Plasma protein binding plays a critical role in drug therapy, being key part the characterization of any compound. Among other methods, this process is largely studied by ultrafiltration based on its advantages. However, method also has some limitations that could negatively influence experimental results. The aim study was to underline aspects regarding method, and potential ways overcome them. main are given non-specific substances, effect volume ratio obtained, need rigorous control...

Trimetazidine (TMZ), used for treating stable angina pectoris, has garnered attention in the realm of sports due to its potential performance-enhancing properties, and World Anti-Doping Agency (WADA) classified TMZ on S4 list prohibited substances since 2014. The purpose this narrative mini-review is emphasize biochemical aspects underlying abusive use among athletes as a metabolic modulator cardiac energy metabolism. myocardium’s ability adapt substrate utilization between glucose fatty...

Knowledge of drug pharmacokinetics and tissue distribution is precious for ensuring patient safety optimizing treatments. The varied use local anesthetics, as well the fact that anesthetics can sometimes have low therapeutic indices numerous adverse reactions, makes any novel information valuable. present manuscript describes a pharmacokinetic study ropivacaine carried out after plane block anesthesia on an animal model, using high sensitivity, accurate, precise LC-MS/MS bioanalysis. Both...

Abstract Objective . This study aimed to evaluate the effects of practices and attitudes towards lifestyle in adolescence as risk or protective factors, for both acne occurrence lesions’ severity. Methods A cross-sectional based on a self-reported questionnaire was conducted during 4 months 148 high school students, aged 16-20 years, community Tîrgu Mureș. Acne prevalence severity, demographic anthropometric characteristics, family history vulgaris, smoking behavior weekly intake certain...

This study aimed to quantify the cartilage- and subchondral bone-related effects of low-dose high-dose meloxicam treatment in late phase mono-iodoacetate-induced osteoarthritis stifle.Thirty-four male Wistar rats received intra-articular injection mono-iodoacetate trigger osteoarthritis; 10 control animals (Grp Co) saline. The mono-iodoacetate-injected were assigned three groups treated from week 4 end 7 with placebo P, n = 11), (GrpM Lo, 0.2 mg/kg, 12) or Hi, 1 11) meloxicam. After a period...

The present project aims to evaluate Tribulus terrestris (TT) extracts by addressing various possible mechanisms of action in order see whether the use TT supplements diabetes and complications is justified. Diabetic rats were divided into three groups: diabetic control group, extract with low protodioscin content group (TT-LPC) high (TT-HPC). After twelve weeks treatment, fasting blood glucose, insulin, LH, FSH testosterone levels measured. Both preparations reduced elevated glucose level....

Introduction Benign prostatic hyperplasia (BPH) is a prevalent condition among aging men that affects their life quality due to urinary symptoms. Current pharmacologic treatments, often lead sexual dysfunction, so dietary supplements (DS) containing plant-based compounds such as β-sitosterol (SIT) are preferred. DS highly accessible and widely used, but poorly regulated, patients victims of fraud. The use treat BPH symptoms questionable, this may be not the efficacity active compound...

Antihyperglycemic effects of four extracts obtained from leaves and fruits Vaccinium myrtillus corymbosum were assessed in diabetic rats. In addition, the on diabetic-related complications such as development cataract oxidative stress evaluated. Type 1 diabetes was induced with a single dose streptozotocin Wistar The rats randomly divided into seven equal groups: NC-normal control, DC-diabetic PC-positive control treated metformin, VML-received V. leaf extract, VMLF-received VML fruit...

This study aimed to develop a HPLC/DAD method in order determine and quantify the reduced glutathione (GSH) oxidized (GSSG) levels rat brain. Due presence of thiol group (-SH), GSH can interact with Ellman's reagent (DTNB), which it forms reaction product through level be quantified, using DAD detection system. Chromatographic separation was achieved after derivatization process by mobile phase acetonitrile (A) phosphate buffer (20 mM, pH = 2.5) (B). The compounds interest were detected at...

The mandatory strategy of using internal standard in HPLC is still controversial. Despite that the introduction methodology early stage development technology was used to improve method accuracy and precision, there are practical situations which a simple external quantification adequate. aim study compare determination meloxicam (MXC) human plasma by with or without an according some key points related reason introducing standardization such as reducing sample preparation errors variability...

The purpose of this study was to separate single and multiple pairs six β-blockers enantiomers by high performance liquid chromatography on ovomucoid (OM) column in optimal conditions. separation performed isocratically or gradient elution at 25°C, flow rate 1 mL/min 220 nm. mobile phase consisted phosphate buffer/acetonitrile methanol. effect the organic modifier, influence pH percentage aqueous resolution were investigated. order propranolol (PRP) established as well detection...

In the present study, a HPLC/DAD method was set up to allow for determination and quantification of malondialdehyde (MDA) in brain rodents (rats). Chromatographic separation achieved on Supelcosil LC-18 (3 μm) SUPELCO Column 3.3 cm × 4.6 mm Supelco Saver 0.5 μm filter by using mobile phase acetonitrile (A) phosphate buffer (20 mM, pH = 6) (B). Isocratic elution 14% 86% The injection volume (loop mode) 100 μL with an analysis time 1.5 min. Flow rate at 1 mL/min. eluted compound detected 532...