A5006645417- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Ion Channels and Receptors
- Pain Mechanisms and Treatments
- Neurobiology and Insect Physiology Research
- Neuroendocrine regulation and behavior
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Sleep and Wakefulness Research
- Nitric Oxide and Endothelin Effects
- Neuroscience of respiration and sleep
- Hormonal Regulation and Hypertension
- Anesthesia and Neurotoxicity Research
- Cancer, Stress, Anesthesia, and Immune Response
- Renal function and acid-base balance
- Magnesium in Health and Disease
- Advanced Chemical Sensor Technologies
- Electrolyte and hormonal disorders
- Sphingolipid Metabolism and Signaling
- Healthcare and Venom Research
- Circadian rhythm and melatonin
- Venomous Animal Envenomation and Studies
- Migraine and Headache Studies
- Genetics and Neurodevelopmental Disorders
- Calcium signaling and nucleotide metabolism
Centre National de la Recherche Scientifique
2014-2023
Université Côte d'Azur
2014-2023
Inserm
1999-2023
Laboratoire d'Excellence Canaux Ioniques d'Intérêt Thérapeutique
2013-2023
Clermont Université
2023
Université Clermont Auvergne
2023
Institut de Pharmacologie Moléculaire et Cellulaire
2012-2022
Institut de Biologie Valrose
2021
Observatoire de la Côte d’Azur
2018
Montserrat Volcano Observatory
2009
MDEG1 is a cation channel expressed in brain that belongs to the degenerin/epithelial Na+channel superfamily. It activated by same mutations which cause neurodegeneration Caenorhabditis elegans if present degenerins DEG-1, MEC-4, and MEC-10. shares 67% sequence identity with recently cloned proton-gated ASIC (acid sensing ion channel), new member of family sensory neurons. We have now identified as properties different from those ASIC. requires more acidic pH values for activation has slower...
A complementary DNA encoding an amiloride‐sensitive Na + channel has been cloned and characterized from rat colon. The protein encoded by the cDNA a sequence of 699 amino acids (79 kDa) containing several putative membrane spanning domains potential phosphorylation sites. It forms that electrophysiological pharmacological properties characteristic epithelial channel. Homologies (including in transmembrane domains) have found between part Mec4 gene product Caenorhabditis elegans , associated...
Water balance in the lung is controlled via active Na+ and Cl- transport. Electrophysiological measurements on epithelial cells demonstrated presence of a channel that inhibited by amiloride (K0.5 = 90 nM) some its derivatives such as phenamil 19 benzamil 14 but not ethylisopropylamiloride. An amiloride-sensitive 4 pS was recorded from outside-out patches excised apical membrane. This highly selective for (PNa+/PK+ > or to 10). Isolation human cDNA led primary structure channel. The...
A key protein component of the amiloride-sensitive sodium channel has been cloned from rat colon and human lung. It may represent first member a new family ionic channels expressed nematode to human. The biochemical properties protein, 699 amino acids long polypeptide, have analyzed. Four polyclonal antibodies raised against distinct parts immunoprecipitated glycosylated 96 kDa after cRNA expression in oocytes as well vitro translation. When alone into oocytes, was not stable; most it...
Acid-sensing ion channels (ASICs) are cationic activated by extracellular protons. They expressed in sensory neurons, where they thought to be involved pain perception associated with tissue acidosis. also brain. A number of brain regions, like the hippocampus, contain large amounts chelatable vesicular Zn(2+). This paper shows that Zn(2+) potentiates acid activation homomeric and heteromeric ASIC2a-containing (i.e. ASIC2a, ASIC1a+2a, ASIC2a+3), but not ASIC1a ASIC3. The EC(50) for...
Iatrogenic pain consecutive to a large number of surgical procedures has become growing health concern. The etiology and pathophysiology postoperative are still poorly understood, but hydrogen ions appear be important in this process. We have investigated the role peripheral acid-sensing ion channels (ASICs), which form depolarizing activated by extracellular protons, rat model (i.e., hindpaw skin/muscle incision). report high levels ASIC-type currents (∼77%) sensory neurons innervating...
Long term regulation of the amiloride-sensitive Na+ channel activity by steroid hormones occurs via de novo protein synthesis. The messenger level RCNaCh1, previously shown expression cloning to be a component this channel, was measured in colons from rats fed with low sodium diet. After 1 week diet, increased an all-or-none fashion, whereas RCNaCh1 remained constant. A cDNA coding for another subunit obtained polymerase chain reaction. 650-amino acid protein, entitled RCNaCh2, is 58%...
Acid-sensing ion channels (ASICs) are broadly expressed in the CNS, including spinal cord. However, very little is known about properties of ASICs cord neurons compared with brain. We show here that ASIC1a and ASIC2a most abundant mouse adult coexpressed by throughout all laminas. ASIC currents cultured embryonic day 14 dorsal mainly flow through homomeric (34% neurons) heteromeric plus 2a at a ratio 2:1 (83% neurons). ASIC2b only has minor contribution to these currents. The two channel...
In rodent sensory neurons, acid-sensing ion channel 3 (ASIC3) has recently emerged as a particularly important sensor of nonadaptive pain associated with tissue acidosis. However, little is known about the human ASIC3 channel, which includes three splice variants differing in their C-terminal domain (hASIC3a, hASIC3b, and hASIC3c). hASIC3a transcripts represent main mRNAs expressed both peripheral central neuronal tissues (dorsal root ganglia [DRG], spinal cord, brain), where small...
Mambalgins are 57-amino acid peptides isolated from snake venom that evoke naloxone-resistant analgesia after local (intraplantar) and central (intrathecal) injections through inhibition of particular subtypes acid-sensing ion channels (ASICs). We now show mambalgins also have an opioid-independent effect on both thermal mechanical inflammatory pain systemic intravenous (i.v.) administration effective against neuropathic pain. By combining the use knockdown knockout animals, we critical...
The Helix aspersaPhe-Met-Arg-Phe-amide (FMRFamide)-gated sodium channel is formed by homomultimerization of several FMRFamide-activated Na+channel (FaNaCh) proteins. FaNaCh homologous to the subunits that compose amiloride-sensitive epithelial channel, Caenorhabditis elegans degenerins, and acid-sensing ionic channels. properties were analyzed in stably transfected human embryonic kidney cells (HEK-293). was functional with an EC50 for FMRFamide 1 μm IC50 (25 °C) amiloride 6.5 as assessed...
ASIC3, an acid-sensing ion channel subunit expressed essentially in sensory neurons, has been proposed to be involved pain. We show here for the first time that native ASIC3-like currents were increased cultured dorsal root ganglion (DRG) neurons following protein kinase C (PKC) stimulation. This increase was induced by phorbol ester PDBu and pain mediators, such as serotonin, which are known activate PKC pathway through their binding G protein-coupled receptors. demonstrate this regulation...