A5078506070- Biochemical and Molecular Research
- Helicobacter pylori-related gastroenterology studies
- Clostridium difficile and Clostridium perfringens research
- Microbial Metabolites in Food Biotechnology
- Carbohydrate Chemistry and Synthesis
- Enzyme Structure and Function
- Galectins and Cancer Biology
- Veterinary medicine and infectious diseases
- Gastric Cancer Management and Outcomes
- Enzyme Production and Characterization
University of Warsaw
2020-2024
The requirement for fast and dependable protein purification methods is constant, either functional studies of natural proteins or the production biotechnological products. original procedure has to be formulated each individual protein, this demanding task was significantly simplified by introduction affinity tags. Helicobacter pylori adenylosuccinate synthetase (AdSS) present in solution a dynamic equilibrium monomers biologically active homodimers. addition His6-tag on C-terminus...
Purine nucleotide synthesis is realised only through the salvage pathway in pathogenic bacterium Helicobacter pylori. Therefore, enzymes of this pathway, among them also adenylosuccinate synthetase (AdSS), present potential new drug targets. This paper describes characterization His6-tagged AdSS from H. Thorough analysis 3D-structures fully ligated (in a complex with guanosine diphosphate, 6-phosphoryl-inosine monophosphate, hadacidin and Mg2+) inosine monophosphate (IMP) only, enabled...
Helicobacter pylori represents a global health threat with around 50% of the world population infected. Due to increasing number antibiotic-resistant strains, new strategies for eradication H. are needed. In this study, we suggest purine nucleoside phosphorylase (PNP) as possible drug target, by characterising its interactions 2- and/or 6-substituted purines well effect these compounds on bacterial growth. Inhibition constants in micromolar range, lowest being that...
The current therapies against gastric pathogen Helicobacter pylori are ineffective in over 20% of patients. Enzymes belonging to the purine salvage pathway considered as novel drug targets this pathogen. Therefore, main aim study was determine antibacterial activity pyridoxal 5'-phosphate (PLP), an active form vitamin B6, reference and clinical strains H. pylori. Using a broad set microbiological, physicochemical (UV absorption, LC-MS, X-ray analysis) silico experiments, we were able prove...
Due to the growing number of Helicobacter pylori strains resistant currently available antibiotics, there is an urgent need design new drugs utilizing different molecular mechanisms than those that have been used up now. Enzymes purine salvage pathway are possible targets such antibiotics because H. not able synthetize nucleotides de novo. The bacterium's recovery purines and from environment only source these essential DNA RNA building blocks. We identified formycins hadacidin as potent...