A5011951251- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Toxin Mechanisms and Immunotoxins
- CAR-T cell therapy research
- Botulinum Toxin and Related Neurological Disorders
- Protein purification and stability
- Hereditary Neurological Disorders
- Neurological disorders and treatments
- Biochemical and Structural Characterization
- Diphtheria, Corynebacterium, and Tetanus
- Transgenic Plants and Applications
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Virology and Viral Diseases
- Viral Infections and Outbreaks Research
- Complement system in diseases
- Bacteriophages and microbial interactions
Ministry of Health of the Russian Federation
2021-2024
Gamalei Institute of Epidemiology and Microbiology
2023
Virus-neutralizing antibodies are one of the few treatment options for COVID-19. The evolution SARS-CoV-2 virus has led to emergence variants with reduced sensitivity some antibody-based therapies. development potent a broad spectrum neutralizing activity is urgently needed. Here we isolated panel single-domain that specifically bind receptor-binding domain S glycoprotein. Three selected exhibiting most robust neutralization potency were used generate dimeric molecules. We observed these...
Single-domain antibodies (sdAbs, VHHs, or nanobodies) are a promising tool for the treatment of both infectious and somatic diseases. Their small size greatly simplifies any genetic engineering manipulations. Such have ability to bind hard-to-reach antigenic epitopes through long parts variable chains, third complementarity-determining regions (CDR3s). VHH fusion with canonical immunoglobulin Fc fragment allows Fc-fusion single-domain (VHH-Fc) significantly increase their neutralizing...
INTRODUCTION. Currently, the primary treatment method for botulism is use of botulinum antitoxin, which causes a number side effects, including allergic reactions. The development medicinal products based on monoclonal antibodies (mAbs), in particular, single-domain mAbs fused to human IgG1 Fc fragment, holds promise toxin poisoning. AIM. This study aimed optimise technology laboratory-scale production mAb fragment (B11-Fc) and post-exposure prophylaxis conduct preclinical efficacy this mAb....
Introduction Numerous agents for prophylaxis of SARS-CoV-2-induced diseases are currently registered the clinical use. Formation immunity happens within several weeks following vaccine administration which is their key disadvantage. In contrast, drugs based on monoclonal antibodies, enable rapid passive immunization and therefore can be used emergency pre- post-exposure COVID-19. However elimination antibody-based from circulation limits usage prolonged pre-exposure prophylaxis. Methods...
Ebola fever is an acute, highly contagious viral disease with a mortality rate that can reach 90%. There are currently no licensed therapeutic agents specific to in the world. Monoclonal antibodies (MAbs) viral-neutralizing activity and high specificity virus glycoprotein (EBOV GP) considered as effective potential antiviral drugs. Over past decade, nanobodies (single-domain antibodies, non-canonical camelid antibodies) have found wide use diagnosis treatment of various infectious...
The influenza virus infection claims ~650,000 lives annually. Taking into account the evolving resistance of pathogen to antiviral drugs and waning effectiveness vaccination among certain populations, new approaches treatment are needed. current study is aimed at obtaining single-domain antibodies (Nanobodies) highly conserved stem domain A hemagglutinin by phage display. Two high-affinity neutralizing clones Nanobodies with a particular specificity were selected; they ensured 100%...
Monoclonal antibodies and recombinant antibody fragments are a very promising therapeutic tool to combat infectious diseases. Due their unique paratope structure, nanobodies (VHHs) hold several advantages over conventional monoclonal antibodies, especially in relation viral infections. Influenza A viruses (IAVs) remain major threat public health. The hemagglutinin (HA) protein is the main protective immunodominant antigen of IAVs. In this study, three broadly reactive (D9.2, E12.2, D4.2)...
Objectives. To develop an effective technology for the cultivation of Chinese hamster ovary (CHO) cells stably producing GamP2C5 antibody which is a component I GamCoviMab candidate drug emergency prevention and therapy infection caused by SARS-CoV-2 virus; to select optimal parameters scale this in production. Methods. The study was performed on CHO (clone 78) cell culture, single-domain fused Fc fragment human IgG1 GamP2C5. Different culture media supplements were used. Cells cultured...
Botulinum neurotoxin (BoNT) is one of the most dangerous bacterial toxins and a potential biological weapon component. BoNT mechanism pathological action based on inhibiting release neurotransmitters from nerve endings. To date, anti-BoNT therapy reduced to use horse hyperimmune serum, which causes many side effects, as well FDA-approved drug BabyBig consists human-derived antibodies (IgG) for infant botulinum treatment. Therapeutics botulism treatment safer monoclonal are undergoing...
Adeno-associated virus vectors are among the most promising ones for delivery of transgenes to various organs and tissues. Recombinant adeno-associated (rAAV) is able transduce both dividing non-dividing cells, has low immunogenicity, provide long-term expression transgenes. Modern technologies make it possible obtain rAAV in vivo use, but they not without drawbacks associated with laboriousness, scalability difficulties, high cost, therefore, improvement technological schemes obtaining an...
Monitoring of the proportion immune individuals and effectiveness vaccination in a population involves evaluation several important parameters, including level virus-neutralising antibodies. In order to combat COVID-19 pandemic, it is essential develop approaches detecting SARS-CoV-2 neutralising antibodies by safe, simple rapid methods that do not require live viruses. To test system for enzyme-linked immunosorbent assay (ELISA) detects potential antibodies, necessary obtain highly purified...
Influenza A and SARS-CoV-2 viruses continue to pose a serious threat public health. In our study we obtained recombinant adeno-associated viral vector expressing the gene of tetramerized single-domain antibodies specific various variants SARSCoV-2 virus influenza viruses. It was shown that single intramuscular administration provides protection against infection COVID-19 in prophylactic mode.
Abstract COVID-19 has caused millions of deaths and many times more infections worldwide, emphasizing the unpreparedness global health system in face new key role for vaccines therapeutics, including virus-neutralizing antibodies, prevention containment disease. Continuous evolution SARS-CoV-2 coronavirus been causing its variants to evade action immune system, which highlighted importance detailed knowledge epitopes already selected potent antibodies. A single-chain antibody (“nanobody”)...
ABSTRACT Virus-neutralizing antibodies are one of the few treatment options for COVID-19. The evolution SARS-CoV-2 virus has led to emergence variants with reduced sensitivity some antibody-based therapies. development potent a broad spectrum neutralizing activity is urgently needed. Here we isolated panel single-domain that specifically bind receptor-binding domain S glycoprotein. Three selected exhibiting most robust neutralization potency were used generate dimeric molecules. We observed...
ABSTRACT Single-domain antibodies (sdAbs, VHHs, or nanobodies) are a promising tool for the treatment of both infectious and somatic diseases. Their small size greatly simplifies any genetic engineering manipulations. Such have ability to bind hard-to-reach antigenic epitopes through long parts variable chains, third complementarity-determining regions (CDR3s). VHH fusion with canonical immunoglobulin Fc fragment allows Fc-fusion single-domain (VHH-Fc) significantly increase their...