A5029103273- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Cancer-related Molecular Pathways
- Immunotherapy and Immune Responses
- Axon Guidance and Neuronal Signaling
- Advanced biosensing and bioanalysis techniques
- Melanoma and MAPK Pathways
- Inflammatory Bowel Disease
- Extracellular vesicles in disease
- Digital Holography and Microscopy
- Skin Protection and Aging
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- Immune Cell Function and Interaction
- Protein Kinase Regulation and GTPase Signaling
- Cytokine Signaling Pathways and Interactions
- Platelet Disorders and Treatments
- Microtubule and mitosis dynamics
- Thermal Regulation in Medicine
- Proteoglycans and glycosaminoglycans research
- Plant tissue culture and regeneration
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2013-2024
CEA Grenoble
2013-2024
Inserm
2012-2024
Université Grenoble Alpes
2014-2024
Laboratoire Biosciences et bioingénierie pour la Santé
2018-2024
Lebanese University
2023
Centre Hospitalier Universitaire de Bordeaux
2019
Institut de Recherches en Technologies et Sciences pour le Vivant
2010-2015
Laboratoire Biologie à Grande Échelle
2012-2015
Université Joseph Fourier
2012
Cardiac glycosides kill cancer cells in a way that stimulates the immune response.
Background information . Endothelial cells play a major role in angiogenesis, the process by which new blood vessels arise from pre‐existing vascular bed. VEGF‐A (vascular endothelial growth factor‐A) is key regulator of angiogenesis during both development and adults. HGF (hepatocyte factor) pleiotropic cytokine that may promote VEGF‐A‐driven although signalling mechanisms underlying this co‐operation are not completely understood. Results We analysed effects combination on activation...
Endothelial progenitor cell (EPC) transplantation has beneficial effects for therapeutic neovascularization; however, only a small proportion of injected cells home to the lesion and incorporate into neocapillaries. Consequently, this type therapy requires substantial improvement be clinical value. Erythropoietin-producing human hepatocellular carcinoma (Eph) receptors their ephrin ligands are key regulators vascular development. We postulated that activation EphB4/ephrin-B2 system may...
Sumoylation during genotoxic stress regulates the composition of DNA repair complexes. The yeast metalloprotease Wss1 clears chromatin-bound sumoylated proteins. and its mammalian analog, DVC1/Spartan, belong to minigluzincins family proteases. proteolytic activity is regulated by a cysteine switch mechanism activated chemical and/or binding. required for cell survival following UV irradiation, smt3-331 mutation Camptothecin-induced formation covalent topoisomerase 1 complexes (Top1cc)....
Androgen receptor (AR) signalling is a key prostate cancer (PC) driver, even in advanced 'castrate-resistant' disease (CRPC). To systematically identify microRNAs (miRs) modulating AR activity lethal disease, hormone-responsive and -resistant PC cells expressing luciferase-based reporter were transfected with miR inhibitor library; 78 inhibitors significantly altered activity. Upon validation, miR-346, miR-361-3p miR-197 markedly reduced transcriptional activity, mRNA protein levels,...
Quantification of basic cell functions is a preliminary step to understand complex cellular mechanisms, for e.g., test compatibility biomaterials, assess the effectiveness drugs and siRNAs, control behavior. However, commonly used quantification methods are label-dependent, end-point assays. As an alternative, using our lensfree video microscopy platform perform high-throughput real-time monitoring culture, we introduce specifically devised metrics that capable non-invasive such as...
The p63 protein plays a key role in regulating human keratinocyte proliferation and differentiation. Although some p63-regulating microRNAs (miRNAs) have been identified the control of epidermal homeostasis, little is known about miRNAs acting downstream p63. In this paper, we characterized multiple p63-regulated (miR-17, miR-20b, miR-30a, miR-106a, miR-143 miR-455-3p) elucidated their roles onset We RB, p21 MAPKs as targets these p63-controlled miRNAs. Upon inhibition most miRNAs, observed...
Although cadherins appear to be necessary for proper cell-cell contacts, the physiological role of VE-cadherin (vascular endothelium cadherin) in adult tissue has not been clearly determined. To shed some light on this question, we have disturbed adhesive function human endothelial cell culture using a polyclonal anti-VE-cadherin antibody. This antibody disrupts confluent monolayers <i>in vitro</i> and transiently generates numerous gaps at junctions. The formation these correlates with...
Abstract MiRNAs are key regulators of gene expression. By binding to many genes, they create a complex network co-regulation. Here, using network-based approach, we identified miRNA hub groups by their close connections and common targets. In one cluster containing three miRNAs, miR-612, miR-661 miR-940, the annotated functions co-regulated genes suggested role in small GTPase signalling. Although members this targeted same subset predicted showed that overexpression impacted cell fates...
Complex in vitro models of human immune cells and intestinal mucosa may have a translation-assisting role the assessment anti-inflammatory compounds. Chronic inflammation gastrointestinal tract is hallmark inflammatory bowel diseases (IBD). In both IBD entities, Crohn's disease ulcerative colitis, impaired cell activation dysfunctional epithelial barrier are common pathophysiology. Current therapeutic approaches targeting single modulator molecules to stop progression reduce adverse effects....
Inflammatory neovascularization, such as choroidal neovascularization (CNV), occur in the presence of Notch expressing macrophages. DLL4s anti-angiogenic effect on endothelial cells (EC) has been widely recognized, but its influence signaling macrophages and overall inflammatory is not well understood. We identified ECs main 1 4 CNV. A soluble fraction spanning Ser28-Pro525 murine extracellular DLL4 domain (sDLL4/28-525) activated pathway, it induces target genes inhibited EC proliferation...
Breast cancer (BC) is the most diagnosed in women worldwide. In estrogen receptor (ER)-positive disease, anti-estrogens and aromatase inhibitors (AI) improve patient survival; however, many patients develop resistance. Dysregulation of apoptosis a common resistance mechanism; thus, agents that can reinstate activity apoptotic pathways represent promising therapeutics for advanced drug-resistant disease. Emerging targets this scenario include microRNAs (miRs). To identify miRs modulating...
Xeroderma pigmentosum group C (XPC) is a versatile protein crucial for sensing DNA damage in the global genome nucleotide excision repair (GG-NER) pathway. This pathway vital mammalian cells, acting as their essential approach repairing lesions stemming from interactions with environmental factors, such exposure to ultraviolet (UV) radiation sun. Loss-of-function mutations XPC gene confer photosensitive phenotype XP-C patients, resulting accumulation of unrepaired UV-induced damage....
Abstract Inhibition of protein degradation by blocking Cullin-RING E3 ligases (CRLs) is a new approach in cancer therapy though unknown risk because CRL inhibition may stabilize both oncoproteins and tumor suppressors. Probing CRLs prostate cells revealed remarkable plasticity with TMPRSS2-ERG translocation. suppression chemical or knockdown RING component RBX1 led to reversible G0/G1 cell cycle arrest that prevented apoptosis. Conversely, complete at higher inhibitor dose-induced...
The behaviour of cancerous epithelial prostatic cells (PC3) growing on polyelectrolytes (PE) coatings was compared to the immortalized normal (PNT-2). cell evaluated and quantified in terms initial attachment, growth, metabolic activity, morphometry, adhesion, apoptosis stress related gene expression. Both anionic PSS (poly(sodium 4-styrenesulphonate))-terminated surface cationic PAH (poly(allylamine hydrochloride))-terminated surfaces were not cytotoxic. attachment better PAH-terminated...