A5019914255- Protein Structure and Dynamics
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Alzheimer's disease research and treatments
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Antimicrobial Peptides and Activities
- Heat shock proteins research
- Computational Drug Discovery Methods
- Supramolecular Self-Assembly in Materials
- Glycosylation and Glycoproteins Research
- Nuclear Structure and Function
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Protein Interaction Studies and Fluorescence Analysis
- Mitochondrial Function and Pathology
- Genetic Neurodegenerative Diseases
- Drug Transport and Resistance Mechanisms
- Biochemical and Molecular Research
Institute of Protein Research
2016-2024
It has been demonstrated using Aβ40 and Aβ42 recombinant synthetic peptides that their fibrils are formed of complete oligomer ring structures. Such structures have a diameter about 8-9 nm, an height 2- 4 internal the 3-4 nm. Oligomers associate in fibril such way they interact with each other, overlapping slightly. There differences packing oligomers Aβ peptides. The principal difference is degree orderliness ring-like leads to generation morphologically different fibrils. Most ordered...
Controlling the aggregation of vital bacterial proteins could be one new research directions and form basis for search development antibacterial drugs with targeted action. Such approach may considered as an alternative to antibiotics. Amyloidogenic regions can, like peptides, interact “parent” protein, example, ribosomal S1 protein (specific only bacteria), interfere its functioning. The aim work was peptides based on from T. thermophilus, exhibiting both properties. biological system...
The data presented in this article are related to the research entitled "One of possible mechanisms amyloid fibrils formation based on sizes primary and secondary folding nuclei Aβ40 Aβ42" (Dovidchenko et al., 2016) [1]. Aβ peptide is one most intensively studied amyloidogenic peptides. Despite huge number articles devoted studying different fragments there only several papers with correct kinetics data, also a few X-ray especially for Aβ42. Our present diffraction patterns both Aβ42 as well...
The main function of dUTPases is to regulate the cellular levels dUTP and dTTP, thereby playing a crucial role in DNA repair mechanisms. Despite fact that mutant organisms with obliterated dUTPase enzymatic activity remain viable, it not possible completely knock out dut gene due lethal consequences such mutation for organism. As result, considered this class enzymes performs an additional essential organism's survival. In study, we provide evidence bacteriophage T5 fulfills supplemental...
Bacterial S1 protein is a functionally important ribosomal protein. It part of the 30S subunit and also able to interact with mRNA tmRNA. An feature family strong tendency towards aggregation. To study amyloidogenic properties S1, we isolated purified recombinant Pseudomonas aeruginosa. Using FoldAmyloid, Waltz, Pasta 2.0, AGGRESCAN programs, regions were predicted, which play key role in its The method limited proteolysis combination high performance liquid chromatography mass spectrometric...
The main function of dUTPases is to regulate the cellular levels dUTP and dTTP, thereby playing a crucial role in DNA repair mechanisms. Despite fact that mutant organisms with obliterated dUTPase enzymatic activity remain viable, it not possible knockout dut gene completely due lethal consequences such mutation for organism. As result, considered this class enzymes perform an additional essential organism's survival. In study, we provide evidence bacteriophage T5 possesses...
The study of protein enzymatic activities has always been a significant area scientific and industrial research. key stages typically undertaken in the characterization given enzyme family include establishing mechanism catalysis, measuring kinetic parameters, determining structural organization architecture catalytic center, subsequential classification. In this review, we address these classical aspects concerning dUTPase family, specifically focusing on spatial structure activity, while...
An extensive study of the folding and stability proteins their complexes has revealed a number problems questions that need to be answered. One them is effect chaperones on process fibrillation various peptides.We studied molecular chaperones, such as GroEL α-crystallin, fibrillogenesis Aβ(1-42) peptide using electron microscopy surface plasmon resonance.Recombinant were isolated purified. It was shown assembly occurs without addition magnesium potassium ions, commonly believed. According...