Epidermal growth factor receptor (EGFR)-targeted drugs (erlotinib, etc.) are used to treat multiple types of tumours. EGFR is highly expressed in most triple-negative breast cancer (TNBC) patients. However, only a small proportion TNBC patients benefit from EGFR-targeted clinical trials, and the resistance mechanism unclear. Here, we found that PDZ domain containing 1 (PDZK1) downregulated erlotinib-resistant cells, suggesting PDZK1 downregulation related erlotinib TNBC. binds EGFR. Through...

Metabolic reprogramming is the prominent feature of clear cell renal carcinoma (ccRCC). Succinate dehydrogenase subunit B (SDHB) one subunits mitochondrial respiratory chain complex II. The loss SDHB function closely related with metabolic changes in kidney cancer cells. However, role and molecular mechanism ccRCC occurrence progression are still unclear. In this study, results bioinformatics analyses on GEO, TCGA oncomine databases immunohistochemistry showed that expression level was...

MicroRNAs (miRNAs) were reported to be involved in the development of clear cell renal carcinoma (ccRCC). However, study on miRNAs ccRCC is far from complete. The present identified which could act as potential novel prognostic markers for ccRCC, and analyzed its possible mechanism. We found that miR-19a correlated with poor prognosis patients via promoting proliferation suppressing PTEN/SMAD4 expression. Both microarray screening result TCGA KIRC dataset analysis showed was significantly...

Abstract Precision therapy for clear cell renal carcinoma (cc RCC ) requires molecular biomarkers ascertaining disease prognosis. In this study, we performed integrated proteomic and transcriptomic screening in all four tumour‐node‐metastasis stages of cc adjacent normal tissues ( n = 18) to investigate differentially expressed genes. Most identified genes revealed a strong association with transforming growth factor‐β level the epithelial‐to‐mesenchymal transition process. Of them, Serpin...

Clear cell renal carcinoma (ccRCC) is the most lethal neoplasm of urologic system. Clinical therapeutic effect varies greatly between individual ccRCC patients, so there an urgent need to develop prognostic molecular biomarkers help clinicians identify patients in early aggressive management. In this study, samples from primary tumor and their corresponding nontumor adjacent tissues (n=18) were analyzed by quantitative proteomic assay. Proteins downregulated tumors studied GO KEGG pathways...

Abstract Insufficient understanding about the immune evasion mechanism leads to inability in predicting current immunotherapy effects clear cell renal carcinoma (ccRCC) and sensitizing ccRCC immunotherapy. RNA binding proteins (RBPs) can promote tumor progression evasion. However, research on RBPs, particularly m6A reader YTHDF3, development is limited. In this study, we found that YTHDF3 level was downregulated an independent prognostic biomarker for ccRCC. Decreased expression correlated...

The Multiple T-maze (MTM) and the Barnes maze (BM) are land mazes used for evaluation of spatial memory. observation that mice performing differently in individual made us test hypothesis differences cognitive performances two would be accompanied by hippocampal protein levels. C57BL/6J were tested BM MTM, hippocampi extirpated 6 h following probe trials each, proteins extracted gel-based proteomic analysis. Mice learned task both paradigms. Levels from several pathways including signaling,...

Ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50) suppresses breast cancer cell proliferation, potentially through its regulatory effect on epidermal growth factor receptor (EGFR) signaling, although the mechanism by which this occurs remains unknown. Thus in our studies, we aimed to determine of EBP50 expression EGF-induced proliferation and activation EGFR signaling lines, MDA-MB-231 MCF-7. In cells, express low levels EBP50, overexpression inhibited ERK1/2 AKT phosphorylation. MCF-7...

Background: MicroRNAs (miRNAs) have been linked to a number of cancer types including breast cancer. The rate brain metastases is 10-30% in patients with advanced which associated poor prognosis. potential application miRNAs the diagnostics and therapeutics metastasis an area intense interest. In initial effort systematically address differential expression mRNAs primary may provide clues for early detection metastasis, we analyzed consequent changes global patterns gene Gene Expression...

The actin cytoskeleton is composed of a highly dynamic network filamentous proteins, yet the molecular mechanism that regulates its organization and remodeling remains elusive. In this study, Na(+)/H(+) exchanger regulatory factor (NHERF)-1 loss-of-function gain-of-function experiments reveal polymerized (F-actin) in HeLa cells disorganized by NHERF1, whereas protein expression levels exhibit no detectable change. To elucidate underlying disorganization combined 2-dimensional...

This Phase II study was conducted to evaluate the activity and feasibility of a regimen nedaplatin 5-fluorouracil as induction chemotherapy, followed by intensity-modulated radiotherapy concurrent with chemotherapy in patients locoregionally advanced nasopharyngeal carcinoma.Patients received neoadjuvant comprised two cycles at 700 mg/m(2)/day administered on days 1-4 continuous intravenous infusion (100 mg/m(2) i.v. over 2 h) given after administration day 1, repeated every 3 weeks,...

Clear cell renal carcinoma (ccRCC) is the most common and lethal cancer of adult kidney. However, its pathogenesis has not been fully understood till now, which hinders therapeutic development ccRCC. NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4-like 2 (NDUFA4L2) was found to be upregulated play an important role in We aimed further investigate underlying mechanisms by NDUFA4L2 exerted function expression level upregulated.The Gene Expression Omnibus (GEO) The Cancer Genome Atlas...

Clear cell renal carcinoma (ccRCC) is a highly immunogenic tumor and immune dysfunction associated with ccRCC poor prognosis. The RhoGTPase-activating proteins (RhoGAPs) family was reported to affect development, but its role in immunity prognosis prediction for remain unknown. In the current study, we found ARHGAP11A only independent risk factor among 33 RhoGAPs (hazard ratio [HR] 1.949, 95% confidence interval [CI] 1.364-2.785). High level shorter overall survival (OS, HR 2.040, CI...

MINT‐7716320, MINT‐7716422, MINT‐7716502, MINT‐7716450, MINT‐7716470: beta2AR (uniprotkb:P07550) binds (MI:0407) to MAGI‐3 (uniprotkb:Q5TCQ9) by pull down (MI:0096)

The actin cytoskeleton plays an important role in cell shape determination, adhesion and cycle progression. Ezrinradixin-moesin (ERM)-binding phosphoprotein 50 (EBP50), also known as Na(+)-H(+) exchanger regulatory factor 1 (NHERF1), associates with is related to Its Ser279 Ser301 residues are phosphorylated by cyclin-dependent kinase 2 (cdc2)/cyclin B during the mitosis phase. However, biological significance of EBP50 phosphorylation mediated cdc2/cyclin not clear. In present study,...

// Ran Meng 1, * , Qiong Qin 2, Ying Xiong 2 Yan Wang 1 Junfang Zheng Yuan Zhao Tao Qiqi Hua Liu Songlin 3 Wen G. Jiang 4 Junqi He Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing, China Beijing Key Laboratory for Tumor Invasion Metastasis, International Cooperation Base Science Technology on China-UK Cancer Research, Gene Therapy Tooth Regeneration, University School Stomatology, Cardiff Research Collaborative, Medicine, Cardiff, United Kingdom These...

Although the management of patients with renal cell carcinoma (RCC) has changed drastically in recent years, it is still faced evolving challenge. Elucidation mechanisms underlying RCC will help development therapies, as well biomarkers for early diagnosis. In this study, ccRCC tissues from different stages were subject to iTRAQ-based proteomics analysis. 130 common differentially expressed proteins (DEPs) found and lipid metabolism pathway was obviously dysregulated all stages. These DEPs...