we present a 3.2 Å cryo-EM structure compact dimeric ectodomain teneurin-3 harbouring both splice inserts. This dimer stabilised an EGF8-ABD contact...
Abstract The FLAG-tag/anti-FLAG system is a widely used biochemical tool for protein detection and purification. Anti-FLAG M2 the most popular antibody against FLAG-tag, due to its ease of use, versatility, availability in pure form or as bead conjugate. binds N-terminal, C-terminal internal FLAG-tags binding calcium-independent, but molecular basis FLAG-tag specificity recognition remains unresolved. Here we present an atomic resolution (1.17 Å) structure FLAG peptide complex with Fab...
Cartilage acidic protein-1 (CRTAC1) is a secreted glycoprotein with roles in development, function and repair of the nervous system. It linked to ischemic stroke, osteoarthritis (long) COVID outcomes, has suppressive activity carcinoma bladder cancer. Structural characterization CRTAC1 been complicated by its tendency form disulfide-linked aggregates. Here, we show that stabilized potassium ions. Using x-ray crystallography, determined structure 1.6 Å. This reveals protein consists...