A5064896125- Environmental DNA in Biodiversity Studies
- Marine Ecology and Invasive Species
- Microbial Community Ecology and Physiology
- Identification and Quantification in Food
- Marine Biology and Ecology Research
- Aquatic Invertebrate Ecology and Behavior
- Marine and fisheries research
- Marine Bivalve and Aquaculture Studies
- Protist diversity and phylogeny
- Isotope Analysis in Ecology
- Coral and Marine Ecosystems Studies
- Microplastics and Plastic Pollution
- Marine Biology and Environmental Chemistry
- Coastal and Marine Management
- Marine and coastal ecosystems
- Parasite Biology and Host Interactions
- Fish Ecology and Management Studies
- Marine and coastal plant biology
- Recycling and Waste Management Techniques
- Soil and Water Nutrient Dynamics
- Marine and environmental studies
- Water-Energy-Food Nexus Studies
- Molecular Biology Techniques and Applications
- Aquatic Ecosystems and Phytoplankton Dynamics
- Pharmaceutical and Antibiotic Environmental Impacts
Cawthron Institute
2016-2025
University of Auckland
2017-2024
Klaipėda University
2013-2023
Norwegian Institute of Marine Research
2021
Scion
2021
Moss Landing Marine Laboratories
2021
The brackish Baltic Sea hosts species of various origins and environmental tolerances. These immigrated to the sea 10,000 15,000 years ago or have been introduced area over relatively recent history system. has only one known endemic species. While information on some abiotic parameters extends back as long five centuries first quantitative snapshot data biota (on exploited fish populations) originate generally from same time, international coordination research began in early twentieth...
Targeted species-specific and community-wide molecular diagnostics tools are being used with increasing frequency to detect invasive or rare species. Few studies have compared the sensitivity specificity of these approaches. In present study environmental DNA from 90 filtered seawater 120 biofouling samples was analyzed quantitative PCR (qPCR), droplet digital (ddPCR) metabarcoding targeting cytochrome c oxidase I (COI) 18S rRNA genes for Mediterranean fanworm Sabella spallanzanii. The qPCR...
High-throughput sequencing metabarcoding studies in marine biosecurity have largely focused on targeting environmental DNA (eDNA). can persist extracellularly the environment, making discrimination of living organisms difficult. In this study, bilge water samples (i.e., accumulating on-board a vessel during transit) were collected from 15 small recreational and commercial vessels. eDNA eRNA molecules co-extracted V4 region 18S ribosomal RNA gene targeted for metabarcoding. total, 62.7%...
The human-mediated introduction of marine non-indigenous species is a centuries- if not millennia-old phenomenon, but was only recently acknowledged as potent driver change in the sea. We provide synopsis key historical milestones for bioinvasions, including timelines (a) discovery and understanding invasion process, focusing on transfer mechanisms outcomes, (b) methodologies used detection monitoring, (c) approaches to ecological impacts research, (d) management policy responses. Early...
To enable successful management of marine bioinvasions, timely and robust scientific advice is required. This knowledge should inform managers stakeholders on the magnitude a pressure (rate human-mediated introductions), environmental state an ecosystem (impacts non-indigenous species), success response (prevention, eradication, mitigation). often relies baseline biodiversity information in form measureable parameters (metrics). can be derived from conventional approaches such as visual...
In the marine sciences an increasing number of studies on environmental changes, their causes, and assessments emerged in recent years. Often authors use non-uniform inconsistent definitions key terms like driver, threats, pressures etc. Although all these clearly define causal dependencies between interacting socio-economic systems understandable way, still overall imprecise wording could induce misunderstanding at higher policy levels when it comes to integrated ecosystems assessments....
The field of eDNA is growing exponentially in response to the need for detecting rare and invasive species management conservation decisions. Developing technologies standard protocols within biosecurity sector must address myriad challenges associated with marine environments, including salinity, temperature, advective deposition processes, hydrochemistry pH, contaminating agents. These approaches also provide a robust framework that meets decisions regarding threats human health,...
Abstract Environmental DNA (eDNA)‐based methods are increasingly used by government agencies to detect pests and threatened species, for broader biodiversity monitoring. Given rapid technological advances a growing number of commercial service providers, there is need standardize quality assurance maintain confidence in eDNA‐based results. Here, we introduce two documents provide best‐practice guidelines Australian New Zealand eDNA researchers end‐users (available from...
In recent decades, the melting of glaciers has led to a consistent increase in number periglacial coastal lagoons that form place receding Svalbard, European Arctic. There is limited data on geomorphology and hydrology these novel formations, primarily because conducting research remote polar regions logistically challenging expensive. We present hydrological bathymetric collected 2022-2024 newly formed lagoon located western part Spitsbergen (Svalbard), between Eidembreen glacier Eidembukta...
Abstract Epigenetics, as a DNA signature that affects gene expression and enables rapid reaction of an organism to environmental changes, is likely involved in the process biological invasions. methylation epigenetic mechanism common plants animals for regulating expression. In this study we show, first time any marine species, significant reduction global levels during expansive phase pygmy mussel ( Xenostrobus securis ) recent invasion Europe (two-year old), while older introductions such...
A Catalogue of Marine Biodiversity Indicators was developed with the aim providing basis for assessing environmental status marine ecosystems. Useful implementation Strategy Framework Directive (MSFD), this catalogue allows navigation a database indicators mostly related to biological diversity, non-indigenous species, food webs, and seafloor integrity. Over 600 were compiled, which used in framework different initiatives (e.g. EU policies, research projects) national international contexts...
Environmental DNA is increasingly being used in marine invasive species surveillance despite the inability to discriminate between contemporary intracellular (i.e. living) and extracellularly persistent legacy) fragments. RNA emerging as a powerful alternative when distinguishing living portion of community essential. A positive relationship signals may justify use only for more rapid cost-effective detections. In this study environmental were co-extracted from settlement plates water...
Wide-ranging, indicator-based assessments of large, complex ecosystems are playing an increasing role in guiding environmental policy and management. An example is the EU's Marine Strategy Framework Directive, which requires Member States to take measures reach "good status" (GES) European marine waters. However, formulation indicator targets consistent with Directive's high-level goal sustainable use has proven challenging. We develop a specific, quantitative interpretation concepts GES...
In this experimental study the patterns in early marine biofouling communities and possible implications for surveillance environmental management were explored using metabarcoding, viz. 18S ribosomal RNA gene barcoding combination with high-throughput sequencing. The community structure of eukaryotic assemblages initial succession assessed from settlement plates deployed a busy port one, five 15 days. metabarcoding results verified traditional morphological identification taxa selected...
Abstract Marine infrastructure can favor the spread of non-indigenous marine biofouling species by providing a suitable habitat for them to proliferate. Cryptic organisms or those in early life stages be difficult distinguish conventional morphological taxonomy. Molecular tools, such as metabarcoding, may improve their detection. In this study, ability morpho-taxonomy and metabarcoding (18S rRNA COI) using three reference databases (PR2, BOLD NCBI) characterize biodiversity detect (NIS) was...
Abstract Incidental detection of species concern (e.g., invasive species, pathogens, threatened and endangered species) during biodiversity assessments based on high‐throughput DNA sequencing holds significant risks in the absence rigorous, fit‐for‐purpose data quality reporting standards. Molecular are predominantly collected for ecological studies thus generated to common assurance However, certain these would likely elicit interest from end users working biosecurity or other surveillance...
Advances in high-throughput sequencing (HTS) are revolutionizing monitoring marine environments by enabling rapid, accurate and holistic detection of species within complex biological samples. Research institutions worldwide increasingly employ HTS methods for biodiversity assessments. However, variance laboratory procedures, analytical workflows bioinformatic pipelines impede the transferability comparability results across research groups. An international experiment was conducted to...
Abstract Growing interest and affordability of environmental DNA RNA (eDNA eRNA) approaches for biodiversity assessments monitoring complex ecosystems have led to the emergence manifold protocols nucleic acids (NAs) isolation processing. Although there is no consensus on a standardized workflow, common practice water samples concentrate NAs via filtration using varying pore size membranes. Using smallest assumed be most efficient capture from wide range material (including sub‐cellular...