A5024225433- Endodontics and Root Canal Treatments
- Dental materials and restorations
- Dental Radiography and Imaging
- Wound Healing and Treatments
- Bone and Dental Protein Studies
- Bone Tissue Engineering Materials
- Inflammatory mediators and NSAID effects
- Mesenchymal stem cell research
- Dental Implant Techniques and Outcomes
- Surgical Sutures and Adhesives
- Phagocytosis and Immune Regulation
- Cell Adhesion Molecules Research
- Alkaline Phosphatase Research Studies
- Bone health and treatments
- Oral microbiology and periodontitis research
- Immune cells in cancer
- Drug Transport and Resistance Mechanisms
- Protease and Inhibitor Mechanisms
- Vitamin C and Antioxidants Research
- Periodontal Regeneration and Treatments
- Connective tissue disorders research
- Pain Mechanisms and Treatments
- Oral and Maxillofacial Pathology
- Heterotopic Ossification and Related Conditions
- Oral health in cancer treatment
Niigata University
2015-2025
University of Michigan
2023
Takenaka (Japan)
2022
Matsumoto Dental University
2015
Regenerative endodontic procedures using blood clots (BC-REP) for immature teeth typically exhibit a periodontal ligament–like healing pattern. However, pulp-like pattern is observed in the presence of residual pulp. This study aimed to clarify phenotype according tooth maturity when performing BC-REP pulp, focusing on migrated mesenchymal stem/stromal cells (MSCs). rat molar models were created pulp at ages corresponding developmental stages, from mature (5 wk: immature, root still growing;...
This study assessed the biocompatibility and chemical properties of two bioceramic root canal sealers, EndoSequence BC Sealer (EBC) Nishika Canal BG (NBG), using a sealer extrusion model. Eight-week-old male Wistar rats were used. The mesial canals upper first molars pulpectomized overfilled with EBC, NBG, or, as reference, epoxy resin-based AH Plus (AHP). After 28 days, periapical tissue reactions microcomputed tomography histological staining. elemental composition extruded EBC NBG...
Macrophages are immune cells with high plasticity that perform many functions related to tissue injury and repair. They generally categorized as 2 functional phenotypes: M1 (proinflammatory) M2 (anti-inflammatory prohealing). To investigate the role of macrophages in human dental pulp, we examined localization distributional alterations healthy pulp well during reparative process capping mineral trioxide aggregate (MTA) cariously inflamed adult teeth. We also quantified populations M1/M2 by...
Calcium salt precipitation induced by intracanal medicaments contributes to the formation of apical hard tissue during apexification. This study compared calcium salt-forming ability a new silicate-based medicament (Bio-C Temp) with that two commercial hydroxide pastes (Calcipex Plane II and Vitapex) in rat subcutaneous implantation model. Polytetrafluoroethylene tubes containing each three materials were subcutaneously implanted 4-week-old male Wistar rats. After 28 days, composition amount...
Ascorbic acid (AA; vitamin C) plays a crucial role in the biosynthesis and secretion of collagen to produce organic matrix hard tissues. Nevertheless, detailed mechanism by which AA induces reparative dentinogenesis is still unknown. This study aimed investigate pathway function during wound healing rat pulpotomy model. Sodium-dependent C transporter (SVCT) 2 glucose (GLUT) 1 were detected odontoblasts, endothelial cells, nerve fibers normal pulp SVCT2 GLUT1 also expressed odontoblast-like...
Hydroxyapatite formation on endodontic hydraulic calcium silicate cements (HCSCs) plays a significant role in sealing the root canal system and elevating hard-tissue inductivity of materials. This study evaluated vivo apatite-forming ability 13 new-generation HCSCs using an original HCSC (white ProRoot MTA: PR) as positive control. The were loaded into polytetrafluoroethylene tubes implanted subcutaneous tissue 4-week-old male Wistar rats. At 28 days after implantation, hydroxyapatite...
Abstract Hydroxyapatite formation on endodontic hydraulic calcium silicate cements (HCSCs) plays a significant role in sealing the root canal system and elevating hard-tissue inductivity of materials. This study aimed to evaluate vivo apatite-forming ability 13 second-generation HCSCs using representative first-generation HCSC (white ProRoot MTA: PR) as positive control. Thirteen PR were loaded into polytetrafluoroethylene tubes implanted subcutaneous tissue 4-week-old male Wistar rats. At...
Mineral trioxide aggregate (MTA) is a commonly used dental pulp-capping material with known effects in promoting reparative dentinogenesis. However, the mechanism by which MTA induces dentine repair remains unclear. The aim of present study was to investigate role prostaglandin E2 (PGE2) examining localisation and mRNA expression levels its transporter (Pgt) two receptors (Ep2 Ep4) rat model pulpotomy capping. Ep2 detected odontoblasts, endothelial cells, nerve fibres normal pulpotomised...
Myofibroblasts and extracellular matrix are important components in wound healing. Alpha-smooth muscle actin (α-SMA) is a marker of myofibroblasts. Fibrillin-1 major constituent microfibrils an extracellular-regulator TGF-β1, cytokine the transdifferentiation resident fibroblasts into To study correlation between changes fibrillin-1 expression myofibroblast differentiation, we examined alterations α-SMA organotypic cultures dental pulp vitro. Extracted healthy human teeth were cut to...
Abstract When regenerative endodontic procedures (REPs) are performed on immature teeth diagnosed with pulp necrosis and apical periodontitis, various healing patterns occur. Furthermore, infected disorders often exhibit some remnant tissue. Therefore, this study investigated the impact of healthy or fully functional tissue after REPs. Simulated REPs were non-infected rat molars different amounts Healing in these assessed 28 days. Teeth 0.81–0.91 mm healed pulp-like tissue, dentin,...
Concentrated growth factor (CGF) is a new-generation autologous platelet concentrate that promotes tissue regeneration and has anti-inflammatory properties. This randomized multicenter trial aimed to evaluate the effects of CGF on bone healing in combination with root-end microsurgery. Healthy adult patients indicated for microsurgery were randomly assigned either or control (no implantation) groups. was implanted into cavity after filling mineral trioxide aggregate. Clinical periapical...
GaAlAs lasers induce pulp mineralization by promoting reparative dentinogenesis. This study analyzed the expression of dentin matrix protein 1 (DMP1) and osteopontin in laser-irradiated rat molars, to examine hypothesis that these proteins play a role laser-induced dentinogenic process.The mesial surfaces upper first molars 8-week-old Wistar rats were irradiated with pulsed laser. After 1-14 days, mRNA DMP1 coronal was using real-time PCR. DMP1, osteopontin, heat shock 25 (HSP25)...
To explore the expression profile of CD45+/pro-collagen I+ fibrocytes in intact dental pulps as well during wound healing adult pulp tissue.A total 16 healthy permanent teeth were obtained from young patients (18 to 25 years) undergoing orthodontic treatment. Routine capping with mineral trioxide aggregate (MTA) was performed under local anaesthesia induce a mineralized barrier at exposed surface. Teeth extracted after 7, 14 and 35 days. Sections prepared stained for various markers using...
To determine glucose transporter 1 (GLUT1) and runt-related transcription factor 2 (RUNX2) expression during reparative dentinogenesis after pulpotomy with mineral trioxide aggregate (MTA) capping.Eight-week-old male Wistar rats were used. Pulp of the upper left first molar was exposed capped MTA. The right same animal used as a control. After collecting molars at various time points, GLUT1, RUNX2 mammalian target rapamycin (MTOR) examined by immunohistochemistry. mRNA levels Slc2a1...
Resin monomers and polymerisation initiators have been shown to be cytotoxic for pulp cells disturb odontoblast differentiation. This study aimed compare the effect of a resin-modified calcium silicate cement (TheraCal LC; TC) resin-free (ProRoot MTA; PR) on pulpal healing after pulpotomy. Pulpotomy was performed maxillary first molars 8-week-old rats using either PR or TC. After 1, 3, 7, 14 28 days, responses were assessed by micro-computed tomography, haematoxylin-eosin staining...