A5051395140- Microbial Community Ecology and Physiology
- Wastewater Treatment and Nitrogen Removal
- Microbial Fuel Cells and Bioremediation
- Legume Nitrogen Fixing Symbiosis
- Protist diversity and phylogeny
- Anaerobic Digestion and Biogas Production
- Ammonia Synthesis and Nitrogen Reduction
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Metalloenzymes and iron-sulfur proteins
Norwegian University of Life Sciences
2017-2023
University of Freiburg
2023
The strong greenhouse gas nitrous oxide (N2O) can be emitted from wastewater treatment systems as a byproduct of ammonium oxidation and the last intermediate in stepwise reduction nitrate to N2 by denitrifying organisms. A potential strategy reduce N2O emissions would enhance activity reductase (NOS) microbial community. survey existing literature on denitrification showed that reducing capacity (VmaxN2O→N2) exceeded produce (VmaxNO3→N2O) factor 2–10. This suggests an effective sink for N2O,...
Summary Ammonia oxidising bacteria (AOB) are thought to emit more nitrous oxide (N 2 O) than ammonia archaea (AOA), due their higher N O yield under oxic conditions and denitrification in response oxygen (O ) limitation. We determined the kinetics of growth turnover nitric (NO) at low cell densities Nitrosomonas europaea Nitrosopumilus maritimus (AOA) during gradual depletion TAN (NH 3 + . Half‐saturation constants for were similar those by others, except half‐saturation constant ammonium N....
When oxygen becomes limiting, denitrifying bacteria must prepare for anaerobic respiration by synthesizing the reductases NAR (NO3- → NO2-), NIR (NO2- NO), NOR (2NO N2O), and NOS (N2O N2), either en bloc or sequentially, to avoid entrapment in anoxia without energy. Minimizing metabolic burden of this precaution is a plausible fitness trait, we show that model denitrifier Paracoccus denitrificans achieves all cells, while only minority synthesize NIR. Phenotypic diversification with regards...
Bradyrhizobia are common members of soil microbiomes and known as N2 -fixing symbionts economically important legumes. Many also denitrifiers, which can act sinks or sources for O. Inoculation with compatible rhizobia is often needed optimal -fixation, but the choice inoculant may have consequences O emission. Here, we determined phylogeny denitrification capacity Bradyrhizobium strains, most them isolated from peanut-nodules. Analyses genomes end-points showed that all were only ~1/3 could...
Abstract Inoculating agricultural soils with nitrous oxide respiring bacteria (NRB) can reduce N2O-emission, but would be impractical as a standalone operation. Here we demonstrate that digestates obtained after biogas production are suitable substrates and vectors for NRB. We show indigenous NRB in grew to high abundance during anaerobic enrichment under N2O. Gas-kinetics meta-omic analyses showed these NRB’s, recovered metagenome-assembled genomes (MAGs), by harvesting fermentation...
Abstract Bioengineering soil metabolism by inoculation is an emerging approach to enhance plant growth and strengthen specific functions such as N 2 O reduction in order reduce climate forcing. The use of organic wastes substrates carriers microbial biomass has proven be a viable improving effectiveness economic viability. A key factor the success this lies selection microbes capable survival both well soils, which are tolerant rapid environmental fluctuations fertilisations involve. dual...
Summary Bradyrhizobia are common members of soil microbiomes and known as N 2 -fixing symbionts economically important legumes. Many also denitrifiers, which can act sinks or sources for O. Inoculation with compatible rhizobia is often needed optimal -fixation, but the choice inoculant may have consequences O emission. Here, we analyzed phylogeny denitrification capacity Bradyrhizobium strains, most them isolated from peanut-nodules. All were dinitrifiers, only ~ 1/3 could reduce while...
Abstract Inoculating agricultural soils with N 2 O-respiring bacteria (NRB) can reduce O-emissions, but would be impractical as a standalone operation. Here we demonstrate that digestates obtained after biogas production are suitable substrates and vectors for NRB. We show indigenous NRB in grew to high abundance during anaerobic enrichment under O. Gas-kinetics meta-omic analyses showed these NRB's, recovered metagenome-assembled genomes (MAGs), by harvesting fermentation intermediates of...