A5101725657- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Genetic Mapping and Diversity in Plants and Animals
- Photosynthetic Processes and Mechanisms
- Berry genetics and cultivation research
- Plant Genetic and Mutation Studies
- Fluid Dynamics and Heat Transfer
- Horticultural and Viticultural Research
- Genetic diversity and population structure
- Agriculture, Soil, Plant Science
- Plant Gene Expression Analysis
- Bioenergy crop production and management
- Plant Surface Properties and Treatments
- Insect-Plant Interactions and Control
- Heavy Metals in Plants
- Aerosol Filtration and Electrostatic Precipitation
- Genetic and phenotypic traits in livestock
- CRISPR and Genetic Engineering
Beijing Forestry University
2022-2024
Ministry of Education of the People's Republic of China
2022
Research Institute of Forestry
2022
Abstract Background Autopolyploids, especially artificial lines, provide model systems for understanding the mechanisms of gene dosage effects on trait variation owing to their relatively uniform genetic background. Here, a protocol in vitro octaploid induction Populus hopeiensis from leaf blades with colchicine treatment was established through investigation different pre-culture durations, concentrations, and exposure times. Results We found that duration, concentration, time had...
The formation of diploid gametes through chromosome doubling is a major mechanism polyploidization, diversification, and speciation in plants. Unfavorable climate conditions can induce or stimulate the production during meiosis. Here, we demonstrated that heat shock stress (38°C for 3 6 h) induced 2n pollen formation, generated 42 triploids derived from shock-induced Populus canescens. Meiotic analysis treated mother cells revealed originated complete loss meiosis II (MII). Among triploids,...
Whole-genome duplication often results in a reduction the lignin content autopolyploid plants compared with their diploid counterparts. However, regulatory mechanism underlying variation remains unclear. Here, we characterize molecular after doubling of homologous chromosomes Populus hopeiensis. The showed that autotetraploid stems was significantly lower than its isogenic progenitor throughout development. Thirty-six differentially expressed genes involved biosynthesis were identified and...
The plant hormone gibberellin (GA) regulates many physiological processes, such as cell differentiation, elongation, seed germination, and the response to abiotic stress. Here, we found that injecting male flower buds with exogenous gibberellic acid (GA3) caused defects in meiotic cytokinesis by interfering radial microtubule array formation resulting restitution 2n pollen production Populus. A protocol for inducing Populus GA3 was established investigating effects of dominant stage,...
Genetic diversity analysis provides the scientific basis for preservation, evaluation, and utilization of germplasm resources tree species. We explored genetic structure Populus tomentosa elite trees in North China using 13 nuclear microsatellite markers. compared nine groups accessions including 20 originating from Beijing (BJ), 122 Hebei (HB), Shandong (SD), 113 Henan (HN), 270 Shanxi (SX), 54 Shaanxi (SAX), 8 Gansu (GS), 10 Anhui (AH), 6 Jiangsu (JS). All studied primer pairs were...
Primary trisomy is a powerful genetic tool in plants. However, has not been detected Populus as model system for tree and woody perennial plant biology.In the present study, backcross between alba × glandulosa 'YXY 7#' (2n = 2x 38) triploid hybrid 'Beilinxiongzhu 1#' 3x 57) based on observation of microsporogenesis an evaluation variations pollen was conducted to create primary trisomy. Many abnormalities, such premature migration chromosomes, lagging chromosome bridges, asymmetric...
Abstract Background Artificial induction of polyploidy is the most common and effective way to improve biological properties Populus develop new varieties this tree . In study, in order confirm expand earlier findings, we established a protocol using colchicine based on an efficient shoot regeneration system leaf blades induce tetraploidy vitro three genotypes from diploid hopeiensis The stomatal characteristics, blade size, growth were evaluated for diploids tetraploids genotypes. Results...
Aneuploidy in plants commonly presents as large variations morphology, physiology, biochemistry, and genetic effects owing to its karyotypic imbalance.
Abstract Background Artificial induction of polyploidy is the most common and effective way to improve biological properties Populus develop new varieties this tree. Here, a protocol using colchicine based on an efficient shoot regeneration system leaf blades was established for inducing tetraploidy in vitro from diploid hopeiensis . The traits that were evaluated each ploidy level stomatal characteristics, blade size, growth. Results We found genotype, preculture duration, concentration,...
High temperature can induce the production of 2n gametes and aborted pollen during microsporogenesis in Populus canescens. However, mechanism by which high induces abortion remains unknown. Here, was induced exposing male flower buds P. canescens to 38 41 °C; morphology, meiotic abnormalities, defects microtubular cytoskeleton, tapetum development were characterized. We found that dominant stage, temperature, duration treatment significantly affected percentage temperature-induced pollen....
A simulation study of droplet impingement on a moving film at different incidence angles in spray cooling application was conducted, and the following results were found: variation angle leads to alternating changes neck pressure difference flow field, which directly causes transition coronal morphology from asymmetric symmetric finally back an shape, with corresponding order appearance left right jets secondary droplets. After focusing temperature distribution heat flux variation, is found...