A5023377696- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Animal Behavior and Reproduction
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Analytical Chemistry and Sensors
- bioluminescence and chemiluminescence research
- Olfactory and Sensory Function Studies
- Electrochemical Analysis and Applications
- Gas Sensing Nanomaterials and Sensors
- Circadian rhythm and melatonin
- Genetics, Aging, and Longevity in Model Organisms
- Silicon Nanostructures and Photoluminescence
- Advanced Photocatalysis Techniques
- Invertebrate Immune Response Mechanisms
- Cellular transport and secretion
- Insect Utilization and Effects
- Physiological and biochemical adaptations
- RNA Interference and Gene Delivery
- Semiconductor materials and devices
- Nanowire Synthesis and Applications
- TiO2 Photocatalysis and Solar Cells
- Transition Metal Oxide Nanomaterials
Institut des Neurosciences Paris-Saclay
2016-2024
CEA Paris-Saclay
2020-2024
Université Paris-Saclay
2020-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2020-2024
Centre National de la Recherche Scientifique
2010-2024
CEA Paris-Saclay - Etablissement de Saclay
2024
Université Paris-Sud
2001-2018
Laboratoire de Neurobiologie Cellulaire et Moléculaire
2006-2012
École Centrale de Lyon
1994-2007
Institut de Microélectronique, Electromagnétisme et Photonique
2006
Homo-oligomer DNA strands were immobilized onto silicon/silicon dioxide electrodes using 3-aminopropyltriethoxysilane. These modified substrates used as working in a three-electrode electrochemical cell. In-phase and out-of-phase impedances measured the range −1 to +1 V with respect an Ag/AgCl reference electrode, superimposed 10 mV ac signal at frequencies of 20 100 kHz. Ex situ hybridization complementary oligomer strands, performed surface electrodes, is clearly reflected by negative...
Locomotor activity of single, freely walking flies in small tubes is analyzed the time domain several hours. To assess influence mushroom bodies on activity, three independent noninvasive methods interfering with body function are applied: chemical ablation precursor cells; a mutant affecting Kenyon cell differentiation (mushroom miniature); and targeted expression catalytic subunit tetanus toxin subsets cells. All groups defects show an elevated level total activity. This increase...
Adipokinetic hormone (AKH) is a metabolic neuropeptide principally known for its mobilization of energy substrates, notably lipid and trehalose during energy-requiring activities, such as flight locomotion. Drosophila melanogaster AKH cell localization in corpora cardiaca, other insect species, was confirmed by immunoreactivity genetic approach using the UAS/GAL4 system. To assess general physiological rules, we ablated endocrine cells specifically driving expression apoptosis transgenes...
The hormonal regulation of feeding behaviour is well known in vertebrates, whereas it remains poorly understood insects. Here, we report that the takeout gene an essential component nutritional homeostasis Drosophila. encodes a putative juvenile hormone (JH) binding protein and has been described as link between circadian rhythm behaviour. However, physiological role its to JH remain unknown. In this study, show (to(1)) flies failed adapt their food intake according availability most defects...
SUMMARY The central complex is one of the most prominent neuropils in insect brain. It has been implicated control locomotor activity and considered as a pre-motor center. Several neuropeptides are expressed circuits complex, thus may be modulators behavior. Here we have investigated roles two different neuropeptides, Drosophila tachykinin (DTK) short neuropeptide F (sNPF), aspects In brain, DTK sNPF interneurons innervating complex. We directed RNA interference (RNAi) towards specifically...
Vertebrate synapsins are abundant synaptic vesicle phosphoproteins that have been proposed to fine-regulate neurotransmitter release by phosphorylation-dependent control of motility. However, the consequences a total lack all synapsin isoforms due knock-out three mouse genes not yet investigated. In Drosophila single gene encodes several and is expressed in most terminals. Thus targeted deletion eliminates possibility functional complementation other isoforms. Unexpectedly, null mutant flies...
The insulin signaling pathway has been implicated in several physiological and developmental processes. In mammals, it controls expression of 3-Hydroxy-3-Methylglutaryl CoA Reductase (HMGCR), a key enzyme cholesterol biosynthesis. insects, which can not synthesize de novo, the HMGCR is biosynthesis juvenile hormone (JH). However, link between JH established. Drosophila, mutations receptor (InR) decrease rate synthesis. It also known that both play role control sexual dimorphism locomotor...
Insulin signaling pathways are implicated in several physiological processes invertebrates, including the control of growth and life span; latter these has also been correlated with juvenile hormone (JH) deficiency. In turn, JH levels have sex-specific differences locomotor activity. Here, involvement insulin pathway activity was investigated Drosophila. Ablation insulin-producing neurons adult pars-intercerebralis found to increase trehalosemia abolish sexual dimorphism relevant locomotion....
Many different cells' signalling pathways are universally regulated by Ca2+ concentration [Ca2+] rises that have highly variable amplitudes and kinetic properties. Optical imaging can provide the means to characterise both temporal spatial aspects of signals involved in neurophysiological functions. New methods for vivo brain Drosophila required probing dynamic this system. In studies here, whole was performed on transgenic flies with targeted expression bioluminescent reporter GFP-aequorin...
In Drosophila , locomotor activity is sexually dimorphic and the brain area controlling this dimorphism has been mapped. The neurons of pars intercerebralis (PI) have suggested to participate in such differences between males females. However, precise physical nature dimorphism, identity PI involved, neuronal signal coding remain unknown. study, we used a video-tracking paradigm characterize further pattern . We show that number activity/inactivity periods (start/stop bouts) also dimorphic,...
Abstract The Drosophila larva is widely used for studies of neuronal development and function, yet little known about the basis locomotion in this model organism. larvae crawl over a plain substrate by performing repetitive waves forward peristalsis alternated brief episodes head swinging turning. To identify sets central peripheral neurons required spatial or temporal pattern larval locomotion, we blocked neurotransmitter release from defined populations targeted expression tetanus toxin...
The temporal properties of a variety behavioral traits obey power law distributions, property often referred to as fractal. We recently showed that the pattern locomotor activity fruitfly Drosophila melanogaster follows this distribution. Although an increasing number such fractal patterns are being discovered, brain areas and neuronal networks responsible remain unknown. In study, we show specifically blocking synapses established by neurons ellipsoid-body, substructure central complex in...