A5008533997- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Enzyme-mediated dye degradation
- Biochemical and Molecular Research
- RNA modifications and cancer
- Enzyme Production and Characterization
- Microbial Metabolism and Applications
- Spectroscopy and Quantum Chemical Studies
- Bacterial Genetics and Biotechnology
- Metal-Catalyzed Oxygenation Mechanisms
- melanin and skin pigmentation
- Photoreceptor and optogenetics research
- Algal biology and biofuel production
- Protein Structure and Dynamics
- Cancer Research and Treatments
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Porphyrin Metabolism and Disorders
- Biofuel production and bioconversion
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Folate and B Vitamins Research
- Microbial Metabolites in Food Biotechnology
- Toxin Mechanisms and Immunotoxins
Institute of Protein Research
2015-2024
Russian Academy of Sciences
2010-2021
A.V. Shubnikov Institute of Crystallography
2006-2021
Federal Scientific Research Centre Crystallography and Photonics
2016-2021
Kazan Federal University
2020
Institute Belniis
2018
Freie Universität Berlin
2009-2011
University of Pavia
2010
Diamond Light Source
2009
Abstract We report the crystal structure of a 40mer mirror-image RNA oligonucleotide completely built from nucleotides non-natural L -chirality in complex with pro-inflammatory chemokine -CLL2 (monocyte chemoattractant protein-1), natural protein composed regular -amino acids. The -oligonucleotide is an -aptamer (a Spiegelmer) identified to bind -CCL2 high affinity, thereby neutralizing chemokine’s activity. CCL2 plays key role attracting and positioning monocytes; its overexpression several...
Herbicides that target photosystem II (PSII) compete with the native electron acceptor plastoquinone for binding at Q(B) site in D1 subunit and thus block transfer from Q(A) to Q(B). Here, we present first crystal structure of PSII a bound herbicide resolution 3.2 Å. The crystallized core complexes were isolated thermophilic cyanobacterium Thermosynechococcus elongatus. used terbutryn is found bind via least two hydrogen bonds similar photosynthetic reaction centers anoxygenic purple...
The membrane-embedded photosystem II core complex (PSIIcc) uses light energy to oxidize water in photosynthesis. Information about the spatial structure of PSIIcc obtained from x-ray crystallography was so far derived homodimeric thermophilic cyanobacteria. Here, we report first crystallization and structural analysis monomeric form with high oxygen evolution capacity, isolated Thermosynechococcus elongatus. crystals belong space group C222(1), contain one monomer per asymmetric unit,...
Abstract For the sake of energy preservation, bacteria, upon transition to stationary phase, tone down their protein synthesis. This process is favored by reversible binding small stress-induced proteins ribosome prevent unnecessary translation. One example conserved bacterial silencing factor (RsfS) that binds uL14 onto large ribosomal subunit and prevents its association with subunit. Here we describe mode Staphylococcus aureus RsfS present a 3.2 Å resolution cryo-EM reconstruction...
The crystal structure of the myotoxic, cell-penetrating, basic polypeptide crotamine isolated from venom Crotalus durissus terrificus has been determined by single-wavelength anomalous dispersion techniques and refined at 1.7 Å resolution. reveals distinct cationic hydrophobic surface regions that are located on opposite sides molecule. This surface-charge distribution indicates its possible mode interaction with negatively charged phospholipids other molecular targets to account for diverse...
Abstract Sleeping sickness is a fatal disease caused by the protozoan parasite Trypanosoma brucei (Tb). Inosine-5’-monophosphate dehydrogenase (IMPDH) has been proposed as potential drug target, since it maintains balance between guanylate deoxynucleotide and ribonucleotide levels that pivotal for parasite. Here we report structure of TbIMPDH at room temperature utilizing free-electron laser radiation on crystals grown in living insect cells. The 2.80 Å resolution reveals presence ATP GMP...
The structure of the large ribosomal subunit from halophilic archaeon Haloarcula marismortui (Hma) is only crystal an archaeal particle that has been determined to date. However, first model Hma 50S contained some gaps: structures functionally important mobile lateral protuberances were not visualized. Subsequently, parts P (L12) stalk base visualized at 3.0 Å resolution [Kavran & Steitz (2007), J. Mol. Biol. 371, 1047–1059]: RNA-binding domain r-protein P0 (L10), C-terminal L11 and helices...
Laccases (EC 1.10.3.2) are multicopper oxidoreductases acting on diphenols and related substances. highly important for biotechnology environmental remediation. These enzymes contain mononuclear one T2 copper ion two T3 ions (Cu3α Cu3β), which form the so-called trinuclear center (TNC). Along with typical three-domain laccases Bacteria produce two-domain (2D) enzymes, active at neutral basic pH, thermostable, resistant to inhibitors. In this work we present comparative analysis of crystal...
The crystal structure of the isolated full-length ribosomal L1 stalk, consisting Thermus thermophilus protein in complex with a specific 80-nucleotide fragment 23S rRNA, has been solved for first time at high resolution. revealed details protein–RNA interactions stalk. Analysis packing enabled identification sticky sites on and rRNA which may be important ribosome assembly function. was used to model different conformational states ribosome. This approach provides an insight into roles...
As a basis of photosynthesis, photoinduced oxidation (bacterio)chlorophyll molecules in the special reaction center complexes has been subject extensive research. In contrast, generally harmful photooxidation antenna chromoproteins received much less attention. Here, we have established permanent structural changes LH2 bacteriochlorophyll-protein complex from sulfur photosynthetic purple bacterium Ectothiorhodospira haloalkaliphila taking place at physiological conditions upon intense...
Laccases catalyze the oxidation of substrates with concomitant reduction oxygen to water. Recently, we found that polar residues located in tunnels leading Cu2 and Cu3 ions control entrance (His 165) proton transport (Arg 240) two-domain laccase (2D) from Streptomyces griseoflavus (SgfSL). In this work, have focused on optimizing substrate-binding pocket (SBP) SgfSL while simultaneously adjusting process. variants three single (Met199Ala, Met199Gly, Tyr230Ala) double amino acid substitutions...
The bacterial Sm-like protein Hfq forms homohexamers both in solution and crystals. monomers are organized as a continuous beta-sheet passing through the whole hexamer ring with common hydrophobic core. Analysis of Pseudomonas aeruginosa (PaeHfq) structure suggested that solvent-inaccessible intermonomer hydrogen bonds created by conserved amino-acid residues should also stabilize quaternary protein. In this work, one such residue, His57, PaeHfq was replaced alanine, threonine or asparagine....
Ribosome biogenesis is a complex and highly accurate conservative process of ribosomal subunit maturation followed by association. Subunit comprises sequential stages RNA proteins' folding, modification binding, with the involvement numerous RNAses, helicases, GTPases, chaperones, RNA, protein-modifying enzymes, assembly factors. One such factor involved in bacterial 30S binding A (RbfA). In this study, we present crystal (determined at 2.2 Å resolution) NMR structures RbfA as well 2.9...
Staphylococcus aureus is a bacterial pathogen and one of the leading causes healthcare-acquired infections in world. The growing antibiotic resistance S. obliges us to search for new drugs treatments. As majority antibiotics target ribosome, knowledge its detailed structure crucial drug development. Here, we report cryo-EM reconstruction at 3.2 Å resolution ribosome with P-site tRNA, messenger RNA, 10 RNA modification sites previously not assigned or visualized. resulting model most precise...
A giant multidomain protein of striated and smooth vertebrate muscles, titin, consists tandems immunoglobulin (Ig)- fibronectin type III (FnIII)-like domains representing β-sandwiches, as well disordered segments. Chicken muscles express several titin isoforms ~500-1500 kDa. Using various structural-analysis methods, we investigated in vitro nonspecific amyloid aggregation the high-molecular-weight isoform chicken smooth-muscle (SMTHMW, ~1500 kDa). As confirmed by X-ray diffraction analysis,...