Coccidiosis represents a serious threat to the poultry industry, affecting production and causing high morbidity, mortality significant costs resulting from treatment prophylaxis. In-feed anticoccidials have been used for decades managing avian coccidiosis were very effective until drug resistance emerged. The use of natural remedies has become promising alternative in combating chickens. Therefore, purpose present study was assess efficiency commercial herbal formula (H), as oral liquid...

Our phytochemical investigation defined the polyphenol, anthocyanin and flavonoid content from methanolic, ethanolic 50% watery extracts fresh pomegranate juice seeds with a spectrophotometric method. The antioxidant capacity was ABTS DPPH methods. We determined polyphenols Folin-Ciocalteu method, anthocyanins method in Hungarian Pharmacopoeia VIIIth edition at Myrtilli fructus monography flavonoids modified of Romanian Pharmacopeia Xth Cynarae folium monography. expressed concentration...

Around 5% of the population world is affected with disease called diabetes mellitus. The main medication insulin; active form insulin monomer, which an instable molecule, because long storage time, or high temperature, can cause monomer to adapt alternative fold, rich in β-sheets, pharmaceutically inactive. aim this study different complexes all cyclodextrin used for pharmaceutical excipients (native cyclodextrin, methyl, hydroxyethyl, hydroxypropyl and sulfobutylether substituted...

Abstract Objective: Methanol appears in relatively high concentrations alcoholic beverages obtained from fermented fruits distillates. These products are traditionally home made many regions Romania and other EU countries. The chronic use of with methanol concentration can be considered a health risk. purpose this work was to measure Romanian region where brandy-type different (plum, apple, pear, grapes), observe if there is type product that contains more than the others. Methods: content...

This study aimed to develop a HPLC/DAD method in order determine and quantify the reduced glutathione (GSH) oxidized (GSSG) levels rat brain. Due presence of thiol group (-SH), GSH can interact with Ellman's reagent (DTNB), which it forms reaction product through level be quantified, using DAD detection system. Chromatographic separation was achieved after derivatization process by mobile phase acetonitrile (A) phosphate buffer (20 mM, pH = 2.5) (B). The compounds interest were detected at...

Artificial sweeteners were introduced in therapy as sugar substitutes for diabetic patients. Nowadays these substances are widely used substitution low calorie drinks and sweets. Most commonly products to date saccharine, cyclamate, aspartame, acesulfam; maximum accepted daily intakes stated each one.A simple reverse phase (RP18) HPLC-UV method with direct detection (196 nm) was developed by us order measure the concentrations of three sweeteners. No sample preparation is required, other...

The aim of this study is to confirm the formation inclusion complexes between bifonazole (BFZ) and different cyclodextrin (CD) derivatives. Bifonazole, an imidazole antifungal derivative,is a very hydrophobic compound, which major drawback in obtaining topical pharmaceutical formulations with optimal bioavailability. Cyclodextrins may increase local drug delivery by enhancing release and/or permeation. binary systems cyclodextrins were prepared two molar ratios physical-mixture methods.The...

Abstract Oxidative stress appears when the amount of free radicals that are formed in a living organism exceed its spin-trapping ability. One most dangerous human body is hydroxyl radical. It can alter several biomolecules, including unsaturated fatty acids; this process known as lipid peroxidation and lead to cell necrosis generation harmful byproducts malondialdehyde, which serves also biomarker oxidative stress. A new HPLC method with visible detection was developed for malondialdehyde...

Abstract Oxidative stress is an imbalance between free radicals or other reactive species and the antioxidant activity of organism. can induce several illnesses such as cardiovascular disease, neurodegenerative disorders, diabetes, cancer, Alzheimer Parkinson. The biomarkers oxidative are used to test injury biomolecules. indicators lipid peroxidation (malondialdehyde, 4-hydroxy- 2-nonenal, 2-propenal, isoprostanes), protein oxidation (carbonylated proteins, tyrosine derivatives), damage...

Offering a systematic and multivariate analysis of the analytical procedure, development validation HPLC methods using Quality by Design approach are in limelight current research trends. A new, experimental design-aided method for fampridine was developed preliminarily validated according to in-force international guidelines linearity, accuracy, robustness precision. The offers high throughput sample analysis, with an elution time 2.9 minutes, signal detection without excipient interference...

Vegetables can contain significant amounts of nitrate and, therefore, may pose health hazards to consumers by exceeding the accepted daily intake for nitrate. Different hydroponic growing patterns were examined in this work order obtain 'nitrate-free lettuces'. Growing lettuces on low content nutrient solution resulted a decrease lettuces' concentrations (1741 versus 39 mg kg(-1)), however beneficial effect was cancelled out an increase ambient temperature. Nitrate replacement with ammonium...

Mefenamic acid (MA) is a widely used non-steroidal antiinflammatory (NSAID) drug. The adverse effects typical of NSAIDs are also present in the case MA, partly due to its low water solubility. aim this study was increase solubility MA order influence absorption and bioavailability. Solid dispersions were prepared by melting method using polyethylene glycol 6000 different types (laurate, D-1216; palmitate, P-1670; stearate, S-1670) amounts sucrose esters as carriers. X-ray diffraction results...

Monosodium glutamate (MSG) is the sodium salt of glutamic acid (GLA), used as a flavour enhancer. MSG considered controversial substance. It incriminated in disturbing antioxidant system, but also has beneficial effects, GLA metabolism plays crucial role homeostasis. This study highlights which positive or negative aspects sub-chronic consumption are better reflected subjects potentially affected by advanced age. Daily doses were administered to four groups two-year-old Wistar rats for 90...

To date, monosodium glutamate is the most used flavour enhancing food additive. Because high levels of are toxic to brain concerns appeared regarding safe use and there a 10 g kg −1 concentration limit in foodstuff. A simple HPLC-UV method, based on derivatization procedure with o -phthaldialdehyde, was developed for determination meat products, soup bases vegetable concentrates. Even if our method less sensitive than HPLC-fluorescence ones widely available, it able measure amounts at least...

Mexiletine (MXL) is a class IB antiarrhythmic agent, acting as non-selective voltage-gated sodium channel blocker, used in therapy racemic mixture R,S-MXL hydrochloride. The aim of the current study was development new, fast, and efficient method for chiral separation MXL enantiomers using capillary electrophoresis (CE) cyclodextrins (CDs) selectors (CSs). After an initial CS screening, several neutral charged CDs, at four pH levels, heptakis-2,3,6-tri-O-methyl-β-CD (TM-β-CD), derivatized...

Abstract Objectives: A simple, quick and low cost HPLC-UV method for assay of meloxicam in plasma pharmaceutical dosage forms was developed. Methods: Separation meloxicam, using a simple reverse phase achieved an Agilent Zorbax SB C18 column, with methanol 1% aqueous solution glacial acetic acid as mobile phase. Elution performed composition gradient, being detected at 355 nm 5 minutes analysis time. The tested on human forms. Results: retention time the 3,7 minutes. Regression showed good...

Chirality is a property of asymmetry which determines the pharmacokinetic and pharmacological profiles optically active pharmaceuticals. Verapamil (VER), calcium channel blocker phenylalkylamine derivative used in treatment cardio-vascular diseases, chiral compound, marketed as racemate, although differences between attributes enantiomers have been reported. The aim our study was to develop new separation method for VER by capillary electrophoresis (CE) using cyclodextrins (CDs) selectors...

Abstract Objective: the number of alkaloids like morphine and codeine found in poppy seeds used food industry are monitored by a directive given European Food Safety Authority. Based on this regulation aim study was to determine quantity from several brands seeds. Methods: an HPLC-UV method (205 nm) developed measure codeine. Sample preparation made using recipes posted Drugs Forum some users. Limits detection were not determined because lowest concentration reference (0.1 μg/ml) detected...

Even if plants can contain high levels of nitrate and nitrite, milk dairy products are not described as sources these anions. The purpose this paper was to measure the concentrations nitrite in forage, cow blood, milk.The results show that ratio plasma is very low, suggesting contamination with unlikely occur. This finding also proves lack an active transport mechanism transfer from blood milk. anion almost disappears through enzymatic conversion; usually μg l−1 were measured.