A5088904728- Biochemical and Molecular Research
- Plasma Applications and Diagnostics
- HIV/AIDS drug development and treatment
- Adenosine and Purinergic Signaling
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Synthesis and biological activity
- Systemic Lupus Erythematosus Research
- Nanoplatforms for cancer theranostics
- Polyamine Metabolism and Applications
- Synthesis and Characterization of Heterocyclic Compounds
- Enzyme Structure and Function
- Metal complexes synthesis and properties
- Gas Sensing Nanomaterials and Sensors
- Plasma Diagnostics and Applications
- Crystallization and Solubility Studies
- HER2/EGFR in Cancer Research
- Synthesis and Biological Evaluation
- X-ray Diffraction in Crystallography
- Advanced Photocatalysis Techniques
- Chemical Synthesis and Analysis
- Cancer Research and Treatments
- Trypanosoma species research and implications
- Porphyrin Metabolism and Disorders
- Synthesis and Catalytic Reactions
Boise State University
2015-2024
Molecular Research Institute
2020
Cornell University
2004-2014
Portland State University
2001-2005
University of Toronto
2004-2005
Hospital for Sick Children
2004-2005
Solvay (Germany)
2005
Washington State University
2004
Portland VA Medical Center
2002-2003
Oregon Health & Science University
1990-2003
Trust in science and scientists can greatly influence consideration of scientific developments activities. Yet, trust is a nebulous construct based on emotions, knowledge, beliefs, relationships. As we explored the literature regarding discovered that no instruments were available to assess construct, therefore, developed one. Using process data collection from faculty members undergraduate students, field testing, expert feedback, an iterative design, developed, validated, established...
Many bacteria produce extracellular molecules which function in cell-to-cell communication. One of these molecules, autoinducer 2 (AI-2), was first described as an signal produced by Vibrio harveyi to control luciferase expression. Subsequently, a number have been shown possess AI-2 activity their culture supernatants, and bear the luxS gene product, is required for synthesis. In Porphyromonas gingivalis, pfs, encoding 5′-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTA/SAH'ase),...
Abstract: The expression of amphiregulin (AR) in the basal epidermis transgenic mice [keratin 14 promoter AR gene (K14‐ARGE)] has been previously shown to induce an early‐onset and severe skin pathology, with many similarities psoriasis. In this study, it is demonstrated that involucrin enhancer/promoter‐dependent human (INV‐AR) suprabasal also produces a cutaneous psoriasis‐like phenotype. INV‐AR possess limited lifespan scaling, papillomatous, erythematous partial alopecia. mouse...
Nanostructured<italic>g</italic>-C<sub>3</sub>N<sub>4</sub>effectively kills samples of the clinically relevant microorganisms<italic>E. coli</italic>O157:H7 and<italic>S. aureus</italic>under visible radiation.
The coronavirus disease 2019 pandemic has posed severe threats to humans and the geoenvironment. findings of acute respiratory syndrome 2 (Sars-CoV-2) traces in waste water practice disinfecting outdoor spaces several cities world, which can result into entry disinfectants their by-products storm drainage systems subsequent discharge rivers coastal waters, raise issue environmental, ecological public health effects. aims current paper are investigate potential operate as transmission routes...
Immucillin and DADMe-Immucillin inhibitors are tight binding transition state mimics of purine nucleoside phosphorylases (PNP). 5'-Methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) is proposed to form a similar structure as PNP. The companion paper describes modifications the better match features MTAN have led 5'-thio aromatic substitutions that extend inhibition constants femtomolar range (Singh, V., Evans, G. B., Lenz, D. H., Mason, J., Clinch, K., Mee, S., Painter, F.,...
We have discovered a remarkable synergistic antimalarial interaction between rufigallol and the structurally similar compound exifone. The effects were produced in chloroquine-susceptible chloroquine-resistant clones of Plasmodium falciparum. degree potentiation as estimated by standard isobolar analysis was approximately 60-fold for experiments initiated with asynchronous parasites. most pronounced synergism observed synchronized trophozoite-infected erythrocytes, which synergy at least...
Bgp, one of the surface-localized glycosaminoglycan-binding proteins Lyme disease spirochete, Borrelia burgdorferi, exhibited nucleosidase activity. Infection SCID mice with B. burgdorferi strain N40 mutants harboring a targeted insertion in bgp and apparently retaining all endogenous plasmids revealed that Bgp is not essential for colonization immunocompromised mice.
Finding new sources of biologically active compounds for anticancer or antimicrobial therapies remains an area research. Azothioformamides (ATFs) with a 1,3 N=N-C=S heterodiene backbone are class that chelate metals (e.g., Cu) forming stable ATF metal coordination complexes. In this study, ligands were prepared pyrrolidine, piperidine,
Abstract Through a biochemical and genetic approach, we have identified several plant genes encoding methylthioribose (MTR) kinase, an enzyme involved in recycling of methionine through the methylthioadenosine (MTA) cycle. OsMTK1, MTR kinase from rice (Oryza sativa), is 48.6 kD size shows cooperative kinetics with Vmax 4.9 pmol/min K0.5 16.8 μm. are first to be MTA cycle plants. Insertional mutagenesis unique AtMTK gene Arabidopsis (Arabidopsis thaliana) resulted inability plants grow on as...
5'-Methylthioadenosine/S-adenosylhomocysteine (MTA/AdoHcy) nucleosidase is a key enzyme in number of critical biological processes many microbes. This catalyzes the irreversible hydrolysis N(9)-C(1') bond MTA or AdoHcy to form adenine and corresponding thioribose. The role MTA/AdoHcy methylation, polyamine biosynthesis, methionine recycling, bacterial quorum sensing has made it an important antimicrobial drug target. crystal structures Escherichia coli complexed with transition state analog,...
Methylthioadenosine (MTA) is released as a by-product of S-adenosylmethionine (AdoMet)-dependent reactions central to ethylene, polyamine, or phytosiderophore biosynthesis. MTA hydrolysed by methylthioadenosine nucleosidase (MTN; EC 3.2.2.16) into adenine and methylthioribose which processed through the methionine (Met) cycle produce new molecule AdoMet. In deepwater rice, submergence enhances ethylene biosynthesis, in turn influences positive feedback regulation acireductone dioxygenase...
Free-standing, composite hydrogels containing the visible-light responsive metal-free semiconductor graphitic carbon nitride (g-C3N4) as an integral component have been fabricated by direct casting techniques. At 0.67% g-C3N4 loading, intermolecular interactions between particles and PVA polymer chains enhance both mechanical photophysical properties of resulting hydrogels. In contrast, much higher loadings 3.3 or 6.7% resulted in growth average particle size reduction incorporated...
The development of new and effective antiprotozoal drugs has been a difficult challenge because the close similarity metabolic pathways between microbial mammalian systems. 5'-Methylthioadenosine/S-adenosylhomocysteine (MTA/AdoHcy) nucleosidase is thought to be an ideal target for therapeutic drug design as enzyme present in many microbes but not mammals. MTA/AdoHcy (MTAN) irreversibly depurinates MTA or AdoHcy form adenine corresponding thioribose. inhibition MTAN leads buildup toxic...
Lyme disease is the most prevalent tick-borne in USA with highest number of cases (27 444 patients) reported by CDC year 2007, representing an unprecedented 37% increase from previous year. The haematogenous spread Borrelia burgdorferi to various tissues results multisystemic affecting heart, joints, skin, musculoskeletal and nervous system patients. Although can be effectively treated doxycycline, amoxicillin cefuroxime axetil, discovery novel drugs will benefit patients intolerant these...