A5078813273- Parasitic Diseases Research and Treatment
- Parasite Biology and Host Interactions
- Insect symbiosis and bacterial influences
- Parasites and Host Interactions
- Insects and Parasite Interactions
- Nematode management and characterization studies
- Helminth infection and control
- Chemokine receptors and signaling
- Horticultural and Viticultural Research
- Bat Biology and Ecology Studies
- Immune Response and Inflammation
- Fermentation and Sensory Analysis
- Identification and Quantification in Food
- Parasitic infections in humans and animals
- Mollusks and Parasites Studies
- Lymphatic System and Diseases
- Insect and Pesticide Research
- Asthma and respiratory diseases
- Planarian Biology and Electrostimulation
- Insect and Arachnid Ecology and Behavior
- Evolution and Paleontology Studies
- Sleep and related disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Species Distribution and Climate Change
- Forensic Entomology and Diptera Studies
Molécules de Communication et Adaptation des Micro-organismes
2016-2025
Centre National de la Recherche Scientifique
2014-2025
Sorbonne Université
2015-2025
Muséum national d'Histoire naturelle
2015-2024
Institut des Sciences Moléculaires
2015
Institut de Systématique, Évolution, Biodiversité
2000-2013
Université Grenoble Alpes
2013
Structure et Instabilité des Génomes
2009-2012
University Hospital Bonn
2012
Inserm
2002-2010
We compared here the suitability and efficacy of traditional morphological approach DNA barcoding to distinguish filarioid nematodes species (Nematoda, Spirurida). A reliable rapid taxonomic identification these parasites is basis for a correct diagnosis important widespread parasitic diseases. The performance with different parameters was measuring strength correlation between molecular approaches. Molecular distance estimation performed two mitochondrial markers (coxI 12S rDNA)...
Background Wolbachia are intriguing symbiotic endobacteria with a peculiar host range that includes arthropods and single nematode family, the Onchocercidae encompassing agents of filariases. This raises question origin infection in filariae. infect female germline hypodermis. Some evidences lead to theory act as mutualist coevolved filariae from one event: their removal sterilizes filariae; all specimens positive species infected; vertically inherited; few lost symbiont. However, most data...
During the past twenty years, a number of molecular analyses have been performed to determine evolutionary relationships Onchocercidae, family filarial nematodes encompassing several species medical or veterinary importance. However, opportunities for broad taxonomic sampling scarce, and were based mainly on 12S rDNA coxI gene sequences. While being suitable differentiation, these mitochondrial genes cannot be used infer phylogenetic hypotheses at higher levels. In present study, 48 species,...
Wolbachia is an alpha-proteobacterial symbiont widely distributed in arthropods. Since the identification of certain animal-parasitic nematodes (the Onchocercidae or filariae), relationship between arthropod and nematode has attracted great interest. The obligate symbiosis filariae, which renders infected species susceptible to antibiotic chemotherapy, was held be distinct from Wolbachia-arthropod relationship, typified by reproductive parasitism. While co-evolutionary signatures symbioses...
Endothelial progenitor cell (EPC) transplantation has beneficial effects for therapeutic neovascularization; however, only a small proportion of injected cells home to the lesion and incorporate into neocapillaries. Consequently, this type therapy requires substantial improvement be clinical value. Erythropoietin-producing human hepatocellular carcinoma (Eph) receptors their ephrin ligands are key regulators vascular development. We postulated that activation EphB4/ephrin-B2 system may...
Parasitic filarial nematodes that belong to the Onchocercidae family live in mutualism with Wolbachia endosymbionts. We developed whole-mount techniques follow segregation patterns of through somatic and germline lineages four species. These studies reveal multiple evolutionarily conserved mechanisms are required for localization germline. During initial embryonic divisions, segregate asymmetrically such they concentrate posteriorly localized P(2) blastomere, a precursor adult hypodermal...
Eosinophils mediate protection against filarial nematodes. Our results demonstrate that eosinophil extracellular traps (EETosis) are induced by microfilariae and infective L3 larvae of Litomosoides sigmodontis. These DNA inhibit motility in a DNA- contact-dependent manner vitro. Accordingly, microfilariae-injection triggers release an eosinophil-dependent vivo covered with cleared more rapidly. Using dectin-1, we identify the required receptor for microfilariae-induced EETosis, whereas...
Summary An increase in circulating neutrophils is a characteristic feature of many inflammatory reactions and result the rapid mobilization from bone marrow, driven by mediators, including ELR + CXC chemokines. In this paper, using combination light electron microscopy an situ perfusion system rat femoral we examined process detail. We show that stimulated chemokine, MIP‐2, involves neutrophil migration haematopoietic compartment marrow across sinusoidal endothelium via transcellular route....
Onchocerca ochengi , a filarial parasite of cattle, represents the closest relative human pathogen, volvulus . Both species harbour Wolbachia endosymbionts and are remarkable in that adult female worms remain viable but sessile for many years while surrounded by host cells antibodies. The basis symbiosis between filariae is thought to be metabolic, although role immune evasion has received little attention. Neutrophils attracted following antibiotic chemotherapy they replaced eosinophils...
Wolbachia are alpha-proteobacteria symbionts infecting a large range of arthropod species and two different families nematodes. Interestingly, these endosymbionts able to induce diverse phenotypes in their hosts: they reproductive parasites within many arthropods, nutritional mutualists some insects obligate filarial nematode hosts. Defining ‘species’ is controversial so commonly classified into 17 phylogenetic lineages, termed supergroups, named A–F, H–Q S. However, available genomic data...
A major impediment to eliminate lymphatic filariasis and onchocerciasis is the lack of effective short-course macrofilaricidal drugs or regimens that are proven be safe for both infections. In this study we tested oxfendazole, an anthelmintic shown well tolerated in phase 1 clinical trials. vitro, oxfendazole exhibited modest marginal motility inhibition adult worms Onchocerca gutturosa, pre-adult volvulus lienalis microfilariae. vivo, five days oral treatments provided sterile cure with up...
ABSTRACT In order to establish the role of eosinophils in destroying parasites, transgenic mice have been used experimental helminthiases but not filariasis. Litomosoides sigmodontis offers a good opportunity for this study because it is only filarial species that completes its life cycle mice. Its development was compared CBA/Ca overexpressing interleukin-5 (IL-5) and wild-type following subcutaneous inoculation 40 infective larvae. An acceleration larval growth observed IL-5 However,...
Onchocerciasis, caused by the filarial nematode Onchocerca volvulus, is a parasitic disease leading to debilitating skin and blindness, with major economic social consequences. The pathology of onchocerciasis principally considered be consequence long-standing host inflammatory responses. In subcutaneous nodule formed around female worms, core which dense infiltrate cells in microfilariae are released. It has been established that formation nodules associated angiogenesis. this study, we...
Abstract Filarial parasites have to trespass many barriers successfully settle within their mammalian host, which is equipped with mechanical borders and complex weaponry of an evolved immune system. However, little known about mechanisms early local events in filarial infections. In this study, bone marrow-derived dendritic cells not only upregulated activation markers CD40 CD80 upon vitro stimulation extracts, but also secreted CCL17, a chemokine be produced microbial challenge. Mice...
Three species of the genus Cercopithifilaria have been morphologically and molecularly characterized in dog populations southern Europe: grassii (Noè, 1907), sp. sensu Otranto et al., 2011 (reported as I), II 2012. The adults I remained unknown until present study. material originated from a Sardinia (Italy) diagnosed with dermal microfilariae I. holotype three paratypes bainae Almeida & Vicente, 1984, described dogs Brazil, were studied comparative material. A cox 1 (~689 bp) 12S (~330 gene...