A5048766557- Bone Metabolism and Diseases
- Cannabis and Cannabinoid Research
- Cytokine Signaling Pathways and Interactions
- Bone health and treatments
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Neuropeptides and Animal Physiology
- Pancreatic function and diabetes
- Estrogen and related hormone effects
- Parkinson's Disease Mechanisms and Treatments
- Chemokine receptors and signaling
- RNA Interference and Gene Delivery
- Forensic Toxicology and Drug Analysis
- Neuroscience and Neuropharmacology Research
- interferon and immune responses
- Bone and Joint Diseases
- Nerve injury and regeneration
- Cancer Genomics and Diagnostics
- Inflammatory mediators and NSAID effects
- Protease and Inhibitor Mechanisms
- Prostate Cancer Treatment and Research
- Advanced Proteomics Techniques and Applications
- Cancer Research and Treatments
- RNA modifications and cancer
- Extracellular vesicles in disease
Göteborgs Stads
2024
University of Gothenburg
2019-2024
Umeå University
2009-2021
Sahlgrenska University Hospital
2019
Uppsala University
1998-2018
Lund University
2016-2017
MRC Human Nutrition Research
2008
University of Gävle
2005
Science for Life Laboratory
2002
Abstract IL-6, leukemia inhibitory factor (LIF), and oncostatin M (OSM) are IL-6-type cytokines that stimulate osteoclast formation function. In the present study, resorptive effects of these agents their regulation receptor activator NF-κB ligand (RANKL), RANK, osteoprotegerin (OPG) were studied in neonatal mouse calvaria. When tested separately, neither human (h) IL-6 nor soluble IL-6R (shIL-6R) stimulated bone resorption, but when hIL-6 shIL-6R combined, significant stimulation both...
Interleukin (IL)-4 and IL-13 are cytokines that inhibit bone resorption. Data showing an inhibitory effect of IL-4 on RANK mRNA in mouse calvariae were first reported at the 22nd American Society for Bone Mineral Research Meeting (Lerner, U.H., Conaway, H. 2000) J. Min. Res. 15, Suppl. 1, Abstr. SU 230). In present study, release 45Ca from cultured calvarial bones stimulated by different cytokines, peptides, steroid hormones was inhibited IL-13. decreased receptor activator nuclear factor-κB...
In Parkinson's disease and other Lewy body disorders, the propagation of pathology has been accredited to spreading extracellular α-synuclein (α-syn). Although pathogenic mechanisms are not fully understood, cell-to-cell transfer α-syn via exosomes vesicles (EVs) reported. Here, we investigated whether altered molecular properties can influence distribution secretion in human neuroblastoma cells. Different variants, including α-syn:hemi-Venus disease-causing mutants, were overexpressed EVs...
Skeletal metastatic disease is a deleterious consequence of dissemination tumor cells from numerous primary sites, such as prostate, lung and breast. metastases are still incurable, resulting in development clinical complications decreased survival for cancer patients with disease. During the last decade, cell-derived microvesicles have been identified suggested to be involved progression. Whether exosomes bone cell interactions site still, however, rather unexplored field. Here we show that...
Two compounds, URB602 and URB754, have been reported in the literature to be selective inhibitors of monoacylglycerol lipase, although a recent study has questioned their ability prevent 2-arachidonoyl hydrolysis by brain homogenates cerebellar membranes. In present study, these compounds inhibit lipase fatty acid amide hydrolase reinvestigated.Homogenates cell lines were incubated with test and, thereafter, either [(3)H]-2-oleoylglycerol or [(3)H]-anandamide. Labelled reaction products...
Background Recent data have indicated that there may be a dysregulation of endocannabinoid metabolism in cancer. Here we investigated the expression metabolising enzyme fatty acid amide hydrolase (FAAH) well characterised tissue microarray from patients diagnosed with prostate cancer at transurethral resection for voiding problems. Methodology/Principal Findings FAAH immunoreactivity (FAAH-IR) was assessed formalin-fixed paraffin-embedded non-malignant and tumour cores 412 CB1 receptor...
// Emelie Englund 1 , Giacomo Canesin 2, * Konstantinos S. Papadakos 1, Neelanjan Vishnu 3 Emma Persson Bart Reitsma Aseem Anand 2 Laila Jacobsson Leszek Helczynski Hindrik Mulder Anders Bjartell and Anna M. Blom Department of Translational Medicine, Division Medical Protein Chemistry, Lund University, Malmö, Sweden Urological Cancers, Clinical Sciences Unit Molecular Metabolism, These authors have contributed equally to this work Correspondence to: Blom, email: anna.blom@med.lu.se Keywords:...
Tumor cells interact with the microenvironment that specifically supports and promotes tumor development. Key components in environment have been linked to various aggressive cancer features can further influence presence of subpopulations specific functions, including stem migratory cells. To model understand microenvironments we developed an experimental platform using cell-free patient-derived scaffolds (PDSs) from primary breast cancers infiltrated standardized cell lines. This PDS...
Increased vitamin A (retinol) intake has been suggested to increase bone fragility. In the present study, we investigated effects of retinoids on resorption in cultured neonatal mouse calvarial bones and their interaction with glucocorticoids (GC). All-trans-retinoic acid (ATRA), retinol, retinalaldehyde, 9-cis-retinoic stimulated release 45Ca from bones. The resorptive effect ATRA was characterized by mRNA expression genes associated osteoclast differentiation, enhanced number, matrix...
Whether vitamin A promotes skeletal fragility, has no effect on fracture rate, or protects against bone loss is unclear. In the present study, effects of retinoids osteoclast differentiation in cultured mouse marrow cells (BMCs), macrophages (BMMs), spleen cells, and RAW264.7 were evaluated by analyzing formation expression genes important signal transduction function. All-trans-retinoic acid (ATRA) did not stimulate osteoclastogenesis BMCs, but inhibited hormone RANKL-induced gene...
The cysteine proteinase inhibitor cystatin C inhibited RANKL-stimulated osteoclast formation in mouse bone marrow macrophage cultures, an effect associated with decreased mRNA expression of Acp5, Calcr, Ctsk, Mmp9, Itgb3, and Atp6i, without on proliferation or apoptosis. effects were concentration dependent half-maximal inhibition at 0.3 μM. Cystatin also when osteoclasts cultured bone, leading to resorption pits. cells retained characteristics phagocytotic macrophages cotreated C. Three...
Breast cancer tumors display different cellular phenotypes. A growing body of evidence points towards a population stem cells that is important for metastasis and treatment resistance, although the characteristics these are incomplete. We used mammosphere formation assay label-retention as functional approaches to enrich with degree cell properties in breast line MDA-MB-231 performed single-cell RNA sequencing. clustered based on their gene expression profiles identified three...
The aggregation of alpha-synuclein (αSyn) is the pathological hallmark Parkinson's disease, dementia with Lewy bodies and related neurological disorders. However, physiological function protein how this relates to its effects remain poorly understood. One proposed roles αSyn promote soluble N-ethylmaleimide-sensitive factor activating receptor (SNARE) complex assembly by binding VAMP-2. objective study was visualize co-localization between SNARE proteins (VAMP-2, SNAP-25 syntaxin-1) for...
Osteoclast formation is controlled by stromal cells/osteoblasts expressing macrophage colony-stimulating factor (M-CSF) and receptor activator of NF-κB ligand (RANKL), crucial for osteoclast progenitor cell proliferation, survival differentiation, osteoprotegerin (OPG) that inhibits the interaction between RANKL its RANK. Recent data have strongly indicated nervous system plays an important role in bone biology. In present study, effects neuropeptide vasoactive intestinal peptide (VIP),...
Background and Purpose: The gp130 family of cytokines signals through receptors dimerizing with the subunit. Downstream signaling typically activates STAT3 but also SHP2/Ras/MAPK pathways. Oncostatin M (OSM) is a unique cytokine in this since receptor (OSMR) non-redundant pathway by recruitment adapter Shc1. We have studied functional relevance Shc1 for OSM-induced bone resorption. Experimental Approach: Osteoblasts were stimulated OSM activations using real-time PCR Western blots. role...
Background The endocannabinoid ligand anandamide (AEA) is removed from the extracellular space by a process of cellular uptake followed metabolism. In many cells, such as RBL-2H3 cell line, inhibition FAAH activity reduces observed uptake, indicating that enzyme regulates controlling intra- : AEA concentration gradient. However, in other FAAH-expressing no effect seen. It not clear, however, whether these differences are methodological nature or due to properties cells themselves....
It is well known that tumour cells are dependent on communication with the microenvironment. Previously, it has been shown hypoxia (HX) induces pronounced, diverse and direct effects cancer stem cell (CSC) qualities in different breast subtypes. Here, we describe mechanism by which HX-induced secretion influences spreading of CSCs. Conditioned media (CM) from estrogen receptor (ER)-α-positive hypoxic cultures increased fraction CSCs compared to normal growth conditions, as determined using...
Dosage-dependent release of 45Ca was observed from prelabeled mouse calvarial bones after treatment with two thiazolidinediones, troglitazone and ciglitazone. Release by ciglitazone decreased the osteoclast inhibitors acetazolamide, calcitonin, 3-amino-1-hydroxypropylidene-1,1-bisphosphonate, IL-4, but not affected peroxisome proliferator-activated receptor gamma antagonist, GW 9662, mitotic inhibitor, hydroxyurea, or indomethacin. Enhanced expression activator nuclear factor-kappaB ligand...