A5102004715- Virus-based gene therapy research
- RNA modifications and cancer
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Natural product bioactivities and synthesis
- Temporomandibular Joint Disorders
- Phytochemistry and Biological Activities
- Viral gastroenteritis research and epidemiology
- Reproductive System and Pregnancy
- Neuroscience of respiration and sleep
- Adipose Tissue and Metabolism
- CAR-T cell therapy research
- Dental Radiography and Imaging
- Glycosylation and Glycoproteins Research
- Berberine and alkaloids research
- Dental Implant Techniques and Outcomes
- Biological Activity of Diterpenoids and Biflavonoids
- Pregnancy and Medication Impact
- PI3K/AKT/mTOR signaling in cancer
- RNA regulation and disease
- Polyamine Metabolism and Applications
- Biomedical Research and Pathophysiology
- Pancreatic and Hepatic Oncology Research
- Gallbladder and Bile Duct Disorders
- Viral Infectious Diseases and Gene Expression in Insects
University of Kansas Medical Center
2023-2025
Shanghai Jiao Tong University
2005-2024
Shandong First Medical University
2022
Shandong Provincial Hospital
2016-2022
Second Affiliated Hospital of Jilin University
2020
Shandong University
2016-2020
Shandong Provincial QianFoShan Hospital
2016
Creative Commons
2016
X-Fab (Germany)
2016
Christie's
2016
This study is to explore the therapeutic effect and potential mechanisms of exosomal microRNAs (miRNAs) derived from ovaries with primary ovarian insufficiency (POI). The POI mouse model was established by intraperitoneal injection cyclophosphamide (CTX) busulfan. apoptosis granulosa cells (GCs) incubated exosomes extracted tissues control groups analyzed flow cytometry. Then, high-throughput sequencing performed detect difference miRNAs profile in tissue-derived between mice. differential...
Cystic fibrosis (CF) is caused by mutations in the cystic transmembrane conductance regulator (CFTR) gene. While CRISPR-based CFTR editing approaches have shown proof-of-concept for functional rescue primary airway basal cells, induced pluripotent stem and organoid cultures derived from patients with CF, their efficacy remains suboptimal. Here, we developed CuFiCas9(Y66S)eGFP reporter system integrating spCas9 a non-fluorescent Y66S eGFP mutant into CuFi-8 an immortalized human epithelial...
ABSTRACT Recombinant (r)AAV2.5T was selected from the directed evolution of an adeno-associated virus (AAV) capsid library in human airway epithelium (HAE). The gene rAAV2.5T is a chimera N-terminal unique coding sequence AAV2 VP1 (VP1u) and VP2- VP3-coding sequences AAV5 with single amino acid mutation A581T. We conducted two rounds genome-wide CRISPR guide (g)RNA screening for host factors limiting transduction HeLa S3 cells. identified several genes that are critical rAAV2.5T, including...
We investigated and compared 2 clinical strategies to prevent postendoscopic retrograde cholangiopancreatography (ERCP) pancreatitis (PEP). retrospectively reviewed data from patients who underwent ERCP between 2008 2014. Of 623 at high risk for PEP, 145 were treated with prophylactic pancreatic stent placement (PSP) only, 478 rectal indomethacin (RI) PEP prevention. Patients matched by one-to-one propensity score matching (PSM) factors, overall incidence as primary outcome, moderate or...
Abstract Colorectal cancer (CRC) is a leading cause of cancer-related human deaths. The exopolysaccharide (EPS1-1), isolated from Rhizopus nigricans, has been described as exhibiting anti-tumor and pro-apoptotic activity against CRC, although the underlying mechanism poorly understood. Herein, we investigate how EPS1-1 induces apoptosis CRC cells in vitro vivo. Our results show that, vitro, suppressed cell growth facilitated dose- time-dependent manner by activating AMP-activated protein...
Variants in
Background . Long noncoding RNAs (lncRNAs) were previously found to be closely related the pathogenesis of diabetes. Objectives To reveal differentially expressed lncRNAs and messenger (mRNAs) involved in type 2 diabetes mellitus (T2DM) latent autoimmune adults (LADA) predict lncRNA target genes derive their expression profiles for diagnosis T2DM LADA differential diagnosis. Methods Twelve venous blood samples collected from patients, nondiseased subjects obtain total RNAs. After removing...
A large mandibular discontinuity defect is most often due to severe trauma, infection or resection for neoplasm. Reconstruction plates have been popularly applied bridge defects without bone grafting. The esthetics and continuity of the mandible with segmental could be rehabilitated successfully. However, function difficult reconstructed using this type plate. Furthermore, skin mucosa perforation, plate fracture loss screw retention were serious complications resulting in reconstruction...
We conducted a genome-wide CRISPR/Cas9 screen in suspension 293-F cells transduced with rAAV5. The highly selected genes revealed after two rounds of screens included the previously reported
Recombinant (r)AAV2.5T was selected from the directed evolution of an AAV capsid library in human airway epithelium (HAE). The gene rAAV2.5T is a chimera N-terminal unique coding sequence AAV2 VP1 (VP1u) and VP2- VP3-coding AAV5 with single amino acid mutation A581T. We conducted two rounds genome wide CRISPR gRNA screening for host factors limiting transduction HeLa S3 cells. screen identified several genes that are critical cells, including previously reported KIAA0319L , TM9SF2 VPS51...
Abstract Background: To investigate the characteristic expression of circular RNAs (circRNA) in peripheral blood individuals with latent autoimmune diabetes adults (LADA) and healthy individuals(NG) to identify dysregulated circRNAs that could be used differentiate between LADA individuals. Results: A total 992 differentially expressed were detected Group LA vs NG:209 significantly upregulated 783 downregulated.These distributed on all chromosomes, including sex most derived from exons. Two...