A5036745276- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Heat shock proteins research
- Plant Stress Responses and Tolerance
- Ubiquitin and proteasome pathways
- Plant biochemistry and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Genetics, Aging, and Longevity in Model Organisms
- Vaccine Coverage and Hesitancy
- Hepatitis B Virus Studies
- Plant Molecular Biology Research
- Seed Germination and Physiology
- Immune Response and Inflammation
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Biofuel production and bioconversion
- Innovative Teaching Methods
- Fungal and yeast genetics research
- Liver Disease Diagnosis and Treatment
- Insect and Arachnid Ecology and Behavior
- Enzyme Structure and Function
- Mitochondrial Function and Pathology
- Innovative Teaching and Learning Methods
Indian Institute of Science Education and Research Mohali
2014-2024
National Public Health Laboratory
2015-2020
Ministry of Health and Population
2020
Department of Health Services
2020
Goethe University Frankfurt
2001-2018
Max Planck Institute of Biochemistry
2004-2014
We generated transgenic tomato plants with altered expression of heat stress transcription factor HsfA1. Plants 10-fold overexpression HsfA1 (OE plants) were characterized by a single transgene cassette, whereas harboring tandem inverted repeat the cassette showed cosuppression (CS posttranscriptional silencing gene connected formation small interfering RNAs. Under normal growth conditions, major developmental parameters similar for wild-type (WT), OE, and CS plants. However, fruits...
Male reproductive tissues are more sensitive to heat stress (HS) compared vegetative tissues, but the basis of this phenomenon is poorly understood. Heat transcription factors (Hsfs) regulate transcriptional changes required for protection from HS. In tomato (Solanum lycopersicum), HsfA2 acts as coactivator HsfA1a and one major Hsfs accumulating in response elevated temperatures. The contribution (HSR) thermotolerance was investigated different transgenic plants with suppressed levels...
Abstract Plants code for a multitude of heat stress transcription factors (Hsfs). Three them act as central regulators (HS) response in tomato ( Solanum lycopersicum ). HsfA1a regulates the initial response, and HsfA2 controls acquired thermotolerance. HsfB1 is transcriptional repressor but can also co‐activator HsfA1a. Currently, mode action relevance dual function remain elusive. We examined this overexpression or suppression transgenic lines. Proteome analysis revealed that stimulates...
The heat stress response is universal to all organisms. Upon elevated temperatures, transcription factors (Hsfs) are activated up-regulate the expression of molecular chaperones protect cells against damages. In higher plants, phenomenon unusually complex both at level Hsfs and proteins (Hsps). Over-expression Hsps use RNA interference for gene knock-down in a transient system tomato protoplasts allowed us dissect vivo chaperone functions essential components thermotolerance, such as...
Different from canonical ubiquitin-like proteins, Hub1 does not form covalent conjugates with substrates but binds proteins non-covalently. In Saccharomyces cerevisiae, associates spliceosomes and mediates alternative splicing of SRC1, without affecting pre-mRNA generally. Human is highly similar to its yeast homolog, cellular function remains largely unexplored. Here, we show that human the spliceosomal protein Snu66 as in yeast; however, unlike S. cerevisiae essential for viability....
Intron diversity facilitates regulated gene expression and alternative splicing. Spliceosomes excise introns after recognizing their splicing signals: the 5'-splice site (5'ss), branchpoint (BP) 3'-splice (3'ss). The latter two signals are recognized by U2 small nuclear ribonucleoprotein (snRNP) its accessory factors (U2AFs), but longer spacings between them result in weaker Here, we show that excision of with a BP-distant 3'ss (e.g. rap1 intron 2) requires ubiquitin-fold-activated regulator...
Abstract Undergraduate laboratory courses, owing to their larger sizes and shorter time slots, are often conducted in highly structured modes. However, this approach is known interfere with students' engagement the experiments. To enhance engagement, we propose an alternative mode of running courses by creating some “disorder” a previously adopted structure. After performing experiment right way, students were asked repeat but variation at certain steps leading being done “wrong” way....
The conserved ubiquitin-like protein Hub1/UBL5 functions in RNA splicing, DNA repair and mitochondrial unfolding responses. It binds proteins specific to these pathways modifies their functional properties. However, the identities of other Hub1 substrates remain unknown. We have found unreported interactors Saccharomyces cerevisiae from a yeast two-hybrid (Y2H) screen. Proteins containing SIMs (small modifier SUMO-interaction motifs) ferulic acid decarboxylase Fdc1 are identified as...
Heat shock protein participates in several broad ranges of folding processes and control activity regulatory proteins. Nearly all cells react a similar manner against the external environmental abrupt changes like heat, chemicals such as analogues amino acid, ethanol, arsenite, heavy metals, mitochondrial functional inhibitors, cold, UV light. These causes severe, rapid at level gene expression. The major significant HSP to stabilize newly synthesized by securing its right folding, which...
Abstract Cwc23 is an essential J-domain protein that collaborates with the disassembly factor Ntr1 to facilitate spliceosomal disassembly. Although orthologs have been identified in extracts of many eukaryotes, their functionality intron-rich eukaryotes remains largely unexplored. Here, we investigate Cwf23, ortholog Schizosaccharomyces pombe . Our study reveals while interaction between Cwf23 and Sp conserved, it not S. Additionally, RNA recognition motif (RRM) crucial for its function, as...
Regulated precursor messenger RNA (pre‐mRNA) splicing modulates gene expression and promotes alternative splicing. The process is regulated by modifications of spliceosomal proteins small nuclear RNAs (snRNAs). Here, we show that the protein phosphatase Psr1, known for its plasma membrane localisation function in general stress response Saccharomyces cerevisiae , also plays a regulatory role pre‐mRNA Independently presence at membrane, Psr1 binds dephosphorylates core factor Snu66. enzyme...
The ubiquitin-like protein Hub1/UBL-5 associates with proteins non-covalently. Hub1 promotes alternative splicing and of precursor mRNAs weak introns in yeast mammalian cells; however, its function has remained elusive multicellular organisms. Here, we demonstrate the free-living nematode Caenorhabditis elegans. binds to HIND-containing factors Snu66/SART-1 PRP-38 other spliceosomal proteins. C. elegans hub1/ubl-5 mutants die at Larval 3 stage show defects for selected targets, similar...
Hepatitis B virus (HBV) infects the liver, causing cirrhosis and cancer. In developed countries, five international guidelines have been used to make a decision for management of patients with chronic HBV infection. this review, since were established by clinical epidemiological data we aimed evaluate whether (1) patient profiles developing countries are similar (2) which guideline can be applicable resource-limited countries. First, as an example most recent infections among evaluated...
TLR8 assists in antiviral approach by producing Type 1 INF via MyD88 dependent IRF7 pathway. However, over expression of INFα/β molecule poses threat developing tolerance chronic infection cases and enhancing inflammatory response. Here we report a bi-specific siRNA based complex which differentially activates silences the MYD88 respectively negatively regulated fashion. Outer membrane vesicle from Escherichia coli used for delivery was observed more efficient when attached with invasive...
Knocking out a chromatin factor often does not alter the transcription of its binding targets. What explains observed disconnect between and effect? We hypothesize that this discrepancy could be associated with role factors in maintaining genetic epigenetic integrity at promoters, necessarily transcription. Through re‐analysis published datasets, we present several lines evidence support our hypothesis deflate popular assumptions. also tested through mutation accumulation assays on yeast...