A5079524825- Silk-based biomaterials and applications
- Invertebrate Immune Response Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Silkworms and Sericulture Research
- Advanced Chemical Sensor Technologies
- Tryptophan and brain disorders
- Gas Sensing Nanomaterials and Sensors
- Antimicrobial Peptides and Activities
- Insect and Arachnid Ecology and Behavior
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- CRISPR and Genetic Engineering
- Insect symbiosis and bacterial influences
- Respiratory and Cough-Related Research
- Plant Virus Research Studies
- RNA Research and Splicing
- Analytical Chemistry and Sensors
- Neurobiology and Insect Physiology Research
- Biochemical and Structural Characterization
- Cancer, Stress, Anesthesia, and Immune Response
- Insect Resistance and Genetics
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
Zhejiang University
2016-2024
Zhejiang Lab
2023
Spider dragline silk is a remarkable material that shows excellent mechanical properties, diverse applications, biocompatibility and biodegradability. Transgenic silkworm technology was used to obtain four types of chimeric silkworm/spider (termed composite) fibres, including different lengths recombinant Major ampullate Spidroin1 (re-MaSp1) or Spidroin2 (re-MaSp2) from the black widow spider, Latrodectus hesperus. The results showed overall properties composite fibres improved as re-MaSp1...
Early screening of individuals with high-risk lung nodules can significantly improve the prognosis cancer patients, and accurate identification nodule subtypes provide guidance for medical treatment. Exhaled breath (EB) analysis via eNoses offers a quick noninvasive approach, but current eNose technology lacks quality control solid validation in large population studies. Herein, an platform integrated metal ion-decorated graphene sensor array sampling accessory was established. EB samples...
Abstract Spider silk is one of the best natural fibers with excellent mechanical properties; however, due to visual awareness, biting behavior and territory consciousness spiders, we cannot obtain spider by large-scale breeding. Silkworms have a spinning system similar that use transgenic technology in Bombyx mori , which an ideal reactor for producing silk, routine. In this study, piggyBac transposon technique was used achieve specific expression two putative genes posterior glands...
Silkworm, as a model organism, has very high economic value due to its silk secretion ability. Although large number of studies have attempted elucidate the mechanism secretion, it remains unclear. In this study, fibroin light chain (Fib-L) gene silkworm was subjected CRISPR/Cas9 editing, which yielded premature termination translation at 135 aa. Compared with those wild type, posterior glands (PSGs) homozygous mutants on third day fifth instar showed obvious degeneration. Comparative...
This paper explores the structures of exogenous protein molecules that can effectively improve mechanical properties silkworm silk. Several transgenic vectors fused with fibroin light chain and type 3 repeats in different multiples ampullate dragline silk 1 (MaSp1) from black widow spider lengths polyalanine motifs were constructed for this study. Transgenic silkworms successfully obtained by piggyBac-mediated microinjection. Molecular detection showed foreign proteins secreted contained...
Extensive research has been conducted on utilizing transgenic silkworms and their natural spinning apparatus to produce high-performance spider silk fibers. However, using non-spider biological proteins optimize the molecular structure of protein improve mechanical performance fibers is still relatively scarce. Dumpy, a massive extracellular matrix polypeptide, essential for preserving shape structural integrity insect cuticle due its remarkable tension elasticity. Here, we constructed two...
Silkworm was the first domesticated insect and has important economic value. It also become an ideal model organism with applications in genetic expression studies. In recent years, use of transgenic strategies made silkworm silk gland attractive bioreactor for production recombinant proteins, particular,
The wild silkworm Bombyx mandarina was domesticated to produce silk in China approximately 5000 years ago. Silk production is greatly improved the B. mori, but molecular basis of functional evolution gland remains elusive. We performed shotgun proteomics with label-free quantification analysis and identified 1012 822 proteins from posterior glands (PSGs) silkworms on third fifth days instar, respectively, 128 these differentially expressed. Bioinformatics revealed that, development PSG,...
Transgenic silkworm expression systems have been applied for producing various recombinant proteins. Knocking out or downregulating an endogenous silk protein is considered a viable strategy improving the ability of transgenic to produce exogenous Here, we report human epidermal growth factor (hEGF) in P25 gene knockout silkworm. The hEGF regulated by promoter was integrated into silkworm’s genome. Five positive lineages were generated with different insertion sites on chromosomes and...
Abstract Exhaled breath provides information of individual's holistic physiological condition, making non‐invasive exhaled testing a convenient and effective choice for personalized health management. This work proposes mobile e‐nose prototype equipped with miniaturized gas delivering unit, an advanced sensor array, customized analog to digital conversion circuit board, online data transmission, machine learning algorithms that can effectively detect analyze volatile compounds in breath,...
Naked pupa sericin and are two mutant strains of Bombyx mori with extremely low or no fibroin production compared to the Qiufeng Baiyu strains, both which exhibit very high silk production. However, molecular mechanisms by long non-coding RNAs regulate synthesis need further study. In this study, we performed high-throughput RNA-seq investigate lncRNA mRNA expression profiles in posterior gland Qiufeng, Baiyu, Nd-s D , Nd silkworms at third day 5th instar. Our efforts yielded 26,767 novel...
The domestic silkworm is a type of lepidopteran insect that feeds on mulberry leaves and has high economic value because its ability to spin cocoons. Sericin 1 an important component cocoons, accounting for approximately 25% the material. In this study, CRISPR/Cas9-mediated gene editing was successfully used destroy sericin gene, homozygous mutants were obtained after continuous screening. Homozygous mutation resulted in premature termination translation protein at 323 amino acids....
Resilin is a natural protein with high extensibility and resilience that plays key role in the biological processes of insects, such as flight, bouncing, vocalization. This study used piggy Bac-mediated transgenic technology to stably insert Drosophila melanogaster resilin gene into silkworm genome investigate whether exogenous structures improve mechanical properties silk. Molecular detection showed recombinant was expressed secreted Secondary structure property analysis β-sheet content...
The silkworm genome has been deeply sequenced and assembled, but accurate annotation, which is important for modern biological research, remains far from complete. To improve we carried out a proteogenomics analysis using 9.8 million mass spectra collected different tissues developmental stages of the silkworm. results confirmed translational products 4307 existing gene models identified 1701 novel search-specific peptides (GSSPs). Using these GSSPs, 74 gene-coding sequences were identified,...
Human epidermal growth factor (hEGF) is a key involved in wound healing owing to its powerful ability stimulate cell proliferation. In this study, we used piggyBac transposon technology produce transgenic silkworms expressing the hEGF protein fused truncated heavy chain (FibH-hEGF). The FibH–hEGF fusion was successfully expressed and secreted into silkworm cocoons. Compared wild-type silk, silk had similar morphology about silks fiber surface cocoon nets, while secondary structure between...
To probe the general phenomena of gene mutations, Bombyx mori, lepidopterous model organism, was chosen as experimental model. easily detect phenotypic variations, piggyBac system utilized to introduce two marker genes into silkworm, and 23.4% transposition efficiency aided in breeding a new strain for entire experiment. Then, clustered regularly interspaced short palindromic repeats/an associated protein (Cas9) utilized. The results showed that Cas9 can induce efficient mutations base...
Abstract Indoleamine-2,3-dioxygenase (IDO1) is well-recognized as an important target for immunotherapeutic intervention. Growing interests on this have been demonstrated by the recent expansion of enthusiasms to inhibit Trp-to-Kyn pathway a means control immunosuppression cancer treatment. Clinical validation IDO1 inhibitors treating various tumors including glioblastoma, melanoma, non-small cell lung, pancreatic, and/or breast cancer, well metastatic diseases are currently pursued...
Abstract Indoleamine-2,3-dioxygenase (IDO1) is an immune regulatory enzyme that oxidizes tryptophan to kynurenine. IDO1 overexpressed in numerous tumor cells block T-cell activation, induce apoptosis and increase T cells, which create environment tumor-specific cytotoxic lymphocytes are no longer able attack a patient's cancer cells. inhibition reverses the suppression at site allows generation of effective anticancer response. Preclinical clinical studies have shown inhibitors response...