A5012528125- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Diabetes and associated disorders
- Infant Nutrition and Health
- Pancreatic function and diabetes
- Bacterial biofilms and quorum sensing
- Oral microbiology and periodontitis research
- Periodontal Regeneration and Treatments
- Immune Cell Function and Interaction
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Animal Genetics and Reproduction
- Diet, Metabolism, and Disease
- Adipokines, Inflammation, and Metabolic Diseases
- Oral Health Pathology and Treatment
- Diabetes Management and Research
- Autoimmune and Inflammatory Disorders Research
- Bone health and treatments
- Metabolism and Genetic Disorders
- Dental Implant Techniques and Outcomes
- Fatty Acid Research and Health
- Oral and gingival health research
- Chemokine receptors and signaling
- Hormonal Regulation and Hypertension
Umeå University
2011-2024
Inserm
2008
Institut de Neurobiologie de la Méditerranée
2008
Centre de Recherche en Nutrition Humaine d'Auvergne
2008
Pediatrics and Genetics
2002
Institut für Medizinische Biometrie, Informatik und Epidemiologie
1993
University of Bonn
1993
Citrobacter freundii encodes an inducible chromosomal beta-lactamase. Induction requires the product of ampR gene, which is transcribed in opposite orientation from ampC beta-lactamase gene. We show here that AmpR protein acts as a transcriptional activator by binding to DNA region immediately upstream promoter. The DNase I footprint pattern was not affected growth presence beta-lactam inducer or use extracts prepared cells carrying ampD2 allele leading semiconstitutive production It...
In Citrobacter freundii and Enterobacter cloacae, synthesis of AmpC beta-lactamase is inducible by the addition beta-lactams to growth medium. Spontaneous mutants that constitutively overproduce enzyme occur at a high frequency. When C. ampC gene cloned into Escherichia coli together with regulatory ampR, expression from clone inducible. cefotaxime-resistant were selected an E. strain carrying ampR genes on plasmid. Virtually all isolates had chromosomal mutations leading semiconstitutive...
CEL-maturity onset diabetes of the young (MODY), with pancreatic lipomatosis and exocrine dysfunction, is due to dominant frameshift mutations in acinar cell carboxyl ester lipase gene (CEL). As Cel knock-out mice do not express phenotype mutant protein has an altered intrinsically disordered tandem repeat domain, we hypothesized that disease mechanism might involve a negative effect protein. In silico analysis showed pI was markedly increased from pH 3.3 wild-type (WT) 11.8 (MUT) human CEL....
Summary The chromosomal ampC β‐lactamase in Citrobacter freundii and Enterobacter cloacae is inducible by β‐lactam antibiotics. When an gene introduced on a plasmid into Escherichia coli together with its transcriptional regulator ampR , the plasmid‐borne still inducible. We have isolated mutants, containing alterations novel E. gene, ampG which cloned C. unable to respond inducers. was cloned, sequenced mapped minute 9.6 chromosome. deduced amino acid sequence predicted AmpG be 53kDa,...
Summary The cloned Citrobacter freundii ampC β‐lactamase is inducible in the presence of its regulatory gene ampR Escherichia coli (Lindberg et al. , 1985). basal level expression and inducibility are affected by two E. proteins encoded closely linked ampD ampE genes. Deletion both genes led to constitutive ampR‐dependent overproduction β‐lactamase, whereas an out‐of‐frame deletion AmpD caused increase twofold. This ampD1 mutant was at lower β‐lactam concentrations than wild type. An IS1...
ABSTRACT A wide range of pathogens, including human immunodeficiency virus type 1 (HIV-1), hepatitis C virus, Ebola cytomegalovirus, dengue Mycobacterium , Leishmania and Helicobacter pylori can interact with dendritic cell (DC)-specific ICAM3-grabbing nonintegrin (DC-SIGN), expressed on DCs a subset B cells. More specifically, the interaction gp120 envelope protein HIV-1 DC-SIGN facilitate transfer to CD4 + T lymphocytes in trans enhance infection. We have previously demonstrated that...
In rodents, bile salt-stimulated lipase (BSSL) and pancreatic lipase-related protein 2 (PLRP2) are the dominant lipases expressed in exocrine pancreas early life when milk is main food. The aim of present study was to evaluate whether BSSL PLRP2 also key enzymes neonatal intestinal fat digestion. Using Caco-2 cells as a model for small epithelium, purified human were incubated apical compartment with substrates, salt composition concentrations physiologic newborn infants. Both hydrolyzed...
Introduction Periodontitis is associated with rheumatoid arthritis (RA). One hypothesis posits that this connection arises from the formation of autoantibodies against citrullinated proteins (ACPA) in inflamed gums, possibly triggered by Porphyromonas gingivalis . We previously demonstrated an increased antibody response to P. arginine gingipains (anti-Rgp IgG), not only individuals severe periodontitis compared controls, but RA versus association ACPA. In present study, we set out further...
The amp operon, which is located on the Escherichia coli chromosome, modulates induction of plasmid-borne beta-lactamase genes by extracellular beta-lactam antibiotics. This suggests that gene products AmpD and AmpE may function in transduction external signals. beta-Lactam antibiotics are analogs cell wall components can be released during morphogenesis enterobacteria. operon was studied to determine its importance signal morphogenesis. peptidoglycan compositions mutants were determined...
Bile salt-stimulated lipase (BSSL) is a lipolytic digestive enzyme with broad substrate specificity secreted from exocrine pancreas into the intestinal lumen in all species and lactating mammary gland milk of some species, notably humans but not cows. BSSL breast facilitates digestion absorption fat promotes growth small for gestational age preterm infants. Thus, purified recombinant human (rhBSSL) can be used treatment patients malabsorption expressing rhBSSL transgenic cloned cows would...
Amyotrophic lateral sclerosis (ALS) is currently an incurable fatal motor neuron syndrome characterized by progressive weakness, muscle wasting and death ensuing 3–5 years after diagnosis. Neurotrophic factors (NTFs) are known to be important in both nervous system development maintenance. However, the attempt translate potential of NTFs into therapeutic options remains limited despite substantial number approaches, which have been tested clinically. Using quantitative RT-PCR (qRT-PCR)...
The ampD gene product regulates the expression of AmpC beta-lactamase in gram-negative bacteria and is proposed to be involved peptidoglycan metabolism. In this study, we sequenced wild type three mutant genes Enterobacter cloacae Citrobacter freundii. They exhibited a high degree homology with corresponding Escherichia coli except carboxy termini, where, wild-type E. C. freundii, four additional amino acids yielding Ser-X-X-Lys motif were found. Evidence that C-terminal region necessary for...
Objective Dendritic cells bind an array of antigens and DC-SIGN has been postulated to act as a receptor for mucosal pathogen transmission. Bile salt-stimulated lipase (BSSL) from human milk potently binds blocks mediated trans-infection CD4+ T-lymphocytes with HIV-1. was study variation in binding properties the relation between capacity BSSL gene polymorphisms. Study Design ELISA PCR were used exon 11 size derived 269 different mothers distributed over 4 geographical regions. Results...
The sequalae of periodontitis include irreversible degradation tooth-supporting structures and circulatory spread inflammatory mediators. However, the serum protein profile in is not well described, which partly attributable to limited number studies based on large well-characterized cohorts. This study aims identify novel, circulating inflammation-related proteins associated with within PerioGene North case-control study, includes 478 cases severe 509 periodontally healthy controls....
The apparent molecular mass of human milk bile salt‐stimulated lipase (BSSL) varies between mothers. basis for this is unknown, but indirect evidence has suggested the differences to reside in a region repeats located C‐terminal part protein. We here report that polymorphism within exon 11 BSSL gene explanation variants found milk. By Southern blot hybridization we analyzed from mothers known have different masses their A␣polymorphism was 11, previously shown consist 16 near identical 33 bp...
Objective The present study aimed to explore the hypothesis that bile salt-stimulated lipase (BSSL), in addition being a key enzyme dietary fat digestion during early infancy, plays an important role inflammation, notably arthritis. Methods Collagen-induced arthritis (CIA) and pristane-induced (PIA) rodents are commonly used experimental models reproduce many of pathogenic mechanisms human rheumatoid arthritis, i.e. increased cellular infiltration, synovial hyperplasia, pannus formation,...