A5058943535- Chronic Myeloid Leukemia Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Hemoglobinopathies and Related Disorders
- Cytokine Signaling Pathways and Interactions
- Iron Metabolism and Disorders
- SARS-CoV-2 and COVID-19 Research
- Eosinophilic Disorders and Syndromes
- Acute Lymphoblastic Leukemia research
- Histone Deacetylase Inhibitors Research
- Machine Learning in Bioinformatics
- RNA modifications and cancer
- Lipid Membrane Structure and Behavior
- Saffron Plant Research Studies
- Plant tissue culture and regeneration
- Kruppel-like factors research
- Caveolin-1 and cellular processes
- Cancer-related gene regulation
- Pancreatic function and diabetes
- Cellular transport and secretion
- Botanical Research and Applications
- vaccines and immunoinformatics approaches
- Epigenetics and DNA Methylation
- Hematological disorders and diagnostics
- COVID-19 Clinical Research Studies
Hospital Universiti Sains Malaysia
2018-2024
Hospital Raja Perempuan Zainab II
2024
Universiti Sains Malaysia
2014-2023
Sultan Zainal Abidin University
2020
Tumor-suppressor genes are inactivated by methylation in several cancers including acute myeloid leukemia (AML). Src homology-2 (SH2)-containing protein-tyrosine phosphatase 1 (SHP-1) is a negative regulator of the JAK/STAT pathway. Transcriptional silencing SHP-1 plays critical role development and progression through STAT3 activation. 5-Azacytidine (5-Aza) DNA methyltransferase inhibitor that causes demethylation resulting re-expression silenced SHP-1. Lestaurtinib (CEP-701) multi-targeted...
Silencing due to methylation of suppressor cytokine signaling-3 (SOCS-3), a negative regulator gene for the JAK/STAT signaling pathway has been reported play important roles in leukemogenesis. Imatinib mesylate is tyrosine kinase inhibitor that specifically targets BCR-ABL protein and induces hematological remission patients with chronic myeloid leukemia (CML). Unfortunately, majority CML treated imatinib develop resistance under prolonged therapy. We here investigated profile SOCS-3 its...
Background: Pereskia sacharosa is a genus of cacti widely used in folk medicine for cancer-related treatment. Anti-proliferative effects have been studied recent years against colon, breast, cervical and lung cancer cell lines, with promising results. We here extended study anti-proliferative to blood malignancy, leukemia. Materials Methods: Two leukemic MV4-11 (acute myeloid leukemia) K562 (chronic leukemia), were studied. <TEX>$IC_{50}$</TEX> concentrations determined apoptosis cycle...
Background: Epigenetic silencing of tumor suppressor genes (TSG) is involved in development and progression cancers. Re-expression TSG inversely proportionate with STAT3 signaling pathways. Demethylation DNA by 5-Azacytidine (5-Aza) results re-expression silenced TSG. Forced expression PRG2 5-Aza induced apoptosis cancer cells. Imatinib a tyrosine kinase inhibitor that potently inhibits BCR/ ABL resulting hematological remission CML patients. However, majority patients treated imatinib would...
Background: Imatinib mesylate, a tyrosine kinase inhibitor specifically targeting the BCR/ABL fusion protein, induces hematological remission in patients with chronic myeloid leukemia (CML). However, majority of CML treated imatinib develop resistance prolonged therapy. Dendrophthoe pentandra (L.) Miq. is Malaysian mistletoe species that has been used as traditional treatment for several ailments such smallpox, ulcers, and cancers. Methods: We developed resistant cell line (designated K562R)...
Background: Acute myeloid leukemia (AML) is the most common form of acute leukemias in adults which clinically and molecularly heterogeneous. Several risk genetic factors have been widely investigated to characterize AML. However, concomitant epigenetic controlling gene expression lead AML transformation was not fully understood. This study aimed identify epigenetically regulated genes cell lines induced by modulating agents, Trichostatin A (TSA) 5-Azacytidine (5-Aza).
 Materials...
Background Hb Adana is a non-deletional alpha (α)-thalassaemia variant resulting from mutations in α1- or α2-globin codon 59 (αCD59), leading to the production of unstable α-globin. Clinical manifestations can vary silent carrier status dependence on blood transfusions, hepatosplenomegaly, skeletal deformities, and spinal cord compression. Despite significance inheritance, studying this poses challenges due scarcity molecular tests potential for routine diagnoses be overlooked. This study...
Background and objective Suppressor of cytokine signaling-3 (SOCS-3) has been shown to be an important candidate in molecular therapeutic strategies management acute myeloid leukemia (AML), particularly patients carrying FLT3-ITD mutation. SOCS-3 suppresses signalling by inhibiting the activity Janus Kinase-2 (JAK-2), competing with signal transducer activator transcription (STAT) molecules that leads underexpression. The study aims determine epigenetically silence genes AML cells a...
Hb Adana is a non-deletional α-thalassaemia variant, due to mutations in α1- or α2-globin codon 59 (αCD59) that produce unstable α-globin. Clinical appearance can range from silent carrier blood transfusion reliance, hepatosplenomegaly, skeletal deformities, and spinal cord compression. Despite the importance of inheritance, this variant challenging study since molecular tests are scarce routine diagnosis be missed. This explored distribution among local high school students evaluate...
Biologically, Acute myeloid leukemia (AML) is highly heterogenous. AML with cup-like blast morphology variant has been reported to have important role in risk group stratification and treatment implications. In pediatric age group, this its clinical implication rarely discussed. Although not stated World Health Organization (WHO) classification of Tumours Haematopoietic Lymphoid Tissues, it associated poor outcome from the association other features. A 10- year old girl diagnosed study. Her...
Calreticulin (CALR) mutations in myeloproliferative neoplasms (MPN) have been reported to be key markers the molecular diagnosis, particularly patients lacking JAK2 V617F mutation. In most current reports, CALR were analysed by either allele-specific PCR (AS-PCR), or more expensive quantitative real-time PCR, pyrosequencing and next-generation sequencing. Hence, we report use of an alternative method, conformation sensitive gel electrophoresis (CSGE) for detection BCR-ABL1-negative MPN...
The novel coronavirus 2019 (COVID-19) has struck more than 99 million people worldwide and had claimed 2 lives as of 23 January 2021, which affecting 221 countries/nations. Until now, the pandemic not shown signals slowing down, with no proven vaccine in sight. People are speculating on this unprecedented event. It is well documented that receptor-binding domain (RBD) viral spiked S1 glycoprotein directly bind angiotensin-converting enzyme (ACE2) dipeptidyl-peptidase-4 (DPP4) or CD26...
COVID-19 caused by the SARS-CoV-2 virus has become a real threat due to emergence of new variants which are more deadly with higher infectivity. Vaccine constructs that target specific needed for stemming fatality. The spike (S) glycoprotein is major antigenic component triggers host immune response. Reverse vaccinology strategy was applied S protein variant D614G identify highly ranked proteins. In this study, multi-epitope synthetic gene designed using computational strategies variant....