A5083861783- Acute Myeloid Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- CAR-T cell therapy research
- Immune cells in cancer
- Retinoids in leukemia and cellular processes
- Histone Deacetylase Inhibitors Research
- Immune Cell Function and Interaction
- Genetic Neurodegenerative Diseases
- Hereditary Neurological Disorders
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Apelin-related biomedical research
- Mitochondrial Function and Pathology
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Hematological disorders and diagnostics
- Epigenetics and DNA Methylation
- Erythrocyte Function and Pathophysiology
- Animal Genetics and Reproduction
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cancer Research and Treatments
- Blood Coagulation and Thrombosis Mechanisms
Institute of Cytology
2020-2024
Federal Almazov North-West Medical Research Centre
2020-2021
Academy of Medical Sciences
2014
Russian Academy of Sciences
2014
The tumor microenvironment (TME) plays an essential role in progression and modulating response to anticancer therapy. Cellular senescence leads a switch the cell secretome, characterized by senescence-associated secretory phenotype (SASP), which may regulate tumorigenesis. Senolytic therapy is considered novel strategy that eliminates deleterious effects of senescent cells TME. Here, we show two different types senolytic drugs, despite efficiently depleting cells, have opposite on...
С помощью ПЦР в режиме реального времени проведен анализ уровня мРНК генов центаурина бета 5 (ACAP3) и цистатина Б (CSTB) лейкоцитах периферической крови у здоровых людей, а также пациентов с симптоматической эпилепсией болезнью Паркинсона (форма ранним дебютом). По сравнению контролем обнаружено повышение гена ACAP3 мужчин женщин (в 2,5- 5-раз соответственно), (примерно 6 раз). Кроме того, наблюдалось снижение CSTB при эпилепсии 3 раза) болезни 2.5 раза). Полученные данные указывают на...
Background . Myelodysplastic syndrome is a group of malignant blood diseases with high risk transformation into acute myeloid leukemia. One treatment approach to target immune checkpoints (ICs) that are overexpressed on tumor cells. To develop these drugs, relevant models needed for highthroughput screening and study biologically active substances, since traditionally used (mouse patient biomaterials) difficult access, financially laborintensive, characterized by poorly reproducible results....
Aim. To identify mutations in IDH1/IDH2, DNMT3A, and ASXL1 genes responsible for genome epigenetic regulation their co-occurrence with FLT3, NPM1, RUNX1 newly diagnosed adult acute myeloid leukemias (AML). Materials & Methods. The study included 56 patients AML treated at the VA Almazov National Medical Research Center. Among them there were 34 men и 22 women aged 18-76 years (median 46 years). Mutation status of IDH1, IDH2, was assessed by Sanger sequencing method. Molecular genetic...
Background. FLT3 gene is an important prognostic molecular marker in acute myeloid leukemia (AML). However, the detection of mutations presents a challenge. Aim. To compare techniques used for mutations, and to develop test-system based on polymerase chain reaction (PCR) quick reliable determination mutation status. Materials & Methods. Bone marrow samples obtained from AML patients were subjected examination. detect FLT3-ITD FLT3-TKD PCR was performed with subsequent agarose gel...
Recently wild-type p53-induced phosphatase was implicated in the pathogenesis of acute myeloid leukemia (AML) and “pre-leukemia” myeloproliferative conditions. Here we decided to check how strategy directed inhibition affected sensitivity conventional chemotherapy. All experiments were conducted on AML cell lines cultivated vitro. The levels vary different lines. chemical compound GSK2830371 reduced diminished its activity. did not significantly change cycle distribution cells when used...
Background. Up to the present survival rates of acute myeloid leukemia (AML) patients have remained low. A successful AML management presupposes generating personalized models disease. The most promising research activity in this field is creation patient-derived xenograft using advanced strain immunodeficient humanized NSG-SGM3 mice. Aim. To generate Materials & Methods. PDX was based on bone marrow aspirates taken from 4 with newly diagnosed who were treated at V.A. Almazov National...
NK-cells as innate immunity elements manifest key reactions of antitumor immune response. NKG2D is an activating transmembrane receptor which responsible for cytotoxicity initiation in response to the binding specific ligands genetically modified cells. Selective expression provides a unique perspective on therapy wide variety tumors. Acute myeloid leukemias (AML) are malignant hematological tumors with high relapse risk. Due complexity AML treatment strategy it necessary develop new...