A5043428039- Bone Metabolism and Diseases
- Oral microbiology and periodontitis research
- Neuropeptides and Animal Physiology
- Dental Implant Techniques and Outcomes
- NF-κB Signaling Pathways
- Immune Response and Inflammation
- Periodontal Regeneration and Treatments
- Bone health and treatments
- Oral Health Pathology and Treatment
- Phagocytosis and Immune Regulation
- Cytokine Signaling Pathways and Interactions
- Chemokine receptors and signaling
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- S100 Proteins and Annexins
- Connective tissue disorders research
- Cell Adhesion Molecules Research
- Osteomyelitis and Bone Disorders Research
- Dental Health and Care Utilization
- Oral and gingival health research
- IL-33, ST2, and ILC Pathways
- Regulation of Appetite and Obesity
- Inflammatory mediators and NSAID effects
- Hypothalamic control of reproductive hormones
- Peptidase Inhibition and Analysis
- dental development and anomalies
Umeå University
2015-2025
American Academy of Arts and Sciences
2022
University of Copenhagen
2022
University of Bergen
2022
University of Turku
2022
Garvan Institute of Medical Research
2006-2007
St Vincent's Hospital
2007
Science for Life Laboratory
1999-2002
Abstract Tissue nonspecific alkaline phosphatase (TNAP) knockout (ko) mice manifest defects in bone mineralization that mimic the phenotypic abnormalities of infantile hypophosphatasia. In this article, we have searched for differences between calvarial osteoblasts and osteoclasts wild-type (wt), heterozygous homozygous TNAP null mice. vitro release of45Ca from bones, with without stimulation parathyroid hormone (PTH), revealed no functional difference three genotypes. Studies primary...
The importance of neuropeptide Y (NPY) and Y2 receptors in the regulation bone energy homeostasis has recently been demonstrated. However, contributions other are less clear. Here we show that Y1 expressed on osteoblastic cells. Moreover, adipose tissue mass elevated Y1(-/-) mice with a generalized increase formation cortical cancellous surfaces. Importantly, inhibitory effects NPY marrow stromal cells vitro absent derived from mice, indicating direct action via this receptor. Interestingly,...
Periodontal Ehlers-Danlos syndrome (pEDS) is an autosomal-dominant disorder characterized by early-onset periodontitis leading to premature loss of teeth, joint hypermobility, and mild skin findings. A locus was mapped approximately 5.8 Mb region at 12p13.1 but no candidate gene identified. In international consortium we recruited 19 independent families comprising 107 individuals with pEDS identify the locus, characterize clinical details in those defined genetic causes, try understand...
Interleukin (IL)-4 and IL-13 are cytokines that inhibit bone resorption. Data showing an inhibitory effect of IL-4 on RANK mRNA in mouse calvariae were first reported at the 22nd American Society for Bone Mineral Research Meeting (Lerner, U.H., Conaway, H. 2000) J. Min. Res. 15, Suppl. 1, Abstr. SU 230). In present study, release 45Ca from cultured calvarial bones stimulated by different cytokines, peptides, steroid hormones was inhibited IL-13. decreased receptor activator nuclear factor-κB...
Germ line or hypothalamus-specific deletion of Y2 receptors in mice results a doubling trabecular bone volume. However, the specific mechanism by which increases mass has not yet been identified. Here we show that cultured adherent marrow stromal cells from <i>Y2</i><sup>-/-</sup> also demonstrate increased mineralization <i>in vitro</i>. Isolation two populations progenitor cell types, an immature mesenchymal stem population and more highly differentiated cells, revealed greater number...
Background Interleukin-34 (IL-34) is a recently discovered cytokine functionally overlapping macrophage colony stimulating factor (M-CSF), mediator of inflammation and osteoclastogenesis in bone-degenerative diseases such as rheumatoid arthritis. The objective this study was to assess the expression IL-34 human gingival fibroblasts investigate if pro-inflammatory cytokines tumor necrosis alpha (TNF-α) Interleukin-1Β (IL-1β) modulate its expression, moreover could contribute recruitment...
This randomized clinical trial aimed at comparing radiological, and microbial effects of surgical treatment peri-implantitis alone or in combination with enamel matrix derivative (EMD).Twenty-six subjects were treated open flap debridement decontamination the implant surfaces gauze saline preceding adjunctive EMD no EMD. Bone level (BL) change was primary outcome secondary outcomes changes pocket depth (PD), plaque, pus, bleeding microbiota peri-implant biofilm analyzed by Human Oral Microbe...
Normal bone homeostasis, which is regulated by bone-resorbing osteoclasts and bone-forming osteoblasts perturbed inflammation. In chronic inflammatory disease with disturbed remodelling, e.g. rheumatoid arthritis, patients show increased serum levels of the chemokine eotaxin-1 (CCL11). Herein, we demonstrate an driven expression CCL11 in tissue a novel role osteoclast migration resorption. Using lesion model primary cell cultures, discovered that express vivo vitro during conditions....
Periodontitis is a chronic inflammatory disease of tooth supporting tissues resulting in periodontal tissue destruction, which may ultimately lead to loss. The characterized by continuous leukocyte infiltration, likely mediated local chemokine production but the pathogenic mechanisms are not fully elucidated. There no reliable serologic biomarkers for diagnosis periodontitis, today based solely on degree and there available biological treatment tool. Prompted increasing interest...
Abstract Objective To assess the clinical and radiographic outcomes 3 5 years after surgical treatment of peri‐implantitis per se or in combination with an enamel matrix derivative ( EMD ). Materials Methods At baseline, 29 patients were randomized to adjunctive no . One year peri‐implantitis, 25 remained eligible for survival analyses at 3‐ 5‐year follow‐up. The primary implant loss bone level BL ) change measured on radiographs, secondary outcomes, bleeding probing, pus plaque each...
Interleukin-6 (IL-6)-type cytokines are pleiotropic molecules capable of stimulating bone resorption and expressed by numerous cell types. In the present study, we tested hypothesis that gingival fibroblasts may exert local osteotropic effects through production IL-6 related cytokines. IL-6-type cytokine expression regulation IL-1β tumor necrosis factor-α (TNF-α) were studied in from non-inflamed gingiva healthy individuals. Constitutive mRNA IL-6, IL-11, leukemia inhibitory factor (LIF),...
To describe the biology of alveolar bone regeneration.Four comprehensive reviews were performed on (a) mesenchymal cells and differentiation factors leading to formation; (b) critical interplay between resorbing formative cells; (c) role osteoimmunology in formation maintenance bone; (d) self-regenerative capacity following injury or tooth extraction prepared prior workshop.This summary information adds fuller understanding regenerative response with implications reconstructive procedures...
The effects of calcitonin (CT) on osteoclast formation and gene expression have been studied in cultured mouse spleen cells bone marrow macrophages (BMMs). CT inhibited the multinucleated osteoclasts resorption pits cell cultures BMM as well CD115(+) CD3(-) CD45R(-)sorted cultures, incubated presence macrophage colony-stimulating factor receptor activator NF-kappaB ligand (RANKL). No effect apoptosis by was observed. did not affect mRNA expressions RANK c-Fms, or a wide variety transcription...
Three distinct complementary DNAs for vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating (PACAP) receptors have been cloned designated VIP-1 receptor (VIP-1R), VIP-2 (VIP-2R), PACAP (PACAP-R). In the present study, we characterized binding sites on primary mouse calvarial osteoblasts VIP related peptides. By analyzing cAMP response, rank order of response observed was 38 > 27 helodermin helospectin glucagon PHI >>> secretin. The VIP-2R/PACAP-R antagonist, 6–38,...
The expressions of the calcitonin receptor (CTR), receptor-like (CLR), activity-modifying proteins (RAMP) 1-3, and component protein (RCP) have been studied in mouse bone marrow macrophages (BMM) during osteoclast differentiation, induced by treatment with M-CSF RANKL. Analyses mRNA showed that CLR RAMP1-3, but not CTR, were expressed stimulated BMM. RANKL gradually increased CTR mRNA, transiently enhanced decreased RAMP1 did affect RAMP2, RAMP3, or RCP mRNA. However, levels RAMP1-3 as...
The osteotropic interleukin-6 (IL-6) types of cytokines IL-6, IL-11, and leukemia inhibitory factor (LIF), but not oncostatin M, are expressed by human gingival fibroblasts, their expressions regulated IL-1 tumor necrosis factor-α (TNF-α). In the present study, we investigated whether signaling through Toll-like receptor 2 (TLR2) can affect expression these in fibroblasts. Lipopolysac-charide (LPS) from P. gingivalis was found to stimulate IL-6 LIF mRNA protein, IL-11 or OSM mRNA. Using two...
Here, we investigated whether the cell surface glycoprotein CD47 was required for normal formation of osteoblasts and osteoclasts to maintain bone activity in vitro vivo. In parathyroid hormone or 1α,25(OH)2-vitamin D3 (D3)-stimulated marrow cultures (BMC) from CD47(-/-) mice, found a strongly reduced multinuclear tartrate-resistant acid phosphatase (TRAP)(+) osteoclasts, associated with expression osteoclastogenic genes (nfatc1, Oscar, Trap/Acp, ctr, catK, dc-stamp). The production M-CSF...
Introduction Periodontitis is associated with rheumatoid arthritis (RA). One hypothesis posits that this connection arises from the formation of autoantibodies against citrullinated proteins (ACPA) in inflamed gums, possibly triggered by Porphyromonas gingivalis . We previously demonstrated an increased antibody response to P. arginine gingipains (anti-Rgp IgG), not only individuals severe periodontitis compared controls, but RA versus association ACPA. In present study, we set out further...