A5073569552- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Nanoparticle-Based Drug Delivery
- Genomics and Phylogenetic Studies
- Anaerobic Digestion and Biogas Production
- Biodiesel Production and Applications
- Nanoparticles: synthesis and applications
- Insect symbiosis and bacterial influences
- Microbial Community Ecology and Physiology
- Gold and Silver Nanoparticles Synthesis and Applications
- Enzyme-mediated dye degradation
- Lignin and Wood Chemistry
- Protist diversity and phylogeny
- Polymer Surface Interaction Studies
- Microbial Applications in Construction Materials
- Metalloenzymes and iron-sulfur proteins
- Bacteriophages and microbial interactions
- Building materials and conservation
- Diatoms and Algae Research
- Catalysis and Hydrodesulfurization Studies
- Protein purification and stability
- Enzyme Structure and Function
- Probiotics and Fermented Foods
Thammasat University
2023-2025
North Carolina State University
2019-2025
State Street (United States)
2018
Missouri University of Science and Technology
2016-2018
Abstract Oxidative stability is essential for biodiesel quality. This study examines the effects of copper and water as oxidative inducers on derived from palm, jatropha, sunflower oils. Key properties, including fatty acid methyl ester (FAME) content, iodine number, kinematic viscosity, total were tracked over 22 weeks. The FAME content declined progressively, with palm decreasing 100% to 98.56% after 2 weeks 95.76% Similarly, jatropha dropped 98.44% 98.40% 93.87%, 96.55% 96.42% 91.84% same...
Engineered inorganic nanoparticles (NPs) are essential components in the development of nanotechnologies. For applications nanomedicine, particles need to be functionalized ensure a good dispersibility biological fluids. In many cases however, functionalization is not sufficient: become either coated by corona serum proteins or precipitate out solvent. We show that changing coating magnetic iron oxide NPs using poly-L-lysine (PLL) polymer colloidal stability dispersion improved aqueous...
CtfAB from the extremely thermophilic bacterium, Thermosipho melanesiensis, has been used for in vivo acetone production up to 70°C. This enzyme tentatively identified as rate-limiting step, due its relatively low binding affinity acetate. However, existing kinetic and mechanistic studies on this are insufficient evaluate hypothesis. Here, analysis of purified recombinant T. melanesiensis showed that it a ping pong bi mechanism typical CoA transferases with Km values acetate acetoacetyl-CoA...
Overcoming the oxidation stability of biodiesel has been a significant challenge, especially after an extended storage period. To test major factor affecting quality, eight different conditions consisting water at concentration 500 ppm and tert-butylhydroquinone (TBHQ) concentrations 500, 1000, 2000 ppm, in combination, were added to palm biodiesel, with no-water-added treatment as control. Samples kept dark air-limited room temperature for 52 weeks initial carbon residue 0.05 wt%. Every...
The instability of the protein expression in Pichia pastoris strains has been an issue for various peptide productions. Some modifications to traditional fermentation process could potentially solve problem. Here, we consider a four-stage express CAP2 (cell-penetrating antimicrobial 2) candidate P. KM71H, slow methanol utilization strain. During process, productivity is limited (6.15 ± 0.21 mg/L·h) by low overall consumption (approximately 645 g), which mainly result KM71H. To overcome this...
Renewable alternatives for nonelectrifiable fossil-derived chemicals are needed and plant matter, the most abundant biomass on Earth, provide an ideal feedstock. However, heterogeneous polymeric composition of lignocellulose makes conversion difficult. Lignin presents a formidable barrier to fermentation nonpretreated biomass. Extensive chemical enzymatic treatments can liberate fermentable carbohydrates from biomass, but microbial routes offer many advantages, including concomitant...
Ineffective drug release at the target site is among top challenges for cancer treatment. This reflects facts that interaction with physiological condition can denature active ingredients of drugs, and low delivery to disease microenvironment leads poor therapeutic outcomes. We hypothesize depositing a thin layer bioresponsive polymer on surface nanoparticles would not only protect drugs from degradation but also allow site. Here, we report one-step process prepare coated nanorods (NRs)...
Microbial deconstruction of lignocellulose for the production biofuels and chemicals requires hydrolysis heterogeneous hemicelluloses to access microcrystalline cellulose portion. This work extends previous in vivo vitro efforts characterize hemicellulose utilization by integrating genomic reconstruction, transcriptomic data, operon structures, biochemical characteristics key enzymes understand deployment functionality hemicellulases extreme thermophile Caldicellulosiruptor bescii .
The genome sequences of three extremely thermophilic, lignocellulolytic Caldicellulosiruptor species were closed, improving previously reported multiple-contig assemblies. All 14 classified spp. now have closed genomes. Genome closure will enhance bioinformatic analysis the species, including identification carbohydrate-active enzymes (CAZymes) and comparison against other microorganisms.
Caldicellulosiruptor species hold promise as microorganisms that can solubilize the carbohydrate portion of lignocellulose and subsequently convert fermentable sugars into bio-based chemicals fuels. Members genus have surface layer (S-layer) homology domain-associated glycoside hydrolases (SLH-GHs) mediate attachment to biomass well hydrolysis carbohydrates. bescii , most studied member genus, has only one SLH-GH.
Reported here are complete genome sequences for two anaerobic, thermophilic bacteria isolated from wheat straw, i.e., the (hemi)cellulolytic Thermoclostridium stercorarium subspecies strain RKWS1 (3,029,933 bp) and hemicellulolytic Thermoanaerobacter species RKWS2 (2,827,640 bp). Discovery of indigenous thermophiles in plant biomass suggests that high-temperature microorganisms more ubiquitous than previously thought.
This research aimed to examine the effects of an acidic environment on mechanical properties and durability bio-mortar (BM) encapsulated with Bacillus subtilis bacteria, in comparison normal mortar (NM). The results at 28 days indicated that both 3% 6% HCl significantly increased compressive strength BM by 25% 50%, respectively, compared NM. However, when 11% was introduced, decreased 50% lower than Furthermore, water absorption rate 33% mass loss for comparable, whereas HCl, experienced a...