A5010982287- Ion channel regulation and function
- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Pancreatic function and diabetes
- Protein Kinase Regulation and GTPase Signaling
- Erythrocyte Function and Pathophysiology
- Receptor Mechanisms and Signaling
- Endoplasmic Reticulum Stress and Disease
- Nicotinic Acetylcholine Receptors Study
- Calcium signaling and nucleotide metabolism
- Extracellular vesicles in disease
- Venomous Animal Envenomation and Studies
- Pancreatitis Pathology and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA Interference and Gene Delivery
- Ion Channels and Receptors
- Growth Hormone and Insulin-like Growth Factors
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Mitochondrial Function and Pathology
- Nitric Oxide and Endothelin Effects
- Signaling Pathways in Disease
- MicroRNA in disease regulation
- RNA Research and Splicing
Vita-Salute San Raffaele University
2015-2024
University of Milano-Bicocca
1994-2024
San Raffaele University of Rome
2008-2021
Istituti di Ricovero e Cura a Carattere Scientifico
2019
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2019
IRCCS Ospedale San Raffaele
1995-2012
Italian Institute of Technology
2009-2011
Neuroscience Institute
2008-2010
Stem Cell Institute
2006
University of Lausanne
2005
Calsequestrin (CS) is the protein responsible for high-capacity, moderate affinity binding of Ca2+ within terminal cisternae sarcoplasmic reticulum, believed up to now be specific striated muscle. The cells two nonmuscle lines (HL-60 and PC12) rat tissues (liver pancreas) are shown here express a that resembles CS in many respects (apparent mass pH-dependent migration NaDodSO4/PAGE; blue staining with StainsAll dye; ability) specifically recognized by affinity-purified antibodies against...
The fluorescent indicator fura-2 has been applied to a variety of cell types in order set up appropriate conditions for measurements the cytosolic concentration free ionized Ca2+ [( Ca2+]i) both suspensions and single cells analyzed conventional fluorimeter or fluorescence microscope equipped quantitative analyses (with without computerized image analyses), respectively. When usual procedure dye loading (i.e., incubation at 37 degrees C with acetoxy-methyl ester) was used, often exhibited...
The subcellular components involved in the synthesis, transport, and discharge of secretory proteins guinea pig pancreatic exocrine cell have been isolated from gland homogenates by differential gradient centrifugation. They include rough smooth microsomes derived respectively endoplasmic reticulum Golgi periphery, a zymogen granule fraction consisting mainly mature granules smaller population condensing vacuoles, plasmalemmal fraction. Membrane subfractions were obtained particulate...
Autism spectrum disorder (ASD), affecting over 2% of the pre-school children population, includes an important fraction conditions accounting for heterogeneity autism. The disease was discovered 75 years ago, and present review, based on critical evaluations recognized ASD studies from beginning 1990, has been further developed by comparative analyses research clinical reports, which have grown progressively in recent up to late 2023. tools necessary identification its related pathologies...
The Ca2+ mobilization effect of inositol 1,4,5-trisphosphate, the second messenger generated via receptor-stimulated hydrolysis phosphatidylinositol 4,5-bisphosphate, is mediated by binding to intracellular receptors, which are expressed in high concentration cerebellar Purkinje cells. Partially conflicting previous reports localized receptor various subcellular structures: elements ER, both rough and smooth-surfaced, nuclear envelope, even plasma membrane. We have now reinvestigated problem...
Sympathetic neurons dissociated from the superior cervical ganglion of 2-day-old rats were studied by whole-cell patch clamp and fura-2 measurements cytosolic free Ca2+ concentration, [Ca2+]i. Step depolarizations in presence tetrodotoxin hexamethonium triggered two currents that differed voltage dependence activation kinetics inactivation. These resemble L N previously described chicken sensory [Nowycky, M. C., Fox, A. P. & Tsien, R. W. (1985) Nature (London) 316, 440-442]. Treatment...
The activity of BiP, the major chaperone endoplasmic reticulum (ER) lumen, is known to be Ca2+-regulated; however, participation this protein in ER storage cation has not yet been investigated. Here such a role demonstrated human epithelial (HeLa) cells transiently transfected with hamster BiP cDNA and incubated Ca2+-free medium, as revealed by two different techniques. In first, co-transfected aequorin was employed probe for assaying either cytosolic mitochondrial free Ca2+ concentration....
Rats infused with a supramaximally stimulating dose of the cholecystokinin-pancreozymin analogue caerulein develop acute interstitial pancreatitis (M. Lampel and H.F. Kern. Virchows Arch. A 373: 97-117, 1977). We have studied early (30-180 min) morphological changes in pancreatic acinar cells induced by infusing (2.5 micrograms X kg-1 h-1). The techniques thin-section electron microscopy, freeze fracture, enzyme immunocytochemistry were employed. Shortly (30 after onset infusion, large...
Macropinocytosis, a form of bulk uptake fluid and solid cargo into cytoplasmic vacuoles, called macropinosomes, has been studied mostly in relation to antigen presentation. Early membrane traffic events occurring this process are, however, largely unknown. Using human dendritic cells we show that marked increase the rate macropinocytosis occurs few minutes after application two markers (small latex beads or dextran), depends on slow intracellular Ca2+ concentration ([Ca2+](i)) rise precedes...
Dopaminergic D2 receptors are widely regarded as typical inhibitory receptors, they both inhibit adenylyl cyclase and decrease the cytosolic free Ca2+ concentration ([Ca2+]i) by activating K+ channels. A receptor has recently been cloned (Bunzow, J. R., Van Tol, H. M., Grandy, D. K., Albert, P., Salon, J., Christie, M. D., Machida, C. A., Neve, K. Civelli, O. (1988) Nature 336, 783-787) expressed in two different cell lines, pituitary GH4C1 cells Ltk- fibroblasts, where it shown to induce...
Single cell [Ca2+], studies were performed in chicken and rat osteoclasts loaded with fura-2 exposed to a variety of treatments. Under resting conditions, basal [Ca2+]i, was 79.2 +/- 47.3 84.3 65.7 nM (averages S.D.; n = 141 126) the two species, respectively. Basal [Ca2+]i stable all approximately 80% osteoclasts. In remaining 20%, spontaneous, irregular fluctuations observed (amplitude range: 50-200 nm over values). Increase [Ca2+]o concentration Krebs-Ringer incubation medium (2 mM)...
Incubation of guinea pig pancreatic lobules in Ca++-free Krebs-Ringer bicarbonate solution (KRB) containing 0.5 mM ethylene glycol-bis(beta-aminoethyl ether)N,N,N',N'-tetraacetate (EGTA) results the progressive fragmentation occluding zonulae (ZO) with formation multiple discrete junctions (fasciae occludentes) localized lateral and lumenal plasmalemma. After 1--2 h such incubation, most ZO appear completely disassembled. This disappearance heterogeneity density intramembrane particles on...