A5000863537- Adenosine and Purinergic Signaling
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Calcium signaling and nucleotide metabolism
- Lysosomal Storage Disorders Research
- Sphingolipid Metabolism and Signaling
- Endoplasmic Reticulum Stress and Disease
- Receptor Mechanisms and Signaling
- Neuroscience of respiration and sleep
- Neuroinflammation and Neurodegeneration Mechanisms
- Chemokine receptors and signaling
- Biochemical Analysis and Sensing Techniques
- Lipid metabolism and biosynthesis
- Pharmacological Receptor Mechanisms and Effects
- Immune Response and Inflammation
- DNA and Nucleic Acid Chemistry
- Ion Channels and Receptors
- interferon and immune responses
- Nerve injury and regeneration
- Electrolyte and hormonal disorders
- Cytomegalovirus and herpesvirus research
- Neurobiology and Insect Physiology Research
- Adipose Tissue and Metabolism
- Blood disorders and treatments
- PI3K/AKT/mTOR signaling in cancer
Fondation Asile des Aveugles
2024
University of Lausanne
2024
University of Geneva
2009-2023
NCCR Chemical Biology - Visualisation and Control of Biological Processes Using Chemistry
2023
Fondazione Santa Lucia
2003-2010
Sapienza University of Rome
1999-2004
Istituti di Ricovero e Cura a Carattere Scientifico
2003
In animals, adipose tissue contains the main energy store as lipid droplets (LDs) composed of esterified cholesterol (CE) and triacylglycerol (TAG) enveloped in a mono-layer phospholipid decorated by coat proteins. Upon increased demand, dedicated lipases hydrolyse TAG stepwise into free fatty acids that are released circulation made available to peripheral tissue. case aberrant caloric load, TAGs deposited non-adipocyte tissues, primarily liver cells. For instance, non-alcoholic disease...
Abstract The functional expression of the seven‐transmembrane domain G protein‐coupled chemokine receptor CXCR‐4/fusin in rat nerve cell was demonstrated by staining with a polyclonal anti‐CXCR‐4 Ab, and evaluating calcium responses to physiological agonist stromal‐derived factor‐1α (SDF‐1α) both cerebellar granule cells culture Purkinje neurons (PNs) slices. Cerebellar glial, showed pronounced for CXCR‐4. Furthermore, cultured exhibited Ca 2+ transients elicited application SDF‐1α, bodies...
Most cells acquire cholesterol by endocytosis of circulating low-density lipoproteins (LDLs). After cholesteryl ester de-esterification in endosomes, free is redistributed to intracellular membranes via unclear mechanisms. Our previous work suggested that the unconventional phospholipid lysobisphosphatidic acid (LBPA) may play a role modulating flux through endosomes. In this study, we used Prestwick library FDA-approved compounds high-content, image-based screen endosomal lipids, and...
In specialized cell types, lysosome-related organelles support regulated secretory pathways, whereas in nonspecialized cells, lysosomes can undergo fusion with the plasma membrane response to a transient rise cytosolic calcium. Recent evidence also indicates that lysosome secretion be controlled transcriptionally and promote clearance storage diseases. addition, is accumulating low concentrations of cyclodextrins reduce cholesterol-storage phenotype cells animals cholesterol disease...
Neural organoids display three-dimensional (3D) structures that resemble in vivo neural architectures. Previously, we developed a novel two-dimensional (2D) induction-based protocol for culturing spinal cord organoids, enabling size control and recapitulating tube morphogenesis. In this study, evaluated the application of concept to induce anterior regions brain generate human (hANOs). By inducing neuroepithelial (NE) cells 2D re-aggregating them led tube-forming morphogenesis similar...
Cultured cerebellar granule neurons are widely used as a cellular model to study mechanisms of neuronal cell death because they undergo programmed when switched from culture medium containing 25 mM one 5 K + . We have found that the growth-related gene product β (GROβ) partially prevents -depletion-induced death, and neuroprotective action GROβ on cells is mediated through α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) type ionotropic glutamate receptors. GROβ-induced survival was...
Abstract P2X receptors are a family of seven (P2X 1–7 ) cation channels gated by extracellular ATP, widely expressed in neurons and nonneuronal cells. Lipid rafts cholesterol/sphingolipid‐rich membrane domains, involved many cellular processes, including transmembrane receptor signaling, vesicle traffic, protein sorting. We provide direct biochemical evidence that 3 localizes into lipid rafts, primary cultures cerebellar granule as well brain dorsal root ganglia extracts. show exhibits all...
P2X receptors mediate a variety of physiological actions, including smooth muscle contraction, neuro-endocrine secretion and synaptic transmission. Among receptors, the P2X(3) subtype is expressed in sensory neurons dorsal root- trigeminal-ganglia, where it performs well-recognized role pain Recent evidence indicates that strength P2X(3)-mediated responses modulated vivo by altering number at plasma membrane. In present study, we investigate trafficking properties receptor transfected HEK293...
It is well established that several cell surface receptors interact with each other to form dimers and oligomers, which are essential for their activation. Since little known about the quaternary structure of P2Y receptors, in present work, we investigated expression G-protein-coupled P2Y₄ subunit as monomeric or higher-order complex protein. We examined both endogenously expressed subtype aid specific anti-P2Y₄ antiserum, heterologously transfected P2Y₄-tagged use antitag antibodies. In...
Abstract Background In the CNS, several P2 receptors for extracellular nucleotides are identified on neurons and glial cells to participate neuron-neuron, glia-glia glia-neuron communication. Results this work, we describe cellular subcellular presence of metabotropic P2Y 1 receptor in rat cerebellum at two distinct developmental ages, by means immunofluorescence-confocal electron microscopy as well western blotting direct membrane separation techniques. At postnatal day 21, find that...
The growth-related gene product β (GROβ) is a small chemoattractant cytokine that belongs to the CXC chemokine family, and GROβ receptors are expressed in brain, including cerebellum. We demonstrate rat cerebellar granule neurones express receptor CXCR2. also show that, addition known stimulation of phosphoinositide-specific phospholipase C, activates both neutral (N-) acidic (A-) sphingomyelinases (SMase) stress-activated c-Jun N-terminal kinase 1 (JNK1). Although exogenous ceramide...
N-Glycosylation affects the function of ion channels at level multisubunit assembly, protein trafficking, ligand binding and channel opening. Like majority membrane proteins, ionotropic P2X receptors for extracellular ATP are glycosylated in their moiety. Here, we used site-directed mutagenesis to four predicted N-glycosylation sites P2X3 receptor (Asn139, Asn170, Asn194 Asn290) performed comparative analysis role N-glycans on stability, plasma delivery, trimer formation inward currents. We...
ABSTRACT Most cells acquire cholesterol by endocytosis of circulating LDLs. After cholesteryl ester de-esterification in endosomes, free is redistributed to intracellular membranes via unclear mechanisms. Our previous work suggested that the unconventional phospholipid lysobisphosphatidic acid (LBPA) may play a role modulating flux through endosomes. In this study, we used Prestwick library FDA-approved compounds high content, image-based screen endosomal lipids, and LDL-derived cholesterol....
ABSTRACT In specialized cell types, lysosome-related organelles support regulated secretory pathways, while in non-specialized cells, lysosomes can undergo fusion with the plasma membrane response to a transient rise cytosolic calcium. Recent evidence also indicates that lysosome secretion be controlled transcriptionally and promote clearance storage diseases. addition, is accumulating low concentrations of cyclodextrins reduce cholesterol phenotype cells animals disease Niemann-Pick type C,...