Abstract Extracellular vesicles (EVs) derived from human adipose-derived stem cells (hADSCs) possess the proangiogenic potential for ischaemic diseases. Thus, our study aimed to evaluate therapeutic effects of hADSC-EVs on fat grafting and explore mechanism promoting angiogenesis. The EVs released by hADSCs incubated under normal or hypoxic conditions were employed supplement in a nude mouse model. proliferation, migration, tube formation vascular endothelial growth factor (VEGF) secretion...

Atopic dermatitis is defined as an intensely systemic inflammation among skin diseases. Exosomes derived from adipose-derived stem cells may be a novel cell-free therapeutic strategy for atopic treatment. This study aims to elucidate the possible underlying mechanism of cells-exosomes harboring microRNA-147a in pathogenesis. BALB/c mice treated with Dermatophagoides farinae extract/2,4-dinitrochlorobenzene were mouse model dermatitis, either inflamed HaCaT and HUVECs exposed TNF-α/IFN-γ...

Background Preventing scar formation during wound healing has important clinical implications. Numerous studies have indicated that adipose-derived stem cell culture mediums, which are rich in cytokines and extracellular vesicles (EVs), regulate matrix remodeling prevent after healing. Therefore, using a rabbit model, we tried to demonstrate factor mediums plays major role preventing (EVs or cytokines), as well revealing the underlying mechanism. Methods Human cells (hASCs) were isolated...

BACKGROUND Diabetic wound injury is a significant and common complication in individuals with diabetes. N6-methyladenosine (m6A)-related epigenetic regulation widely involved the pathogenesis of diabetes complications. However, function m6A methyltransferase Wilms tumor 1-associated protein (WTAP) diabetic healing remains elusive. AIM To investigate potential regulatory mechanism WTAP during healing. METHODS Human umbilical vein endothelial cells (HUVECs) were induced high glucose (HG) to...

Background: Growing evidence has demonstrated that adipose-derived stem cell-derived extracellular vesicles enhance the survival of fat grafts and browning white adipose tissue. We evaluated whether supplementation with promotes grafts. Methods: Extracellular derived from cells were injected into C57BL/6 mice once per week until postgraft 12. The collected weighed after weeks 2, 4, histological morphology, neovascularization, proportion M2 macrophages evaluated. ability to promote macrophage...

To investigate the effects of adipose-derived stem cell released exosomes (ADSC-Exos) on wound healing in diabetic mice.The ADSCs were isolated from adipose tissue donated by patients and cultured enzymatic digestion. The supernatant 3rd generation was used to extract Exos (ADSC-Exos). morphology ADSC-Exos observed transmission electron microscopy. membrane-labeled proteins (Alix CD63) detected Western blot, particle size distribution nanoparticle tracking analyzer. fibroblasts skin 5th with...

Sir: We thank Drs. Zhao and Sun for their insightful comments on our article entitled “Supplementation with Extracellular Vesicles Derived from Adipose-Derived Stem Cells Increases Fat Graft Survival Browning in Mice: A Cell-Free Approach to Construct Beige White Grafting.”1 ask detailed information about this study as follows: (1) How are the dose frequency of extracellular vesicle injection determined? (2) Why use vesicle–derived human lipoaspirates rather than adipose tissue mice? As...

Background: Multiple studies have demonstrated that mechanical forces promote the growth of adipose tissue. However, mechanism tissue regeneration induced by remains unclear. Methods: In an experiment using rats, prefabricated with a vessel pedicle was expanded external volume expansion device. The fat flaps tested at different time points. Cell proliferation and angiogenesis were analyzed immunofluorescence. expression adipogenic genes inflammatory cytokines evaluated real-time polymerase...

Objective To explore the effects of adipose-derived stem cell released exosomes (ADSC-Exos) on proliferation, migration, and tube-like differentiation human umbilical vein endothelial cells (HUVECs). Methods Adipose tissue voluntarily donated by liposuction patients was obtained. The ADSCs were harvested enzyme digestion identified flow cytometry adipogenic induction. ADSC-Exos extracted from supernatant 3rd generation morphology observed transmission electron microscopy. surface proteins...

Currently, there is a lack in therapy that promotes the reepithelialization of diabetic wounds as an alternative to skin grafting. Here, authors hypothesized extracellular vesicles from adipose-derived stem cells (ADSC-EVs) could accelerate wound closure through rescuing function keratinocytes mice.The effect ADSC-EVs on biological human keratinocyte was assayed vitro. In vivo, 81 male severe combined immune deficiency mice aged 8 weeks were divided randomly into vesicle-treated diabetes...

Subcutaneous transplantation of decellularized adipose tissue was capable recellularization during soft repair. However, further improvements are required to promote angiogenesis and adipogenesis. Here, the authors proposed a neo-mechanical protocol isolate tissue-derived extracellular vesicles (ATEVs) through lipoaspirate as mediator for both adipogenesis, prepared ATEV-rich hydrogel engineering.Adipose liquid extract lipid-devoid were extracted homogenization repeated freeze thaw cycles....

Objective Diabetic ulcers are a prevalent complication of diabetes mellitus and represent one the most complex severe complications that can occur in diabetic patients. Most existing studies have separately examined neutrophil-to-lymphocyte ratio (NLR) prognostic value or platelet-to-lymphocyte (PLR). However, to our knowledge, no study has evaluated relationship between monocyte-to-lymphocyte (MLR) non-healing lower extremity (NHLU) This explored association 3 hematological parameters (MLR,...

Abstract Osteosarcoma (OS) is a highly malignant tumor, and its dysregulated lipid metabolism associated with tumorigenesis unfavorable prognosis. Interestingly, long noncoding RNAs (lncRNAs) have emerged as pivotal regulators of metabolism, exerting notable impacts on tumor proliferation. Nevertheless, the involvement RPARP-AS1, novel metabolism-associated lncRNA, remains unexplored in context OS. This study aims to identify functionally relevant lncRNAs impacting OS proliferation seeks...

To investigate the effect of adipose-derived stem cell derived exosomes (ADSC-Exos) on angiogenesis after skin flap transplantation in rats.ADSCs were isolated and cultured by enzymatic digestion from voluntary donated adipose tissue patients undergoing liposuction. The 3rd generation cells observed under microscopy identified flow cytometry oil red O staining at 14 days induction adipogenesis. After as ADSCs, ADSC-Exos was extracted density gradient centrifugation. And morphology...

Background Random flaps are widely used for wound repair. However, flap necrosis is a serious complication leading to the failure of operation. Our previous study demonstrated great proangiogenic potential hypoxia-treated adipose-derived stem cells–extracellular vesicles (HT-ASC-EVs). Thus, we aim evaluate effect HT-ASC-EVs in survival and angiogenesis random skin rats. Methods Adipose-derived were respectively isolated from cell culture medium 3 donors via ultracentrifugation. The...

Background: The preoperative design for the amount of skin excision upper eyelid is a common procedure in Asian blepharoplasty, but there lack an effective method addressing dermatochalasis to acquire esthetically pleasing results. Our aim was propose alternative technique determine combined with esthetics eyes correcting laxity and therefore create attractive double eyelids. Materials Methods: invaginating-simulating esthetic criteria determining excised were performed during...

Clinically unpredictable retention following fat grafting remains outstanding problems because of the unrevealed mechanism grafted survival. The role autophagy, a process to maintain cellular homeostasis through recycling debris, has yet been be reported in grafting. This study aims improve survival autophagy. First, relationship between cell death and autophagy early stage was evaluated immunostaining, RNA sequencing, western blot. Next, rapamycin, an autophagic agonist, used for culturing...

The healing of diabetic wounds is poor due to a collagen deposition disorder. Matrix metalloproteinase-9 (MMP-9) closely related in the process tissue repair. Many studies have demonstrated that extracellular vesicles derived from adipose-derived stem cells (ADSC-EVs) promote wound by enhancing deposition.In this study, we explored whether ADSC-EVs could downregulate expression MMP-9 and improving deposition. potential effects on were tested both vitro vivo.We first evaluated effect...

To investigate the clinical effect of double insurance method flexible suspension and semiflexible in bionic blepharoplasty.

Objective: To assess the clinical efficacy of combining autologous fat grafting with hair follicle unit transplantation. Methods: The authors conducted a retrospective analysis involving 30 patients at Department Plastic Surgery, Second Affiliated Hospital Nanchang University, between January 2021 and 2023. Granular was harvested from thigh’s posterior aspect using liposuction. then filtered through cotton pads, fascia excised, implanted into scar tissue. Postoperatively, beyond 3-month...

To explore the possibility of constructing tissue engineered adipose by derived extracellular vesicles (hAT-EV) combined with decellularized (DAT) scaffolds, and to provide a new therapy for soft defects.The voluntarily donated liposuction patient was divided into two parts, one them observed HE Masson staining scanning electron microscope (SEM). Immunohistochemical Western blot detection collagen type Ⅰ Ⅳ laminin were also employed. Another incubated exosome-removed complete medium 48...