A5057842034- Retinal Diseases and Treatments
- Connexins and lens biology
- Retinal Development and Disorders
- Advanced Glycation End Products research
- Calpain Protease Function and Regulation
- Heat shock proteins research
- Retinal Imaging and Analysis
- Retinopathy of Prematurity Studies
- Pancreatic function and diabetes
- Neuroinflammation and Neurodegeneration Mechanisms
- Muscle Physiology and Disorders
- Retinoids in leukemia and cellular processes
- Adipose Tissue and Metabolism
- S100 Proteins and Annexins
- Cellular transport and secretion
- Glaucoma and retinal disorders
- Neurological Disorders and Treatments
- Biochemical effects in animals
- Neuroscience and Neural Engineering
- PI3K/AKT/mTOR signaling in cancer
- Diabetes and associated disorders
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Retinal and Optic Conditions
- RNA regulation and disease
University of Michigan
2016-2025
W.K. Kellogg Foundation
2013-2023
Michigan United
2016-2021
Michigan Medicine
2021
Visual Sciences (United States)
2017-2021
Laboratoire d’immunologie intégrative du cancer
2021
Ann Arbor Center for Independent Living
2018-2019
Inserm
2002-2015
Centre National de la Recherche Scientifique
2014-2015
Université Claude Bernard Lyon 1
2014-2015
Diabetic retinopathy is characterized by early onset of neuronal cell death. We previously showed that insulin mediates a prosurvival pathway in retinal neurons and normal retina expresses highly active basal receptor/Akt signaling stable throughout feeding fasting. Using the streptozotocin-induced diabetic rat model, we tested hypothesis diabetes diminishes receptor concomitantly with increased diabetes-induced apoptosis. The expression, phosphorylation status, and/or kinase activity...
Plasticity and cortical activation during REM sleep is shown by a subset of hippocampal neurons.
BACKGROUND. In this study, we identified the lipidomic predictors of early type 2 diabetic kidney disease (DKD) progression, which are currently undefined.
The Early Treatment Diabetic Retinopathy Study (ETDRS) and other standardized classification schemes have laid a foundation for tremendous advances in the understanding management of diabetic retinopathy (DR). However, technological optics image analysis, especially optical coherence tomography (OCT), OCT angiography (OCTa), ultra-widefield imaging, as well new discoveries retinal neuropathy (DRN), are exposing limitations ETDRS systems to completely characterize changes diabetes, which we...
Diabetic retinopathy (DR) is a leading cause of blindness in working-age adults and remains an important public health issue worldwide. Here we demonstrate that the expression stimulator interferon genes (STING) increased patients with DR animal models diabetic eye disease. STING has been previously shown to regulate cell senescence inflammation, key contributors development progression DR. To investigate mechanism whereby contributes pathogenesis DR, diabetes was induced STING-KO mice...
The abnormal retinal neurotransmission observed in Duchenne muscular dystrophy (DMD) patients and some genotypes of mice lacking dystrophin has been attributed to altered expression short products the gene. We have investigated potential role Dp71, most abundant C-terminal gene product, electrophysiology. Comparison scotopic electroretinograms (ERG) between Dp71-null wild-type (wt) littermates revealed a normal ERG with no significant changes b-wave amplitude kinetics. Analysis DMD products,...
To test the hypothesis that capacity to develop left ventricular (LV) hypertrophy might diminish with advancing age, we examined hypertrophic response ascending aortic constriction in 3 groups of adult Fischer 344 rats (9 months, 18 and 22 months age). Aortic was created so cross-sectional areas would be same for rats. Four weeks after imposition constriction, there no significant difference peak LV pressure, peak-to-peak mean systolic pressure gradients between ventricle aorta, cardiac...
Abstract The dystrophin‐associated proteins (DAPs) complex consisting of dystroglycan, syntrophin, dystrobrevin, and sarcoglycans in muscle cells is associated either with dystrophin or its homolog utrophin. In rat retina, a similar was found dystrophin‐Dp71 that serves as an anchor for the inwardly rectifying potassium channel Kir4.1 aqueous pore, aquaporin‐4 (AQP4). Here, using immunofluorescence imaging isolated retinal Müller glial co‐immunoprecipitation experiments performed on enriched...
Diabetic retinopathy is the leading cause of blindness in working age persons. Targeted studies have uncovered several components pathophysiology disease without unveiling basic mechanisms. This study describes use complementary proteomic and genomic discovery methods that revealed proteins crystallin superfamily are increased dramatically early diabetic retinopathy. Orthogonal confirmed amplitude up-regulation greater than other changes described so far A detailed time course during...
Diabetes pathology derives from the combination of hyperglycemia and hypoinsulinemia or insulin resistance leading to diabetic complications including neuropathy, nephropathy retinopathy. Diabetic retinopathy is characterized by numerous retinal defects affecting vasculature neuro-retina, but relative contributions loss signaling have never been directly compared. In this study we tested hypothesis that increased glycemic normalization would exert differential effects on cell survival...
α-Crystallins are small heat shock proteins that regulate cellular damage and cell survival. Expression of the crystallin superfamily in retina their role neuronal survival were investigated two animal models diabetes retinal neurons culture.Crystallin expression was assessed streptozotocin-induced Ins2(Akita) diabetic mice using iTRAQ methodology validated immunoblotting. Protein-protein interactions, solubility properties, subcellular localization αA- αB-crystallins further analyzed vivo a...
Abstract The retinal pigment epithelium (RPE) plays an important role in the development of diabetic retinopathy (DR), a leading cause blindness worldwide. Here we set out to explore Akt2 signaling—integral both RPE homeostasis and glucose metabolism—to DR. Using human tissue genetically manipulated mice (including RPE-specific conditional knockout (cKO) knock-in (KI) mice), investigate whether Akts influences DR models eye disease. We found that Akt1 activities were reciprocally regulated...
Neurodegeneration is a central aspect of the early stages diabetic retinopathy, primary ocular complication associated with diabetes. While progress has been made to improve vascular perturbations there are still no treatment options counteract neuroretinal degeneration Our previous work suggested that molecular chaperones α-crystallins could be involved in pathophysiology retinopathy; however, role and regulation remained unknown. In present study, we demonstrated neuroprotective...
BACKGROUND. This study systematically investigated circulating and retinal tissue lipid determinants of human diabetic retinopathy (DR) to identify underlying alterations associated with severity DR.
Our previous study discussed crystallin family induction in an experimental rat model of retinal detachment. Therefore, we attempted to evaluate the role α-crystallin photoreceptor survival detachment, as well its association with intrinsically neuroprotective protein Fas-apoptotic inhibitory molecule 2 (FAIM2). Separation retina and RPE was induced mouse eyes by subretinal injection hyaluronic acid. Retinas were subsequently analyzed for presence αA-crystallin (HSPB4) αB-crystallin (HSPB5)...