Amongst serious biotic factors deteriorating crop yield, the most destructive pathogen of rice is

Burkholderia glumae and B. gladioli are seed-borne rice pathogens that cause bacterial panicle blight (BPB) disease, resulting in huge yield losses worldwide. However, the excessive use of chemical pesticides agriculture has led to an increase environmental toxicity. Microbe-mediated nanoparticles (NPs) have recently gained significant attention owing their promising application plant disease control. In current study, we biologically synthesize zinc oxide (ZnONPs) from a native Bacillus...

Abstract Nanoparticles (NPs) have emerged as a revolutionary strategy in the field of agriculture, offering innovative solutions for enhancing plant health, disease management, and sustainable crop production. This review summarizes multifaceted roles NPs, synthesized chemically biologically, encompassing NP modulation immunity against pathogens, mechanisms uptake, potential applications control. The integration NPs delivery vehicles bioactive molecules, enabling targeted nutrients,...

Rice constitutes a foundational cereal and plays vital role in the culinary sector. However, detriments of abiotic stress on rice quality productivity are noteworthy. Carotenoid cleavage oxygenases ( CCO ) hold importance as they enable particular breakdown carotenoids significantly contribute towards growth response to rice. Due insufficient information regarding CCOs their potential stress, utilization stress-resistant genetic breeding remains limited. The current research identified 16...

Haemophilus parainfluenzae is a Gram-negative opportunist pathogen within the mucus of nose and mouth without significant symptoms has an ability to cause various infections ranging from ear, eye, sinus pneumonia. A concerning development increasing resistance H. beta-lactam antibiotics, with potential dental or abscesses. The principal objective this investigation utilize bioinformatics immuno-informatic methodologies in candidate multi-epitope Vaccine. focuses on identifying epitopes for...

By using a transcriptomic approach, we have elucidated the effect of Ni(2+) on global gene expression S. pneumoniae D39 by identifying several differentially expressed genes/operons in presence high extracellular concentration Ni(2+). The genes belonging to AdcR regulon (adcRCBA, adcAII-phtD, phtA, phtB, and phtE) PsaR (pcpA, prtA, psaBCA) were highly upregulated We further studied role regulation ICP-MS analysis, electrophoretic mobility shift assays transcriptional lacZ-reporter studies,...

Plant growth promoting rhizobacteria have the potential to mitigate abiotic stressors improve plant productivity. The current study was conducted isolate and characterize halo-tolerant PGPR strains from native plants of Khewra salt mine in Pakistan. Two bacterial (SBN01 SBN02) were purified, examined vitro for their growth-promoting traits, inoculated on wheat evaluate beneficial effects. Both isolates able produce indole-acetic acid tryptophan-supplemented media, both N2 fixers capable...

GpsB links peptidoglycan synthases to other proteins that determine the shape of respiratory pathogen Streptococcus pneumoniae (pneumococcus; Spn) and low-GC Gram-positive bacteria. is also required for phosphorylation by essential StkP(Spn) Ser/Thr protein kinase. Here we report three classes frequently arising chromosomal duplications (≈21-176 genes) containing murZ (MurZ-family homolog MurA) or murA suppress ΔgpsB ΔstkP. These arose from different repeated sequences demonstrate facility...

Abstract We describe the development, characterization, and biological testing of a new type linear molecularly imprinted polymer (LMIP) designed to act as an anti‐infective by blocking quorum sensing (QS) mechanism so abrogating virulence pathogen Streptococcus pneumoniae . The LMIP is prepared (polymerized) in presence template molecule, but unlike traditional molecular imprinting approaches, no cross‐linker used. This results soluble low‐molecular‐weight oligomers that can therapeutic...

Abstract Microbes communicate with each other by using quorum sensing (QS) systems and modulate their collective ‘behavior’ for in-host colonization virulence, biofilm formation, environmental adaptation. The recent increase in genome data availability reveals the presence of several putative QS circuits microbial pathogens, but many these have not been functionally characterized yet, despite possible utility as drug targets. To repertoire bacteria, we studied Rgg144/Shp144 Rgg939/Shp939,...

Background: Non-toxic, non-volatile organic acid, gluconic acid (pentahydroxycaproic acid) is found in abundance honey and plants wines. Gluconic its salts are used widely food, feed, pharmaceuticals, textiles, many other industries.Methods: In the current investigation, Aspergillus niger was utilized for production submerged fermentation. Furthermore, hyperproduction, fungus exposed to MNNG (100 µg/ml) 15 minutes. strain (parent mutant) tested identified capability of producing acid. We...

Mediterraneibacter gnavus , also known as Ruminococcus is a Gram-positive anaerobic bacterium that resides in the human gut microbiota. Notably, this plays dual roles health and disease. On one side it supports nutrient metabolism essential for bodily functions on other contributes to development of Inflammatory Bowel Disease (IBD) gastrointestinal disorders. R. strain RJX1120 an encapsulated has been linked develop IBD. Despite advances made its role homeostasis, limited information...

The bacterial species employ various types of molecular communication systems recognized as quorum sensing for the synchronization differential gene expression to regulate virulence traits and biofilm formation. A variety inhibitors; molecules that interfere with among bacteria have been examined which can block action autoinducers. Moreover, studies scrutinized enzymes their quenching activity resulting in degradation signaling or blocking expression. So far, found these approaches are not...

RodZ of rod-shaped bacteria functions to link MreB filaments the Rod peptidoglycan (PG) synthase complex that moves circumferentially perpendicular long cell axis, creating hoop-like sidewall PG. Ovoid-shaped bacteria, such as Streptococcus pneumoniae (pneumococcus; Spn) lack MreB, use a different modality for peripheral PG elongation emanates from midcell dividing cells. Yet, S. encodes homolog similar in bacteria. We show here helix-turn-helix and transmembrane domains RodZ(Spn) are...

The purpose of this research was to investigate the possible antibacterial, antifungal, antioxidant, and anticancer effects nickel (Ni2+)-doped hydroxyapatite (HAp) nanoparticles (NPs) synthesized using sol–gel approach. X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Raman (Raman), field-emission scanning electron microscopy (FESEM), elemental analysis were used characterize Ni2+-doped HApNPs. investigation showed that nanoscale structure HApNPs hexagonal,...

Pyrimidine nucleotides play an important role in the biosynthesis of activated nucleotide sugars (NDP-sugars). NDP-sugars are precursors structural polysaccharides bacteria, including capsule, which is a major virulence factor human pathogen S. pneumoniae. In this work, we identified spontaneous non-reversible mutant strain D39 that displayed non-producing capsule phenotype. Whole-genome sequencing analysis revealed several non-synonymous single base modifications, genes de novo synthesis...

Solanum tuberosum (STP) and Pisum sativum peels (PSP) were used for removal of toxic Congo Red dye using indigenous sources. Potato (Solanum tuberosum) pea (Pisum sativum) are commonly abundantly cultivated plants in Asian countries their easily available. The optimized conditions were: adsorbent dose; 0.8 0.6 g, contact time; 50 35 min, pH; 3 2, agitation speed; 200 125 rpm temperature; 30 oC both STP PSP respectively. Isothermal studies indicated that Langmuir model followed by equilibrium...

In this study, we have explored the transcriptomic response of Streptococcus pneumoniae D39 to N-acetylglucosamine (NAG). Transcriptome comparison S. wild-type grown in chemically defined medium (CDM) presence 0.5% NAG that glucose revealed elevated expression many genes/operons, including nagA, nagB, manLMN, and nanP. We further confirmed NAG-dependent nanP by β-galactosidase assays. nagB glmS are putatively regulated a transcriptional regulator NagR. predicted operator site NagR (dre site)...

Stripe rust is a continuous threat to wheat crop all over the world. It causes considerable yield losses in every year. Continuous deployment of adult plant resistance (APR) genes newly developing cultivars most judicious strategy combat this disease. Herein, we dissected genetics underpinning stripe Pakistani germplasm. An association panel 94 spring genotypes was phenotyped for two years score infestation on each accession and scanned with 203 polymorphic SSRs. Based D´ measure, linkage...

Waterbirds may be a good indicator of harmful metal levels in aquatic environments. Waterbirds’ organs and tissues were tested for the presence pollutants, such as metals. However, very few reports describe use bird feathers their prey analysis. In present research, seven metals measured tissue, kidney, liver, Indian pond heron, black-crowned night species, including crabs, prawns, molluscs, fishes from freshwater lake. Metals examined using an ECIL-4141-double beam atomic absorption...

Streptococcus pneumoniae is able to cause deadly diseases by infecting different tissues, each with distinct environmental and nutritional compositions. We hypothesize that the adaptive capabilities of microbe an important facet pneumococcal survival in fluctuating host environments. Quorum-sensing (QS) mechanisms are pivotal for microbial adaptation. previously demonstrated TprA/PhrA QS system required utilization galactose mannose, neuraminidase activity, virulence. also showed can be...