A5056838759- Respiratory Support and Mechanisms
- RNA Interference and Gene Delivery
- RNA regulation and disease
- Aortic Disease and Treatment Approaches
- Cardiac Valve Diseases and Treatments
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Neonatal Respiratory Health Research
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- interferon and immune responses
- Airway Management and Intubation Techniques
- Infective Endocarditis Diagnosis and Management
- Estrogen and related hormone effects
- Cardiac Arrest and Resuscitation
- Aortic aneurysm repair treatments
- Chronic Obstructive Pulmonary Disease (COPD) Research
- COVID-19 epidemiological studies
- Viral Infections and Immunology Research
- RNA modifications and cancer
- Mycobacterium research and diagnosis
- Gene expression and cancer classification
- Autoimmune and Inflammatory Disorders Research
- Rheumatoid Arthritis Research and Therapies
- Multiple Sclerosis Research Studies
Vanderbilt University
2016-2025
Nashville Oncology Associates
2022
University of Pittsburgh
2005-2014
VA Pittsburgh Healthcare System
2007-2014
California State University, Chico
2014
Centennial Medical Center
2012-2013
Dr. Margarete Fischer-Bosch-Institute of Clinical Pharmacology
2011
Regions Hospital
2001-2005
University of Minnesota
1989-2005
National Institutes of Health
2005
Long noncoding RNAs (lncRNAs) regulate an array of biological processes in cells and organ systems. Less is known about their expression function lymphocyte lineages. Here we have identified >2000 lncRNAs expressed human T-cell cultures those that display a TH lineage-specific pattern are intragenic or adjacent to genes encoding proteins with immunologic functions. One lncRNA cluster selectively by the effector TH2 lineage consists four alternatively spliced transcripts cytokines, IL-4, IL-5...
In recent years, four square-wave modes of pressure-preset mechanical ventilation (PPV)--pressure control, pressure support, inverse ratio, and airway release ventilation--have been introduced to clinical practice. Conceptually, they share important features. Yet, because there remains widespread uncertainty regarding their ventilatory characteristics, efficacy, appropriate use, the potential range application is only now being investigated. To construct a unifying mathematical model PPV, we...
This study assessed the safety and efficacy of an internal geometric annuloplasty ring in a regulatory trial aortic valve reconstruction (ClinicalTrials.gov Identifier: NCT01400841). Sixty-five patients with predominant moderate-to-severe trileaflet insufficiency (AI) underwent repair average age 63 ± 13 years (mean SD). All had initial implantation to correct annular dilatation facilitate leaflet reconstruction. Leaflet plication was performed for prolapse 80% patients, more complex...
Abstract Clinicians currently monitor pressure and volume at the airway opening, assuming that these observations relate closely to stresses strains micro level. Indeed, this assumption forms basis of current approaches lung protective ventilation. Nonetheless, although applied under static conditions may be same everywhere in healthy lungs, within a mechanically non-uniform ARDS are not. Estimating actual tissue occur environment must account for factors beyond measurements from ventilator...
Low-dose methotrexate (MTX) is an effective therapy for rheumatoid arthritis (RA), yet its mechanism of action incompletely understood. The aim this study was to explore the induction apoptosis by MTX.Flow cytometry performed assess changes in levels intracellular proteins, reactive oxygen species (ROS), and apoptosis. Quantitative polymerase chain reaction transcript select target genes response MTX.MTX did not directly induce but rather "primed" cells markedly increased sensitivity via...
Determining how alveoli are formed and maintained is critical to understanding lung organogenesis regeneration after injury. To study the cellular dynamics of this stage development, we have used scanned oblique-plane illumination microscopy living slices observe alveologenesis in real time at high resolution over several days. Contrary prevailing notion that occurs by airspace subdivision via ingrowing septa, find form ballooning epithelial outgrowth supported contracting mesenchymal ring...
Endogenous Alu RNAs form double-stranded recognized by RNA sensors and activate IRF NF-kB transcriptional paths innate immunity. Deamination of adenosines to inosines the ADAR family enzymes, a process termed A-to-I editing, disrupts structure prevents immune activation. Innate activation is observed in Alzheimer's disease, most common dementia. We have previously reported loss editing hippocampus vasculature, but no change cortex or associated with disease. Here, we investigated status...
Objective: To develop and disseminate a spatially explicit model of contact transmission pathogens in the intensive care unit. Design: A simulating spread pathogen transmitted by direct (such as methicillin-resistant Staphylococcus aureus or vancomycin-resistant Enterococcus) was constructed. The modulation dissemination attending changes clinically relevant pathogen- institution-specific factors then systematically examined. Setting Patients: configured hypothetical 24-bed can be...
Abstract We employed whole-genome RNA-sequencing to profile mRNAs and both annotated novel long noncoding RNAs (lncRNAs) in human naive, central memory, effector memory CD4+ T cells. Loci transcribing lineage-specific lncRNA are adjacent protein-coding genes the genome. Lineage-specific loci transcribed from typical- supertranscriptional enhancers not multiexonic, thus more similar enhancer RNAs. Novel enhancer-associated lncRNAs IFNG locus bind transcription factor NF-κB enhance binding of...
Abstract Oxidative metabolites of estrogens have been implicated in the development breast cancer, yet relatively little is known about metabolism normal breast. We developed a mathematical model mammary estrogen based on conversion 17β-estradiol (E2) by enzymes cytochrome P450 (CYP) 1A1 and CYP1B1, catechol-O-methyltransferase (COMT), glutathione S-transferase P1 into eight [i.e., two catechol estrogens, 2-hydroxyestradiol (2-OHE2) 4-hydroxyestradiol (4-OHE2); three methoxyestrogens,...
Surgery for aortic root aneurysm without valve stenosis is increasingly being transformed from the Bentall procedure to valve-sparing remodelling or reimplantation. In this report, a new repair option explored, with full functional 'restoration' of complex using geometric annuloplasty ring, leaflet repair, and sinus/ascending replacement Valsalva graft. The ring restores elliptical annular shape size in patients tri-leaflet insufficiency (AI). mounts three commissures on 10° outwardly...
Severe COVID-19 disease is associated with elevated inflammatory responses. One form of Aicardi-Goutières syndrome caused by inactivating mutations in ADAR results reduced adenosine-to-inosine (A-to-I) editing endogenous dsRNAs, induction IFNs, IFN-stimulated genes, other mediators, morbidity, and mortality. Alu elements, ∼10% the human genome, are most common A-to-I-editing sites. Using leukocyte whole-genome RNA-sequencing data, we found A-to-I dsRNAs patients severe disease. Dendritic...
Head-elevated body positioning, a default clinical practice, predictably increases end-expiratory transpulmonary pressure and aerated lung volume. In acute respiratory distress syndrome (ARDS), however, the net effect of such vertical inclination on tidal mechanics depends upon whether recruitment or overdistension predominates. We hypothesized that in moderate to severe ARDS, bed toward unloads chest wall but adversely affects overall system compliance (C rs ).Prospective physiologic...
Estrogen is a well-known risk factor for breast cancer. Current models of cancer prediction are based on cumulative estrogen exposure but do not directly reflect mammary metabolism or address genetic variability between women in to carcinogenic metabolites. We proposing mathematical model that forecasts woman three factors: (1) estimated exposure, (2) kinetic analysis the oxidative pathway breast, and (3) enzyme genotypes responsible inherited differences production The incorporates main...